1. Cell Cycle/DNA Damage
  2. p97
  3. NMS-859

NMS-859 

Cat. No.: HY-15714 Purity: 97.65%
Handling Instructions

NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

For research use only. We do not sell to patients.

NMS-859 Chemical Structure

NMS-859 Chemical Structure

CAS No. : 1449236-96-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 462 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2520 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Description

NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

IC50 & Target

IC50: 360 nM (Cellular p97, 1 mM ATP), 370 nM (Cellular p97, 60 μM ATP)[1]

In Vitro

NMS-859 is a potent VCP inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively. NMS-859 shows very weak inhibitory activity against VCPC522T. NMS-859 also suppresses the proliferation of cells, with IC50s of 3.5 μM and 3.0 μM in HCT116 and HeLa cell lines, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

349.79

Formula

C₁₅H₁₂ClN₃O₃S

CAS No.

1449236-96-7

SMILES

O=S1(C2=CC=CC=C2C(NC3=CC(NC(CCl)=O)=CC=C3)=N1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 42 mg/mL (120.07 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8589 mL 14.2943 mL 28.5886 mL
5 mM 0.5718 mL 2.8589 mL 5.7177 mL
10 mM 0.2859 mL 1.4294 mL 2.8589 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO2 atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC50 values are calculated using the percentage of growth of treated cells versus the untreated control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

NMS-859NMS859NMS 859p97VCPCdc48Inhibitorinhibitorinhibit

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HY-15714
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