Genkwanin (Synonyms: Puddumetin)

Name: Genkwanin
Brand: MedChemExpress
SKU: HY-N0731
Rating: 5.0 (9 reviews)

Cat. No.: HY-N0731 Purity: 99.52%: Data Sheet
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Genkwanin is a major non-glycosylated flavonoid with anti-flammatory activities.

For research use only. We do not sell to patients.

CAS No. : 437-64-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 9 publication(s) in Google Scholar

Other Forms of Genkwanin:

Genkwanin (Standard) Get quote

9 Publications Citing Use of MCE Genkwanin

Histological Imaging/Staining

Flow Cytometry

Cell Proliferation/Viability Assay

: Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]; HE staining analysis of pathological damage in the heart, liver, lung, and kidney tissues of mice treated with H-GKA (20 mg/kg, i.p.).

: Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]; DYRK1A expression in lung tissues of asthmatic mice and mice after GKA (5, 10, 20 mg/kg, i.p.) treatment was examined using immunoblotting.

: Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]; Detection of ROS in lung tissues of mice by DCFH-DA with GKA (5, 10, 20 mg/kg, i.p.) treatment.

: Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]; DYRK1A expression in lung tissues of asthmatic mice and mice after GKA (5, 10, 20 mg/kg, i.p.) treatment was examined using g dual-labeling immunofluorescence.

: Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]; The cell viability of BEAS-2B cells was examined using CCK-8 assays after GKA (30 μM) treatment.

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Biological Activity
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Description

Genkwanin is a major non-glycosylated flavonoid with anti-flammatory activities.

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	> 20 μg/mL Compound: 4',5-dihydroxy-7-methoxyflavone	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 15217279]
A549	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 31784199]
Col2	ED₅₀	> 20 μg/mL Compound: genkwanin	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 17279798]
HCT-15	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 31784199]
HUVEC	ED₅₀	> 20 μg/mL Compound: genkwanin	Cytotoxicity against human HUVEC cells Cytotoxicity against human HUVEC cells	[PMID: 17279798]
HeLa	IC₅₀	8.1 μM Compound: Genkwanin	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784199]
KB	IC₅₀	16.4 μg/mL Compound: 7, Genkwanin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8759170]
KB	IC₅₀	8.7 μg/mL Compound: 6, genkwanin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8759170]
LNCaP	ED₅₀	> 20 μg/mL Compound: genkwanin	Cytotoxicity against human LNCaP cells Cytotoxicity against human LNCaP cells	[PMID: 17279798]
Lu1	ED₅₀	> 20 μg/mL Compound: genkwanin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 17279798]
MCF-10A	IC₅₀	75 μM Compound: Genkwanin	Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MCF7	IC₅₀	10.3 μM Compound: Genkwanin	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784199]
MDA-MB-468	IC₅₀	1.6 μM Compound: Genkwanin	Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MOLM-13	IC₅₀	> 10 μM Compound: 16	Cytotoxicity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026809]
MV4-11	IC₅₀	> 10 μM Compound: 16	Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026809]
P388	IC₅₀	10 μg/mL Compound: 6, genkwanin	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 8759170]
RAW264.7	IC₅₀	5.64 μM Compound: 14	Anti-inflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NO production incubated for 24 hrs by Griess reagent based assay Anti-inflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NO production incubated for 24 hrs by Griess reagent based assay	[PMID: 37683361]
SK-MEL-2	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 31784199]
SK-OV-3	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 31784199]
TERT-RPE1	ED₅₀	3.9 μg/mL Compound: genkwanin	Cytotoxicity against human TERT-RPE1 cells Cytotoxicity against human TERT-RPE1 cells	[PMID: 17279798]
XF498	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human XF498 cells by SRB assay Cytotoxicity against human XF498 cells by SRB assay	[PMID: 31784199]

In Vitro

Cell viability analysis shows that Genkwanin does not affect the cell viability up to a concentration of 50 μM. Genkwanin inhibits the LPS-induced production of NO in a concentration-dependent manner. iNOS only expresses in the present of external stimulus. Genkwanin could not significantly affect the activity of iNOS. The effect of Genkwanin on the production of proinflammatory cytokines is examined. Genkwanin suppresses the productions of TNF-a, IL-1b and IL-6 in LPS stimulated RAW264.7 macrophages in a concentration-dependent manner. Genkwanin significantly suppresses the AP-1 signaling pathway but has little effect on the NF-kB signaling pathway. It is indicated that Genkwanin suppresses the phosphorylation of p38 and JNK in a concentration-dependent manner, but little affects ERK1/2 phosphorylation. Originally identified as an immediate early gene, MKP-1 is then found to be a dual specificity phosphatase acting as a negative regulator of ERK1/2, JNK and p38 MAPK activities, with predominant effects on the latter two. Pretreatment with Genkwanin markedly up-regulates the expression of MKP-1 without affecting MKP-1 mRNA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

284.26

Formula

C₁₆H₁₂O₅

CAS No.

437-64-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(OC)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 31.25 mg/mL (109.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5179 mL	17.5895 mL	35.1791 mL
5 mM	0.7036 mL	3.5179 mL	7.0358 mL
10 mM	0.3518 mL	1.7590 mL	3.5179 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.76 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.5 mg/mL (1.76 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.76 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.52%

Select Batch:

Data Sheet (273 KB) SDS (394 KB)

COA (247 KB) HNMR (252 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Gao Y et al. Genkwanin inhibits proinflammatory mediators mainly through the regulation of miR-101/MKP-1/MAPK pathway in LPS-activated macrophages. PLoS One. 2014 May 6;9(5):e96741. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5179 mL	17.5895 mL	35.1791 mL	87.9477 mL
	5 mM	0.7036 mL	3.5179 mL	7.0358 mL	17.5895 mL
	10 mM	0.3518 mL	1.7590 mL	3.5179 mL	8.7948 mL
	15 mM	0.2345 mL	1.1726 mL	2.3453 mL	5.8632 mL
	20 mM	0.1759 mL	0.8795 mL	1.7590 mL	4.3974 mL
	25 mM	0.1407 mL	0.7036 mL	1.4072 mL	3.5179 mL
	30 mM	0.1173 mL	0.5863 mL	1.1726 mL	2.9316 mL
	40 mM	0.0879 mL	0.4397 mL	0.8795 mL	2.1987 mL
	50 mM	0.0704 mL	0.3518 mL	0.7036 mL	1.7590 mL
	60 mM	0.0586 mL	0.2932 mL	0.5863 mL	1.4658 mL
	80 mM	0.0440 mL	0.2199 mL	0.4397 mL	1.0993 mL
	100 mM	0.0352 mL	0.1759 mL	0.3518 mL	0.8795 mL

Genkwanin Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Genkwanin437-64-9PuddumetinVirus ProteaseInhibitorinhibitorinhibit

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Genkwanin (Synonyms: Puddumetin)

Customer Review

Other Forms of Genkwanin:

Genkwanin Related Antibodies

9 Publications Citing Use of MCE Genkwanin

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•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

Genkwanin Related Antibodies

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Complete Stock Solution Preparation Table

Genkwanin Related Classifications

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