CDD0102

A9 L cells expressing muscarinic receptor subtypes are cultured. Biochemical studies examine the ability of compounds (e.g., CDD0102, 0.01 nM, 0.1 nM, 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM) to stimulate relevant biochemical responses through muscarinic receptors expressed in A9 Lcells. Assays of PI metabolism provides a measure of in vitro activity associated with M₁, M₃, and M₅ receptor activation, while inhibition of adenylyl cyclase activity determined efficacy at M₂ and M₄ receptors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[1]
Young, male CF-1 mice are administered CDD0102 or xanomeline by either p.o. or i.p. injection with doses from 1 to 1,000 mg/kg by each route, and monitored for 24 h. Animals are observed daily for 1 wk for appearance of delayed toxicity. A second set of animals is treated with a narrow range of concentrations based on the preliminary studies to determine the LD₅₀.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.