Cat. No.: HY-N1970

5,7-Dihydroxychromone

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

5,7-Dihydroxychromone is a flavonoid compound with antioxidant properties. 5,7-Dihydroxychromone induces Nrf2 nuclear translocation, increases Nrf2/ARE binding activity, and up-regulates Nrf2-dependent antioxidant genes HO-1, NQO1, GCLc. 5,7-Dihydroxychromone attenuates excessive ROS generation, inhibits activated caspase-3, caspase-9, cleaved PARP expression, and prevents neuronal apoptosis and cell death. 5,7-Dihydroxychromone increases LXRα and PPARγ mRNA expression, induces preadipocyte differentiation, and regulates blood glucose levels. 5,7-Dihydroxychromone inhibits radial growth of soil pathogenic fungi, radicle elongation of select seedlings, and transiently inhibits Bradyrhizobium sp. growth in high mannitol medium. 5,7-Dihydroxychromone can be used for the research of Parkinson’s disease, type 2 diabetes mellitus and pathogenic fungal infection.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 31721-94-5
Formula: C₉H₆O₄
Molecular Weight:178.14

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

5,7-Dihydroxychromone Chemical Structure

CAS No.: 31721-94-5

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Caspase-3

Caspase-9

PARP

All Caspase Isoforms :

All PARP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
SH-SY5Y	EC₅₀	1.9 μM Compound: 24	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 6-OHDA-induced cell death measured after 24 hrs by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 6-OHDA-induced cell death measured after 24 hrs by MTT assay	[PMID: 27420919]

In Vitro

5,7-Dihydroxychromone (DHC) (0.4-10 μM; 24 h pre-incubation) dose-dependently protects SH-SY5Y cells from 6-OHDA (HY-B1081)-induced cell death, and this effect is eliminated by Nrf2 siRNA transfection (48 h pre-transfection)^[1].
5,7-Dihydroxychromone (DHC) (0.4-10 μM; 24 h pre-incubation) dose-dependently inhibits 6-OHDA-induced intracellular ROS generation in SH-SY5Y cells, with 10 μM DHC reducing ROS levels to ~2-fold above the vehicle control, and this effect is eliminated by Nrf2 siRNA transfection (48 h pre-transfection)^[1].
5,7-Dihydroxychromone (DHC) (2 μM; 1-12 h) time- and (0.08-10 μM; 6 h) dose-dependently induces Nrf2 nuclear translocation in SH-SY5Y cells, with 2 μM DHC producing a peak effect at 6 h and 0.08 to 10 μM DHC increasing nuclear Nrf2 levels in a dose-dependent manner at 6 h^[1].
5,7-Dihydroxychromone (DHC) (2 μM; 1-12 h) increases Nrf2/ARE binding activity in SH-SY5Y cells, with 2 μM DHC producing maximum binding activity at 12 h^[1].
5,7-Dihydroxychromone (DHC) (2 μM; 1-24 h) time- and (0.08-10 μM; 24 h) dose-dependently increases protein expression of the Nrf2-dependent antioxidant enzymes HO-1, NQO1, and GCLc in SH-SY5Y cells, and this effect is eliminated by Nrf2 siRNA transfection (48 h pre-transfection)^[1].
5,7-Dihydroxychromone (DHC) (0.08-10 μM; 24 h pre-incubation) dose-dependently inhibits 6-OHDA-induced expression of cleaved caspase-3, cleaved caspase-9, and cleaved PARP in SH-SY5Y cells^[1].
5,7-Dihydroxychromone (1-10 μg/mL; 9 d) potently induces differentiation of mouse 3T3-L1 preadipocytes in a concentration-dependent manner, with 10 μg/mL driving differentiation to 185% of control^[2].
5,7-Dihydroxychromone (1-10 μg/mL) increases PPARγ and LXRα mRNA expression in mouse 3T3-L1 preadipocytes in vitro in a concentration-dependent manner, with 10 μg/mL driving 30-fold and 22-fold increases respectively^[2].
5,7-Dihydroxychromone (5-100 μM; until maximum radial growth reached) inhibits radial growth of Rhizoctonia solani (IC₅₀ = 18 μM) and Sclerotium rolfsii (IC₅₀ = 26 μM), with fungicidal activity at 100 μM^[3].
5,7-Dihydroxychromone (10^-7-10^-3 M; 48 hr) inhibits radicle elongation of velvetleaf (IC₅₀ = 30 μM), corn (cv. DeKalb IL 645-786; IC₅₀ = 50 μM), peanut (cv. Red Tennessee; IC₅₀ = 65 μM), and wheat (cv. Cardinal; IC₅₀ = 200 μM) after 48 hr of incubation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

human neuroblastoma SH-SY5Y cells
Concentration:

0.4 μM, 2 μM, 10 μM; 10 μM (with Nrf2 siRNA pre-transfection)
Incubation Time:

24 h (pre-incubation); 48 h (Nrf2 siRNA pre-transfection)
Result:

Reduced 6-OHDA-induced cell death in a dose-dependent manner.
Decreased the percentage of PI-positive dead cells from 44.5% (6-OHDA-only group) to 13.8% at 10 μM.
Abolished the protective effect when Nrf2 siRNA was transfected, increasing the percentage of PI-positive cells to 50.1% in the 10 μM DHC + 6-OHDA group.

Western Blot Analysis^[1]

Cell Line:

human neuroblastoma SH-SY5Y cells
Concentration:

2 μM (time-course); 0.08-10 μM (dose-response)
Incubation Time:

1-12 h (2 μM); 6 h (0.08-10 μM)
Result:

Increased nuclear Nrf2 levels in a time-dependent manner at 2 μM, with a peak effect at 6 h.
Increased nuclear Nrf2 levels in a dose-dependent manner after 6 h treatment with 0.08-10 μM.
Showed increased nuclear Nrf2 fluorescence via immunocytochemical staining.

Western Blot Analysis^[1]

Cell Line:

human neuroblastoma SH-SY5Y cells
Concentration:

0.08-10 μM
Incubation Time:

24 h (pre-incubation)
Result:

Inhibited 6-OHDA-induced expression of cleaved caspase-3, cleaved caspase-9, and cleaved PARP in a dose-dependent manner.

In Vivo

5,7-dihydroxychromone, delivered as an active component of Daphniphyllum macropodum fruit extract
(DME), dose-dependently reduces serum glucose, total cholesterol, and triacylglycerol levels, and increases liver PPARγ and LXRα mRNA expression, in streptozotocin-/high-fat diet-induced type 2 diabetic ICR mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 31721-94-5
Appearance Solid
Molecular Weight 178.14
Formula C₉H₆O₄
Color White to light yellow
SMILES

O=C1C=COC2=CC(O)=CC(O)=C12
Structure Classification
- Flavonoids
- Isoflavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

All

Sort by:

Journal Impact Factor

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Planta Medica International Open

2022; 9(01): e108-e115.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (561.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6136 mL

28.0678 mL

56.1356 mL

5 mM

1.1227 mL

5.6136 mL

11.2271 mL

10 mM

0.5614 mL

2.8068 mL

5.6136 mL

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim DW, et al. Neuroprotection against 6-OHDA-induced oxidative stress and apoptosis in SH-SY5Y cells by 5,7-Dihydroxychromone: Activation of the Nrf2/ARE pathway. Life Sci. 2015;130:25-30. [Content Brief]

[2]. Koo HJ, et al. Anti-diabetic properties of Daphniphyllum macropodum fruit and its active compound. Biosci Biotechnol Biochem. 2014;78(8):1392-401. [Content Brief]

[3]. Vaughn SF, et al. Phytotoxic and antimicrobial activity of 5,7-dihydroxychromone from peanut shells. J Chem Ecol. 1995;21(2):107-115. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.6136 mL	28.0678 mL	56.1356 mL	140.3391 mL
	5 mM	1.1227 mL	5.6136 mL	11.2271 mL	28.0678 mL
	10 mM	0.5614 mL	2.8068 mL	5.6136 mL	14.0339 mL
	15 mM	0.3742 mL	1.8712 mL	3.7424 mL	9.3559 mL
	20 mM	0.2807 mL	1.4034 mL	2.8068 mL	7.0170 mL
	25 mM	0.2245 mL	1.1227 mL	2.2454 mL	5.6136 mL
	30 mM	0.1871 mL	0.9356 mL	1.8712 mL	4.6780 mL
	40 mM	0.1403 mL	0.7017 mL	1.4034 mL	3.5085 mL
	50 mM	0.1123 mL	0.5614 mL	1.1227 mL	2.8068 mL
	60 mM	0.0936 mL	0.4678 mL	0.9356 mL	2.3390 mL
	80 mM	0.0702 mL	0.3508 mL	0.7017 mL	1.7542 mL
	100 mM	0.0561 mL	0.2807 mL	0.5614 mL	1.4034 mL

Keywords

5,7-Dihydroxychromone

2 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

5,7-Dihydroxychromone

Anti-Infection Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Anti-diabetic Compound Library

Oxygen Sensing Compound Library

Antifungal Compound Library

Ferroptosis Compound Library

Medicine Food Homology Compound Library

Phenols Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Flavonoids Library

Neuroprotective Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Pulmonary Fibrosis Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Drug Fragment Library

Publications Citing Use of MedChemExpress (MCE) 5,7-Dihydroxychromone

All Caspase Isoforms

All PARP Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

5,7-Dihydroxychromone Related Classifications

HY-N1970 Related Classifications