Pulchinenoside C
Based on 6 publication(s) in Google Scholar
Pulchinenoside C (Anemoside B4) is Pulsatilla koreana Nakai that have many numerous biological effects in vitro, including enhancing hypoglycemic, anti-tumor, neuroprotective and anti-angiogenic activity.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 129741-57-7
- Formula: C59H96O26
- Molecular Weight:1221.38
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Pulchinenoside C
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Cell Proliferation/Viability Assay
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RT-PCR
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ELISA
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WB
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IF
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | IC50 |
>=80 μM
Compound: AB4
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Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay
Cytotoxicity against human THP-1 cells assessed as inhibition of cell viability incubated for 24 hrs by CCK8 assay
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[PMID: 38687956] |
Pulchinenoside C (Anemoside B4) can inhibit the secretion of IL-10; SSA, SSD and PNS up-regulated IL-2 expression[1]. Pulchinenoside C (Anemoside B4), with IC50 value more than 390 ug/mL. Anemoside B4 inhibits cell proliferation[2]. Pulchinenoside C (Anemoside B4) can significantly suppress the secretion of the inflammatory factor E-selectin by endothelial cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 129741-57-7
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Appearance Solid
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Molecular Weight 1221.38
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Formula C59H96O26
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Color White to light yellow
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SMILES
O=C([C@]1(CC[C@H]2C(C)=C)[C@@]2([H])[C@](CC[C@@]3([H])[C@]4(CC[C@]5([H])[C@@]3(CC[C@H](O[C@@](OC[C@H](O)[C@@H]6O)([H])[C@@H]6O[C@@](O[C@@H](C)[C@H](O)[C@H]7O)([H])[C@@H]7O)[C@@]5(C)CO)C)C)([H])[C@@]4(C)CC1)O[C@@H]([C@@H]([C@@H](O)[C@@H]8O)O)O[C@@H]8CO[C@@H]([C@@H]([C@@H](O)[C@@H]9O[C@@](O[C@@H](C)[C@H](O)[C@H]%10O)([H])[C@@H]%10O)O)O[C@@H]9CO
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Synonyms
Anemoside B4
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Free Radic Biol Med
The MafG/Bach1-Lcn2 transcriptional axis drives ferroptosis in sepsis-induced acute lung injury via disrupting redox homeostasis. [Abstract]2025 Dec 29:245:250-268. PMID: 41475687 -
Int J Mol Sci
Cycloastragenol Improves Fatty Acid Metabolism Through NHR-49/FAT-7 Suppression and Potent AAK-2 Activation in Caenorhabditis elegans Obesity Model. [Abstract]2026 Jan 13;27(2):772. PMID: 41596421 -
Mol Cell Biochem
Anemoside B4 inhibits mitochondrial dysfunction-related mitophagy in asthma by regulating KAT2B/IRF3 axis. [Abstract]2026 Jun 2. PMID: 42228271 -
J Cell Mol Med
Pulchinenoside C Attenuates the Development of Osteoarthritis by Inhibiting the PI3K/AKT/NF-κB Signalling Pathway. [Abstract]2025 Aug;29(15):e70738. PMID: 40746264
Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]
Effects of Pulchinenoside C (PC) (1, 5, 10, 20, 50, 100, 200 μM) on the ADTC5 cells were determined in response to increasing concentrations for 24, 48 and 72 h.
Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]
The expression levels of iNOS and COX‐2 mRNA were measured using qRT‐PCR treated with Pulchinenoside C (PC) (1, 5 μM).
Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]
The expression of IL‐6 and TNF‐α in the ADTC5 cell supernatants was detected using ELISA treated with Pulchinenoside C (PC) (1, 5 μM).
Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]
The expression levels of iNOS and COX2 after treatment with different concentrations of Pulchinenoside C (PC) (1, 5 μM) were detected using western blotting.
Pulchinenoside C purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2025 Aug;29(15):e70738. [Abstract]
Typical collagen‐II was detected by immunofluorescence combined with nuclear DAPI staining treated with Pulchinenoside C (PC) (1, 5 μM).
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Solvent & Solubility
DMSO : ≥ 100 mg/mL (81.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (40.94 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hu Y et al. Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells. J Immunotoxicol. 2016;13(2):141-7. [Content Brief]
[2]. Liu M et al. Cytotoxicity of the compounds isolated from Pulsatilla chinensis saponins and apoptosis induced by 23-hydroxybetulinic acid. Pharm Biol. 2015 Jan;53(1):1-9. [Content Brief]
[3]. Hu Y et al. Chinese herbal medicinal ingredients inhibit secretion of IL-6, IL-8, E-selectin and TXB2 in LPS-induced rat intestinal microvascular endothelial cells. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.8187 mL | 4.0937 mL | 8.1875 mL | 20.4687 mL |
| 5 mM | 0.1637 mL | 0.8187 mL | 1.6375 mL | 4.0937 mL | |
| 10 mM | 0.0819 mL | 0.4094 mL | 0.8187 mL | 2.0469 mL | |
| 15 mM | 0.0546 mL | 0.2729 mL | 0.5458 mL | 1.3646 mL | |
| 20 mM | 0.0409 mL | 0.2047 mL | 0.4094 mL | 1.0234 mL | |
| 25 mM | 0.0327 mL | 0.1637 mL | 0.3275 mL | 0.8187 mL | |
| 30 mM | 0.0273 mL | 0.1365 mL | 0.2729 mL | 0.6823 mL | |
| 40 mM | 0.0205 mL | 0.1023 mL | 0.2047 mL | 0.5117 mL | |
| DMSO | 50 mM | 0.0164 mL | 0.0819 mL | 0.1637 mL | 0.4094 mL |
| 60 mM | 0.0136 mL | 0.0682 mL | 0.1365 mL | 0.3411 mL | |
| 80 mM | 0.0102 mL | 0.0512 mL | 0.1023 mL | 0.2559 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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