2. Antibody-drug Conjugate/ADC Related
  3. Drug-Linker Conjugates for ADC
  4. Lys-SMCC-DM1

Lys-SMCC-DM1  (Synonyms: Lys-Nε-MCC-DM1)

Cat. No.: HY-101982 Purity: 99.61%
COA Handling Instructions

Molarity Calculator

Lys-SMCC-DM1 (Lys-Nε-MCC-DM1) is a agent-linker conjugates for ADC that can inhibit tubulin polymerization. Lys-SMCC-DM1 is the active metabolite of T-DM1. T-DM1 is a HER2-targeting ADC with a tubulin polymerization inhibitor DM1. Lys-SMCC-DM1 can be used in the research of breast cancer.

For research use only. We do not sell to patients.

Lys-SMCC-DM1 Chemical Structure

Lys-SMCC-DM1 Chemical Structure

CAS No. : 1281816-04-3

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5 mg USD 880 In-stock
10 mg USD 1300 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lys-SMCC-DM1:

Lys-SMCC-DM1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Lys-SMCC-DM1

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Lys-SMCC-DM1 (Lys-Nε-MCC-DM1) is a agent-linker conjugates for ADC that can inhibit tubulin polymerization. Lys-SMCC-DM1 is the active metabolite of T-DM1. T-DM1 is a HER2-targeting ADC with a tubulin polymerization inhibitor DM1. Lys-SMCC-DM1 can be used in the research of breast cancer[1][2].

IC50 & Target

Maytansinoids

 

In Vitro

Lys-SMCC-DM1 possess a low level of permeability[1].
Lys-SMCC-DM1 shows cytotoxicity in KPL-4 and MDA-MB-468 cells, with IC50 of 24.8 nM and 40.5 nM respectively[2].
Lys-SMCC-DM1 (200 μM) inhibits the SLC46A3-mediated uptake of estrone 3-sulfate in MDCKII/SLC46A3 dC cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lys-SMCC-DM1 Related Antibodies
Molecular Weight

1103.71

Formula

C53H75ClN6O15S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]1([C@@](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)([H])OC([C@H](C)N(C)C(CCS[C@@H](CC4=O)C(N4C[C@@H]5CC[C@@H](C(NCCCC[C@H](N)C(O)=O)=O)CC5)=O)=O)=O)[C@H]([C@@H]([C@](OC6=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N6)O)C)O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (90.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9060 mL 4.5302 mL 9.0604 mL
5 mM 0.1812 mL 0.9060 mL 1.8121 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.61%

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9060 mL 4.5302 mL 9.0604 mL 22.6509 mL
5 mM 0.1812 mL 0.9060 mL 1.8121 mL 4.5302 mL
10 mM 0.0906 mL 0.4530 mL 0.9060 mL 2.2651 mL
15 mM 0.0604 mL 0.3020 mL 0.6040 mL 1.5101 mL
20 mM 0.0453 mL 0.2265 mL 0.4530 mL 1.1325 mL
25 mM 0.0362 mL 0.1812 mL 0.3624 mL 0.9060 mL
30 mM 0.0302 mL 0.1510 mL 0.3020 mL 0.7550 mL
40 mM 0.0227 mL 0.1133 mL 0.2265 mL 0.5663 mL
50 mM 0.0181 mL 0.0906 mL 0.1812 mL 0.4530 mL
60 mM 0.0151 mL 0.0755 mL 0.1510 mL 0.3775 mL
80 mM 0.0113 mL 0.0566 mL 0.1133 mL 0.2831 mL
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Lys-SMCC-DM1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lys-SMCC-DM11281816-04-3Lys-Nε-MCC-DM1Drug-Linker Conjugates for ADClinker-payloadtubulin polymerizationHER2growth inhibitoryBreast cancerKPL-4MDA-MB-468Inhibitorinhibitorinhibit

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