SMCC-DM1 (Synonyms: DM1-SMCC)

Name: SMCC-DM1
Brand: MedChemExpress
SKU: HY-101070
Rating: 5.0 (12 reviews)

Cat. No.: HY-101070 Purity: 99.23%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SMCC-DM1 (DM1-SMCC) is a agent-linker conjugate composed of a potent microtubule-disrupting agent DM1 and a linker SMCC to make antibody agent conjugate (ADC).

For research use only. We do not sell to patients.

CAS No. : 1228105-51-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 12 publication(s) in Google Scholar

12 Publications Citing Use of MCE SMCC-DM1

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

: SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Mol Ther. 2022 Jul 6;30(7):2522-2536. [Abstract]; ScFab Pigbak#2 and SMCC-DM1 were mixed in a molar ratio of 1:10 in PBS (pH 7.5) at 4 ℃ overnight. B16F10 cells were incubated with Pigbak#2-DM1 (0.05-50 nM) at various concentrations.

: SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.; SMCC-DM1 (10 mM). Dose-response analysis of the effect of cetuximab-DM1 (1-100000 nM; 120 h) on the proliferation of parental HCC1954 and HCC-TDM1R cells. IC₅₀ values were represented in the graph and detailed in the table.

: SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.; SMCC-DM1 (10 mM). In vivo effect of cetuximab-DM1 (i.p.; 15 mg/kg) on tumor growth in BALB/c nu/nu mice injected with HCC1954, HCC-TDM1R#19, or HCCTDM1R#20 cells.

: SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.; SMCC-DM1 (10 mM). Colocalization (yellow) between cetuximab-DM1 (cet-DM1) (30 min-24 h; 10 nM) and LAMP1 occurred at the indicated incubation times in HCC1954 and HCC-TDM1R cells. Cetuximab-DM1 staining red, LAMP1: green, DAPI: blue.

: SMCC-DM1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2023 Feb 1:554:216024.; SMCC-DM1 (10 mM). Western blot analyses showed the effect of cetuximab-DM1 (10 nM; 1-3 d) on the EGFR levels in HCC1954 and HCC-TDM1R cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

SMCC-DM1 (DM1-SMCC) is a agent-linker conjugate composed of a potent microtubule-disrupting agent DM1 and a linker SMCC to make antibody agent conjugate (ADC)^[1].

IC₅₀ & Target

Maytansinoids

In Vitro

SMCC-DM1 inhibits HCC1954 and MDA-MB-468 cells proliferation with IC₅₀s of 17.2 and 49.9 nM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1072.61

Formula

C₅₁H₆₆ClN₅O₁₆S

CAS No.

1228105-51-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@H]([C@@]1(C)[C@H]([C@@H]([C@@]2([H])OC(N[C@]3(O)C2)=O)C)O1)CC(N(C)C4=CC(C/C(C)=C/C=C/[C@H]3OC)=CC(OC)=C4Cl)=O)[C@@H](N(C(CCSC(C5=O)CC(N5CC6CCC(C(ON7C(CCC7=O)=O)=O)CC6)=O)=O)C)C

Shipping

Shipping with dry ice.

Storage

-80°C, stored under nitrogen

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (15.54 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9323 mL	4.6615 mL	9.3231 mL
5 mM	0.1865 mL	0.9323 mL	1.8646 mL
10 mM	0.0932 mL	0.4662 mL	0.9323 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (1.56 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.67 mg/mL (1.56 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (1.56 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.23%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (258 KB) HNMR (451 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Shao S, et al. Site-specific and hydrophilic ADCs through disulfide-bridged linker and branched PEG. Bioorg Med Chem Lett. 2018 May 1;28(8):1363-1370. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9323 mL	4.6615 mL	9.3231 mL	23.3076 mL
	5 mM	0.1865 mL	0.9323 mL	1.8646 mL	4.6615 mL
	10 mM	0.0932 mL	0.4662 mL	0.9323 mL	2.3308 mL
	15 mM	0.0622 mL	0.3108 mL	0.6215 mL	1.5538 mL

SMCC-DM1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMCC-DM11228105-51-8DM1-SMCCDrug-Linker Conjugates for ADCInhibitorinhibitorinhibit

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SMCC-DM1 (Synonyms: DM1-SMCC)

Customer Review

SMCC-DM1 Related Antibodies

12 Publications Citing Use of MCE SMCC-DM1

Featured Recommendations

•Related Small Molecules:

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View All Drug-Linker Conjugates for ADC Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

SMCC-DM1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SMCC-DM1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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