1. Neuronal Signaling
  2. Amyloid-β

Aftin-4 

Cat. No.: HY-111267 Purity: 98.13%
Handling Instructions

Aftin-4 is an Amyloid-β42 (42) inducer.

For research use only. We do not sell to patients.

Aftin-4 Chemical Structure

Aftin-4 Chemical Structure

CAS No. : 866893-90-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 165 In-stock
Estimated Time of Arrival: December 31
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 240 In-stock
Estimated Time of Arrival: December 31
25 mg USD 480 In-stock
Estimated Time of Arrival: December 31
50 mg USD 850 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1500 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Aftin-4 is an Amyloid-β42 (42) inducer.

IC50 & Target

Amyloid-β[1]

In Vitro

Aftin-4 selectively and potently increases Aβ1-42 in N2a cells, primary neurons, and brain lysates, with an EC50 value around 30 μM[1].

In Vivo

Aftin-4 increases Aβ1-42 levels in vivo in mice and provokes rapidly a sustained toxicity highly reminiscent of Alzheimer’s disease (AD). Aftin-4 is administered at increasing doses, between 3 and 20 nmol/mouse, into the lateral ventricle and animals are sacrificed at various time points, between 3 to 14 days after injection. The hippocampus is dissected out the contents in Aβ1-40 or Aβ1-42 is determined using a mouse ELISA assay. Aftin-4 dose-dependently and significantly increases Aβ1-42 content, up to +216% at the highest dose tested[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 250 mg/mL (678.46 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7139 mL 13.5693 mL 27.1385 mL
5 mM 0.5428 mL 2.7139 mL 5.4277 mL
10 mM 0.2714 mL 1.3569 mL 2.7139 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[1]

Mice[1]
Male Swiss OF-1 mice, aged 7-9 weeks and weighing 32±2 g are used. Aftin-4 is solubilized in DMSO at a concentration of 3 mg/mL and stored at -20°C until use. Aftin-4 is administered intracerebroventricularly (i.c.v. ), with a Hamilton microsyringe equipped with a 3-mm needle in a final volume of 3 μL per mouse. The injection coordinates are -0.4 mm with respect to bregma, 1.00mm to the right from the central, and 2.50mm in depth. Aftin-4 is also injected intraperitoneally (i.p.). Aftin-4 is solubilized in DMSO 40% in distilled water at 2, 6 or 20 mg/mL and administered in a final volume of 100 μL/20 g body weight[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

368.48

Formula

C₂₀H₂₈N₆O

CAS No.

866893-90-5

SMILES

CC[[email protected]@H](NC1=NC(N(C)CC2=CC=CC=C2)=C3N=CN(C(C)C)C3=N1)CO

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Aftin-4
Cat. No.:
HY-111267
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Aftin-4

Cat. No.: HY-111267