F1063-0967
Based on 1 Customer Validation
F1063-0967 is a Dual-specificity phosphatase 26 (DUSP26) inhibitor with an IC50 of 11.62 μM.
For research use only. We do not sell to patients.
- Purity: 99.30%
- CAS No.: 613225-56-2
- Formula: C24H24N2O5S2
- Molecular Weight:484.59
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Covalent Screening Library
HY-L036
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Phosphatase Inhibitor Library
HY-L081
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Heterocyclic Compound Library
HY-L138
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Metabolic Enzyme Compound Library
HY-L146
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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Covalent Screening Library Plus
HY-L036P
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 11.62 μM (DUSP26)[1]
F1063-0967 is a Dual-specificity phosphatase 26 (DUSP26) inhibitor with an IC50 of 11.62 μM. At concentration above 0.1 μM, F1063-0967 remarkably induces IMR32 cell apoptosis. F1063-0967 induces apoptosis in IMR-32 cell line with an IC50 value of 4.13 μM. F1063-0967 has no effect on HL7702 cell line, and little inhibition effect on SH-SY5Y cell line[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 613225-56-2
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Appearance Solid
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Molecular Weight 484.59
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Formula C24H24N2O5S2
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Color Light yellow to yellow
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SMILES
OC1=C(C(O)=O)C=CC(NC(CCCCCN2C(S/C(C2=O)=C\C3=CC=C(C)C=C3)=S)=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (206.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.16 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
The IMR-32 cells are plated in 96-well plates with 1×103 cells per well. After the cells are allowed to settle for 24 h, F1063-0967 (0, 10, 1, and 0.1 μM) is added. An MTTassay is performed. For cell lines HL7702 and SH-SY5Y, the cells are allowed to settle for 24 h, F1063-0967 (10 μM) is added, then MTT assays are carried out after 72 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0636 mL | 10.3180 mL | 20.6360 mL | 51.5900 mL |
| 5 mM | 0.4127 mL | 2.0636 mL | 4.1272 mL | 10.3180 mL | |
| 10 mM | 0.2064 mL | 1.0318 mL | 2.0636 mL | 5.1590 mL | |
| 15 mM | 0.1376 mL | 0.6879 mL | 1.3757 mL | 3.4393 mL | |
| 20 mM | 0.1032 mL | 0.5159 mL | 1.0318 mL | 2.5795 mL | |
| 25 mM | 0.0825 mL | 0.4127 mL | 0.8254 mL | 2.0636 mL | |
| 30 mM | 0.0688 mL | 0.3439 mL | 0.6879 mL | 1.7197 mL | |
| 40 mM | 0.0516 mL | 0.2580 mL | 0.5159 mL | 1.2898 mL | |
| 50 mM | 0.0413 mL | 0.2064 mL | 0.4127 mL | 1.0318 mL | |
| 60 mM | 0.0344 mL | 0.1720 mL | 0.3439 mL | 0.8598 mL | |
| 80 mM | 0.0258 mL | 0.1290 mL | 0.2580 mL | 0.6449 mL | |
| 100 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5159 mL |
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