5-Formyl-dCTP lithium
5-Formyl-dCTP (5-Formyl-deoxycytidine) lithium is a DCTPP1 substrate. 5-Formyl-dCTP lithium acts as a mutagen/epigenetic disruptor via DNA polymerase incorporation, mutagenic mispairings, and disturbed epigenetic programming. 5-Formyl-dCTP lithium can be used for research on genotoxicity.
For research use only. We do not sell to patients.
- CAS No.: 2409156-19-8
- Formula: C10H12Li4N3O14P3
- Molecular Weight:518.90
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
5-Formyl-dCTP lithium exhibits enriched, specific binding to Con A (Kd,eff = 20 nM) with minimal binding to PSA (Kd,eff > 1 μM) after three rounds of two-color click-PD screening[1].
5-Formyl-dCTP lithium (10-250 μM) is hydrolysed by purified recombinant human DCTPP1 with high efficiency, exhibiting a Km of 9.2 μM, the lowest substrate affinity reported for the enzyme, and a specificity constant of 322,000/M·s[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2409156-19-8
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Molecular Weight 518.90
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Formula C10H12Li4N3O14P3
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SMILES
O=CC1=CN([C@@H](C[C@@H]2O)O[C@@H]2COP(OP(OP(O[Li])(O[Li])=O)(O[Li])=O)(O[Li])=O)C(N=C1N)=O
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Synonyms
5-Formyl-deoxycytidine lithium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
Purity & Documentation
References
[1]. Gordon CKL, et al. Click-Particle Display for Base-Modified Aptamer Discovery. ACS Chem Biol. 2019;14(12):2652-2662. [Content Brief]
[2]. Requena CE, et al. The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells. Biochem J. 2014;459(1):171-180. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5-Formyl-dCTP lithium Related Classifications
HY-160596 Related Classifications
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