2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Thiamine pyrophosphate

Thiamine pyrophosphate  (Synonyms: Cocarboxylase)

Cat. No.: HY-113076 Purity: 99.93%
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Thiamine pyrophosphate is the coenzyme form of Vitamin B1, and is a required intermediate in the pyruvate dehydrogenase complex and the ketoglutarate dehydrogenase complex. Thiamine pyrophosphate is necessary for oxidative phosphorylation and the pentose phosphate pathway by acting as a cofactor for α-ketoacid dehydrogenases.

For research use only. We do not sell to patients.

CAS No. : 154-87-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
500 mg In-stock
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5 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Thiamine pyrophosphate:

Thiamine pyrophosphate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Thiamine pyrophosphate

Cell Imaging/Staining
WB
Histological Imaging/Staining
IF
In Vivo Efficacy Study

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 21.84 μg/mL) significantly decreased the relative amount of Dil-OxLDL phagocytozed in RAW264.7 cells.

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 21.84 μg/mL) significantly decreased the protein expression of MSR1 and phosphorylated PI3K p55 to PI3K p85 and the ratio of phosphorylated AKT to AKT in RAW264.7 cells.

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 60 μg/g; IV; twice a week for 4 weeks) decreased relative plaque areas in the carotid arteries and the Oil Red O-positive plaque areas in Western diet-fed ApoE−/− mice.

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 60 μg/g; IV; twice a week for 4 weeks) decreased the relative intensities of F4/80 and relative number of macrophages in Western diet-fed ApoE−/− mice.(A) Representative images of immunofluorescence staining of macrophage-associated marker F4/80 and smooth muscle cell-associated marker α-SMA.(B)Relative intensity of F4/80 in plaques of the carotid arteries. (C) Relative number of macrophages in plaques of the carotid arteries.

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 60 μg/g; IV; twice a week for 4 weeks) showed the concentration of TPP in serum of TPP-treated mice was significantly higher than that in NS-treated, ZIF-8-treated and ZIF-8@TPP-treated micein; no significant difference between all groups with regard to concentrations of alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (T-BIL), urea and creatinine (CRE) in serum in Western diet-fed ApoE−/− mice.

    Thiamine pyrophosphate purchased from MedChemExpress. Usage Cited in: Nanoscale. 2025 Feb 6;17(6):3071-3085.  [Abstract]

    Thiamine pyrophosphate (TPP; 60 μg/g; IV; twice a week for 4 weeks) showed no obvious pathological changes (e.g., necrosis, fibrosis and atrophy) were observed in vital organs (heart, liver, spleen, lungs and kidneys) in Western diet-fed ApoE−/− mice.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Thiamine pyrophosphate is the coenzyme form of Vitamin B1, and is a required intermediate in the pyruvate dehydrogenase complex and the ketoglutarate dehydrogenase complex. Thiamine pyrophosphate is necessary for oxidative phosphorylation and the pentose phosphate pathway by acting as a cofactor for α-ketoacid dehydrogenases[1][2][3].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vivo

    Thiamine pyrophosphate (25 mg/kg, i.p.) inhibits the ischemia-reperfusion induced oxidative damage in rat ovarian tissue[1].
    Thiamine pyrophosphate (20 mg/kg, i.p., once a day for 14 days) prevents Cisplatin (HY-17394) induced cardiotoxicity, and associated oxidative stress and heart injury in rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    460.77

    Formula

    C12H19ClN4O7P2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OP(OP(OCCC1=C(C)[N+](CC2=CN=C(C)N=C2N)=CS1)(O)=O)(O)=O.[Cl-]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 41.67 mg/mL (90.44 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1703 mL 10.8514 mL 21.7028 mL
    5 mM 0.4341 mL 2.1703 mL 4.3406 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (108.51 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (393 KB)

    COA (250 KB) HNMR (276 KB) CNMR (231 KB) RP-HPLC (205 KB) MS (228 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.1703 mL 10.8514 mL 21.7028 mL 54.2570 mL
    5 mM 0.4341 mL 2.1703 mL 4.3406 mL 10.8514 mL
    10 mM 0.2170 mL 1.0851 mL 2.1703 mL 5.4257 mL
    15 mM 0.1447 mL 0.7234 mL 1.4469 mL 3.6171 mL
    20 mM 0.1085 mL 0.5426 mL 1.0851 mL 2.7129 mL
    25 mM 0.0868 mL 0.4341 mL 0.8681 mL 2.1703 mL
    30 mM 0.0723 mL 0.3617 mL 0.7234 mL 1.8086 mL
    40 mM 0.0543 mL 0.2713 mL 0.5426 mL 1.3564 mL
    50 mM 0.0434 mL 0.2170 mL 0.4341 mL 1.0851 mL
    60 mM 0.0362 mL 0.1809 mL 0.3617 mL 0.9043 mL
    80 mM 0.0271 mL 0.1356 mL 0.2713 mL 0.6782 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Thiamine pyrophosphate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Thiamine pyrophosphate154-87-0CocarboxylaseEndogenous Metaboliteoxidative damageischemia-reperfusionInhibitorinhibitorinhibit

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