Thiamine pyrophosphate

Name: Thiamine pyrophosphate
Brand: MedChemExpress
SKU: HY-113076
Rating: 4.5 (1 reviews)

Cat. No.: HY-113076 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Thiamine pyrophosphate is the coenzyme form of Vitamin B1, and is a required intermediate in the pyruvate dehydrogenase complex and the ketoglutarate dehydrogenase complex. Thiamine pyrophosphate is necessary for oxidative phosphorylation and the pentose phosphate pathway by acting as a cofactor for α-ketoacid dehydrogenases.

For research use only. We do not sell to patients.

Thiamine pyrophosphate Chemical Structure

CAS No. : 154-87-0

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine pyrophosphate:

Thiamine pyrophosphate-d₃ Get quote

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•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vivo

Thiamine pyrophosphate (25 mg/kg, i.p.) inhibits the ischemia-reperfusion induced oxidative damage in rat ovarian tissue^[1].
Thiamine pyrophosphate (20 mg/kg, i.p., once a day for 14 days) prevents Cisplatin (HY-17394) induced cardiotoxicity, and associated oxidative stress and heart injury in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

460.77

Formula

C₁₂H₁₉ClN₄O₇P₂S

CAS No.

154-87-0

Appearance

Solid

Color

White to off-white

SMILES

OP(OP(OCCC1=C(C)[N+](CC2=CN=C(C)N=C2N)=CS1)(O)=O)(O)=O.[Cl-]

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 41.67 mg/mL (90.44 mM; Need ultrasonic)

DMSO : 1 mg/mL (2.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1703 mL	10.8514 mL	21.7028 mL
5 mM	0.4341 mL	2.1703 mL	4.3406 mL
10 mM	0.2170 mL	1.0851 mL	2.1703 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (108.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (284 KB) SDS (0 KB)

COA (250 KB) RP-HPLC (232 KB)

Handling Instructions (2659 KB)

References

[1]. Demiryilmaz I, et al. A comparative investigation of biochemical and histopathological effects of thiamine and thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat ovarian tissue. Arch Pharm Res. 2013 Sep;36(9):1133-9. [Content Brief]

[2]. Coskun R, et al. The protective effect of thiamine pyrophosphate, but not thiamine, against cardiotoxicity induced with cisplatin in rats. Drug Chem Toxicol. 2014 Jul;37(3):290-4. [Content Brief]

[3]. de Jong L, et al. Thiamine pyrophosphate biosynthesis and transport in the nematode Caenorhabditis elegans. Genetics. 2004 Oct;168(2):845-54. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.1703 mL	10.8514 mL	21.7028 mL	54.2570 mL
H₂O	5 mM	0.4341 mL	2.1703 mL	4.3406 mL	10.8514 mL
	10 mM	0.2170 mL	1.0851 mL	2.1703 mL	5.4257 mL
	15 mM	0.1447 mL	0.7234 mL	1.4469 mL	3.6171 mL
	20 mM	0.1085 mL	0.5426 mL	1.0851 mL	2.7129 mL
	25 mM	0.0868 mL	0.4341 mL	0.8681 mL	2.1703 mL
	30 mM	0.0723 mL	0.3617 mL	0.7234 mL	1.8086 mL
	40 mM	0.0543 mL	0.2713 mL	0.5426 mL	1.3564 mL
	50 mM	0.0434 mL	0.2170 mL	0.4341 mL	1.0851 mL
	60 mM	0.0362 mL	0.1809 mL	0.3617 mL	0.9043 mL
	80 mM	0.0271 mL	0.1356 mL	0.2713 mL	0.6782 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.