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EC-17 disodium salt 

Cat. No.: HY-13615A
Handling Instructions

EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm.

For research use only. We do not sell to patients.

EC-17 disodium salt Chemical Structure

EC-17 disodium salt Chemical Structure

CAS No. : 910661-33-5

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  • Biological Activity

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  • Technical Information

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  • References

Description

EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm.

IC50 & Target

Folate receptor alpha[1]

In Vitro

EC-17 contains the fluorescein fluorochrome and has a spectral wavelength of 490–530 nm. EC-17 is synthesized by a folate (vitamin B9) and fluorescein isothiocyanate (FITC) conjugated through an ethylenediamine spacer to produce folate-FITC, with a molecular weight of 917 kDa. FITC is a derivative of fluorescein functionalized with an isothiocyanate reactive group. The folate-FITC conjugate forms a negatively charged fluorescent molecule that specifically targets cell-surface FRα and is subsequently internalized into the cytoplasm. The signal-to-background ratio (SBR) of EC-17 for HeLa cells range from 0.97 to 7.32 depending on the molarity and concentration of cancer cells[1].

In Vivo

The mean fluorescence signal from the animals injected with EC-17 to be 42,234 ± 12,234 au[1]. Fluorescence microscopy for folate-FITC shows a strong signal in all malignant tumors with FR-α expression and no signal in FR-α–negative malignant or benign lesions[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1163 mL 5.5813 mL 11.1626 mL
5 mM 0.2233 mL 1.1163 mL 2.2325 mL
10 mM 0.1116 mL 0.5581 mL 1.1163 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

KB, HeLa, and TC1 cells are plated on a cell culture treated 6-well plate and incubated for 16 hours. Once confluent, EC-17 is added cells. The cells are incubated and sealed in a light-protected environment for 45 minutes. Cells are then washed 3 times with PBS and plated and underwent fluorescence microscopy[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]

Mice are injected subcutaneously in the flank with 1.2×106 TC1 cells (C57BL/6 mice), 1.0×106 HeLa cells (NOD.Cg-Prkdcscid  Il2rgtm1Wjl/SzJ mice), or 1.0×106 KB cells (NOD.Cg-Prkdcscid, Il2rgtm1Wjl/SzJ mice). Once tumor volume reached approximately 300 mm3 half of the mice are injected with 0.1 mg/kg of EC-17 and the other half with 0.1 mg/kg of OTL38 via tail vein. Three hours later, the fluorescence of tumors is measured using Flocam[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

895.85

Formula

C₄₂H₃₆N₁₀NaO₁₀S+

CAS No.

910661-33-5

SMILES

S=C(NCCNC(CC[[email protected]@H](C(O)=O)NC(C1=CC=C(NCC2=CN=C3N=C(N)NC(C3=N2)=O)C=C1)=O)=O)NC4=CC5=C(C6(C7=C(OC8=C6C=CC(O)=C8)C=C(O)C=C7)OC5=O)C=C4.[Na+]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
EC-17 disodium salt
Cat. No.:
HY-13615A
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EC-17 disodium salt

Cat. No.: HY-13615A