1. MAPK/ERK Pathway
  2. Ribosomal S6 Kinase (RSK)
  3. CMK

CMK 

Cat. No.: HY-52101 Purity: 99.64%
Handling Instructions

CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK.

For research use only. We do not sell to patients.

CMK Chemical Structure

CMK Chemical Structure

CAS No. : 821794-90-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 142 In-stock
Estimated Time of Arrival: December 31
2 mg USD 120 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
10 mg USD 300 In-stock
Estimated Time of Arrival: December 31
25 mg USD 432 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of CMK:

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Description

CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK.

In Vitro

CMK inhibits the growth of Cdc5 (L158G) with IC50 of 36 nM, greater than 30 μM for wild type Cdc5. CMK exhibits a concentration-dependent first cell cycle mitotic arrest in the cdc5-as1 strain with an IC50 of 1.1 μM. CMK inhibition of Cdc5 (L158G) leads to a first cell cycle anaphase arrest and delay in anaphase spindle migration[1].

Molecular Weight

358.82

Formula

C₁₈H₁₉ClN₄O₂

CAS No.

821794-90-5

SMILES

NC1=C(C2=NC=N1)C(C3=CC=C(C=C3)C)=C(N2CCCO)C(CCl)=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (418.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7869 mL 13.9346 mL 27.8691 mL
5 mM 0.5574 mL 2.7869 mL 5.5738 mL
10 mM 0.2787 mL 1.3935 mL 2.7869 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.97 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.97 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

For Cdc5 kinase assays, Spc72-TAP is isolated from cell extract by pulldown with IgG sepharose or rabbit IgG coupled to M-270 epoxy dynabeads and incubated in kinase buffer (25 mM HEPES, pH 8.0, 60 mM KCl, 15 mM MnCl2, 100 μg/mL BSA, 80 nM microcystin, 1mM DTT, 100 μM 200 μCi/mL [γ-32P]ATP) in the absence or presence of 100 ng purified baculovirus expressed 6xHis-Cdc5. 32P incorporation is visualized on a Typhoon PhosphorImager, and images are processed using ImageQuant software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.64%

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CMK
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