1. Apoptosis
    Metabolic Enzyme/Protease
  2. MDM-2/p53
    E1/E2/E3 Enzyme
  3. CTX1

CTX1 

Cat. No.: HY-U00442 Purity: ≥96.0%
Handling Instructions

CTX1 is a p53 activator that overcomes HdmX-mediated p53 repression. CTX1 exhibits potent anti-cancer activity in a mouse acute myeloid leukemia (AML) model system.

For research use only. We do not sell to patients.

CTX1 Chemical Structure

CTX1 Chemical Structure

CAS No. : 501935-96-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 258 In-stock
Estimated Time of Arrival: December 31
1 mg USD 100 In-stock
Estimated Time of Arrival: December 31
5 mg USD 160 In-stock
Estimated Time of Arrival: December 31
10 mg USD 290 In-stock
Estimated Time of Arrival: December 31
25 mg USD 650 In-stock
Estimated Time of Arrival: December 31
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Description

CTX1 is a p53 activator that overcomes HdmX-mediated p53 repression. CTX1 exhibits potent anti-cancer activity in a mouse acute myeloid leukemia (AML) model system[1].

IC50 & Target

p53[1]

In Vitro

CTX1 binds directly to HdmX to prevent p53-HdmX complex formation, resulting in the rapidly induction of p53 in a DNA damage-independent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CTX1 ( (30 mg/kg; i.p.; five days a week; for 3 weeks) significantly enhances the survival of mice in AML model system[1].
CTX1 exhibited significant anti-cancer activity alone as well as in combination with nutlin-3[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

234.26

Formula

C₁₄H₁₀N₄

CAS No.
SMILES

N#CC1=C(C=CC(N)=C2)C2=NC3=CC(N)=CC=C31

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
References
Kinase Assay
[1]

Exponentially growing OCI cells cultures are treated with 3 μM CTX1, 8 μM Nutlin and or 15 μM RO-5963 for 4.5 hrs. Whole-cell extracts are generated using modified RIPA lysis buffer 25 mM Tris (pH 8.0), 100 mM NaCl, 0.5 mM EDTA, 0.50% NP-40 and complete protease mixture tablet. Protein extracts (~750 μg) are precleared and immune precipitation is performed using a kit according to the manufacturer's protocol. For the immunoprecipitation, mouse monoclonal anti-p53 and rabbit polyclonal anti-HDMX are used. Immune complexes are then collected, proteins are eluted and subjected to Western blotting with the indicated antibodies[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

6 week old female NOD/SCID/IL2Rγ−/− mice are injected into the tail vein with 5×106 cells primary human AML cells (n=5 per group). Drug treatment is started 2 days after tumor cell injection. Nutlin-3 is given by oral gavage (200 mg/kg) and CTX1 is injected i.p. (30 mg/kg) five days a week for 3 weeks. Flow cytometry is performed on bone marrow cells isolated from the mouse femur using a human specific CD45PE antibody using a cytometer to confirm AML infiltration into the bone marrow[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥96.0%

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Keywords:

CTX1CTX 1CTX-1MDM-2/p53E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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