Pulchinenoside C (Synonyms: Anemoside B4)

Name: Pulchinenoside C
Brand: MedChemExpress
SKU: HY-N0205
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0205 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Pulchinenoside C (Anemoside B4) is Pulsatilla koreana Nakai that have many numerous biological effects in vitro, including enhancing hypoglycemic, anti-tumor, neuroprotective and anti-angiogenic activity.

For research use only. We do not sell to patients.

CAS No. : 129741-57-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Pulchinenoside C:

Pulchinenoside A In-stock
Pulchinenoside C (Standard) Get quote

5 Publications Citing Use of MCE Pulchinenoside C

Cell Proliferation/Viability Assay

RT-PCR

ELISA

: Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]; Effects of Pulchinenoside C (PC) (1, 5, 10, 20, 50, 100, 200 μM) on the ADTC5 cells were determined in response to increasing concentrations for 24, 48 and 72 h.

: Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]; The expression levels of iNOS and COX‐2 mRNA were measured using qRT‐PCR treated with Pulchinenoside C (PC) (1, 5 μM).

: Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]; The expression of IL‐6 and TNF‐α in the ADTC5 cell supernatants was detected using ELISA treated with Pulchinenoside C (PC) (1, 5 μM).

: Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]; The expression levels of iNOS and COX2 after treatment with different concentrations of Pulchinenoside C (PC) (1, 5 μM) were detected using western blotting.

: Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]; Typical collagen‐II was detected by immunofluorescence combined with nuclear DAPI staining treated with Pulchinenoside C (PC) (1, 5 μM).

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
THP-1	IC₅₀	>= 80 μM Compound: AB4	Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay	[PMID: 38687956]

In Vitro

Pulchinenoside C (Anemoside B4) can inhibit the secretion of IL-10; SSA, SSD and PNS up-regulated IL-2 expression^[1]. Pulchinenoside C (Anemoside B4), with IC50 value more than 390 ug/mL. Anemoside B4 inhibits cell proliferation^[2]. Pulchinenoside C (Anemoside B4) can significantly suppress the secretion of the inflammatory factor E-selectin by endothelial cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1221.38

Formula

C₅₉H₉₆O₂₆

CAS No.

129741-57-7

Appearance

Solid

Color

White to light yellow

SMILES

O=C([C@]1(CC[C@H]2C(C)=C)[C@@]2([H])[C@](CC[C@@]3([H])[C@]4(CC[C@]5([H])[C@@]3(CC[C@H](O[C@@](OC[C@H](O)[C@@H]6O)([H])[C@@H]6O[C@@](O[C@@H](C)[C@H](O)[C@H]7O)([H])[C@@H]7O)[C@@]5(C)CO)C)C)([H])[C@@]4(C)CC1)O[C@@H]([C@@H]([C@@H](O)[C@@H]8O)O)O[C@@H]8CO[C@@H]([C@@H]([C@@H](O)[C@@H]9O[C@@](O[C@@H](C)[C@H](O)[C@H]%10O)([H])[C@@H]%10O)O)O[C@@H]9CO

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (81.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (40.94 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8187 mL	4.0937 mL	8.1875 mL
5 mM	0.1637 mL	0.8187 mL	1.6375 mL
10 mM	0.0819 mL	0.4094 mL	0.8187 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (261 KB) HNMR (348 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. Hu Y et al. Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells. J Immunotoxicol. 2016;13(2):141-7. [Content Brief]

[2]. Liu M et al. Cytotoxicity of the compounds isolated from Pulsatilla chinensis saponins and apoptosis induced by 23-hydroxybetulinic acid. Pharm Biol. 2015 Jan;53(1):1-9. [Content Brief]

[3]. Hu Y et al. Chinese herbal medicinal ingredients inhibit secretion of IL-6, IL-8, E-selectin and TXB2 in LPS-induced rat intestinal microvascular endothelial cells. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.8187 mL	4.0937 mL	8.1875 mL	20.4687 mL
	5 mM	0.1637 mL	0.8187 mL	1.6375 mL	4.0937 mL
	10 mM	0.0819 mL	0.4094 mL	0.8187 mL	2.0469 mL
	15 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3646 mL
	20 mM	0.0409 mL	0.2047 mL	0.4094 mL	1.0234 mL
	25 mM	0.0327 mL	0.1637 mL	0.3275 mL	0.8187 mL
	30 mM	0.0273 mL	0.1365 mL	0.2729 mL	0.6823 mL
	40 mM	0.0205 mL	0.1023 mL	0.2047 mL	0.5117 mL
DMSO	50 mM	0.0164 mL	0.0819 mL	0.1637 mL	0.4094 mL
	60 mM	0.0136 mL	0.0682 mL	0.1365 mL	0.3411 mL
	80 mM	0.0102 mL	0.0512 mL	0.1023 mL	0.2559 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pulchinenoside C Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pulchinenoside C129741-57-7Anemoside B4Anemoside B 4Anemoside B-4OthersInhibitorinhibitorinhibit

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Pulchinenoside C (Synonyms: Anemoside B4)

Customer Review

Other Forms of Pulchinenoside C:

Pulchinenoside C Related Antibodies

5 Publications Citing Use of MCE Pulchinenoside C

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Pulchinenoside C Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Pulchinenoside C Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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