9-cis Retinol

9-cis Retinol (2.5 µM; 5 h) achieves intracellular accumulation of approximately 160 pmol/mg protein at 5 h after treatment of LXSN/Hep G2 human hepatocellular carcinoma cells^[1].
9-cis Retinol induces ARAT and LRAT activities capable of esterifying 9-cis Retinol, as well as bile salt-dependent and bile salt-independent retinyl ester hydrolase activities, in HSC-T6 rat hepatic stellate cells and LXSN/Hep G2, LRDHSN/Hep G2 human hepatocellular carcinoma cells^[1].
9-cis Retinol potently inhibits the activity of 9-cis-retinaldehyde dehydrogenase in homogenates of LXSN/Hep G2 human hepatocellular carcinoma cells, with a concentration of 5 µM required to achieve 50% inhibition^[1].
9-cis Retinol can be oxidized to 9-cis-Retinal (HY-W009310) by cRDH expressed in LRDHSN/Hep G2 human hepatocellular carcinoma cells; the equilibrium constant of this reaction is 4.9 × 10^-9 M at 37 °C and pH 8.0, and the reaction tends to generate 9-cis-retinal^[1].
9‑cis Retinol undergoes oxidation catalyzed by cRDH expressed in LRDHSN/Hep G2 human hepatocellular carcinoma cells, with an apparent K_m of 0.44 µM and an apparent V_max of 0.126 nmol mg^‑1 min^‑1[1].
9-cis retinol (100 μM; 20 min) serves as a substrate for NAD⁺-specific recombinant RDH4 derived from baculovirus-infected Sf9 cells. This enzyme catalyzes its conversion to 9-cis-Retinal, whereas all-trans retinol is not oxidized by this enzyme^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.