SB 204990

Name: SB 204990
Brand: MedChemExpress
SKU: HY-16450
Rating: 5.0 (25 reviews)

Cat. No.: HY-16450 Purity: 99.75%: Data Sheet
COA

SDS
Handling Instructions
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SB 204990 is a potent and specific inhibitor of ATP citrate lyase (ACLY) enzyme.

For research use only. We do not sell to patients.

CAS No. : 154566-12-8

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 25 publication(s) in Google Scholar

25 Publications Citing Use of MCE SB 204990

Bio/Physico-chemical Assay

Flow Cytometry

RT-PCR

: SB 204990 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8098):1022-1031. [Abstract]; SB 204990 (SB, 100 μM, 16 h) reduced cytosolic AcCoA levels in HeLa-tet-on-mt-Keima cells.

: SB 204990 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8098):1022-1031. [Abstract]; SB 204990 (SB, 100 μM, 16 h) enhanced mitophagy, as indicated by an increase in the acidic mt-Keima signal in HeLa-tet-on-mt-Keima cells.

: SB 204990 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8098):1022-1031. [Abstract]; SB 204990 (SB, 100 μM, 16 h) increased LC3 expression and decreased TIM23 and MT-CO2 levels in HeLa-tet-on-mt-Keima cells.

: SB 204990 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8098):1022-1031. [Abstract]; SB 204990 (SB, 100 μM, 16 h) increased the acidic mt-Keima signal in MCF7 and A549 cells.

: SB 204990 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8098):1022-1031. [Abstract]; SB 204990 (SB, 100 μM, 16 h) decreased mitochondrial mass in MCF7, A549, and HeLa cells.

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Biological Activity
Purity & Documentation
References
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Description

SB 204990 is a potent and specific inhibitor of ATP citrate lyase (ACLY) enzyme.

IC₅₀ & Target

ACLY^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	24 μM Compound: 16; SB-204990	Antiproliferative activity against human A549 cells Antiproliferative activity against human A549 cells	[PMID: 30195238]
PC-3	IC₅₀	26 μM Compound: 16; SB-204990	Antiproliferative activity against human PC3 cells Antiproliferative activity against human PC3 cells	[PMID: 30195238]
SK-OV-3	IC₅₀	53 μM Compound: 16; SB-204990	Antiproliferative activity against human SKOV3 cells Antiproliferative activity against human SKOV3 cells	[PMID: 30195238]

In Vitro

SB204990 (SB) is a specific inhibitor of ACLY enzyme. SB204990 treatment leads to a decrease in cytosolic Ac-CoA level and is thus expected to decrease acetylated and active β-catenin levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SB 204990, when administered orally to rats, is absorbed into the systemic circulation. When administered in the diet (0.05-0.25%, w/w) for 1 week, SB 204990 causes a dose-related decrease in plasma cholesterol (by up to 46%) and triglyceride levels (by up to 80%) in rats. SB 204990 (25 mg/kg per day) also decreases plasma cholesterol levels (by up to 23%) and triglyceride levels (by up to 38%) in the dog, preferentially decreasing low-density lipoprotein compared with high-density lipoprotein cholesterol levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

389.27

Formula

C₁₈H₂₂Cl₂O₅

CAS No.

154566-12-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C[C@]1(O)C(O[C@H](CCCCCCC2=CC=C(Cl)C=C2Cl)C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (256.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (64.22 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5689 mL	12.8446 mL	25.6891 mL
5 mM	0.5138 mL	2.5689 mL	5.1378 mL
10 mM	0.2569 mL	1.2845 mL	2.5689 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2.56 mg/mL (6.58 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) HNMR (473 KB) RP-HPLC (265 KB) LCMS (92 KB) Elemental Analysis Report (103 KB)

Handling Instructions (2659 KB)

References

[1]. Shares BH, et al. Active mitochondria support osteogenic differentiation by stimulating β-catenin acetylation. J Biol Chem. 2018 Oct 12;293(41):16019-16027. [Content Brief]

[2]. Pearce NJ, et al. The role of ATP citrate-lyase in the metabolic regulation of plasma lipids. Hypolipidaemic effects of SB-204990, a lactone prodrug of the potent ATP citrate-lyase inhibitor SB-201076. Biochem J. 1998 Aug 15;334 ( Pt 1):113-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.5689 mL	12.8446 mL	25.6891 mL	64.2228 mL
	5 mM	0.5138 mL	2.5689 mL	5.1378 mL	12.8446 mL
	10 mM	0.2569 mL	1.2845 mL	2.5689 mL	6.4223 mL
	15 mM	0.1713 mL	0.8563 mL	1.7126 mL	4.2815 mL
	20 mM	0.1284 mL	0.6422 mL	1.2845 mL	3.2111 mL
	25 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5689 mL
	30 mM	0.0856 mL	0.4282 mL	0.8563 mL	2.1408 mL
	40 mM	0.0642 mL	0.3211 mL	0.6422 mL	1.6056 mL
	50 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2845 mL
	60 mM	0.0428 mL	0.2141 mL	0.4282 mL	1.0704 mL
DMSO	80 mM	0.0321 mL	0.1606 mL	0.3211 mL	0.8028 mL
DMSO	100 mM	0.0257 mL	0.1284 mL	0.2569 mL	0.6422 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

SB 204990 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SB 204990154566-12-8SB204990SB-204990ATP Citrate LyaseACLYInhibitorinhibitorinhibit

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SB 204990

Customer Review

SB 204990 Related Antibodies

25 Publications Citing Use of MCE SB 204990

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Complete Stock Solution Preparation Table

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