1. Metabolic Enzyme/Protease
  2. NEDD8-activating Enzyme
  3. ZM223

ZM223 

Cat. No.: HY-101790
Handling Instructions

ZM223 is a potent non-covalent NEDD8 activating enzyme (NAE) inhibitor, orally active.

For research use only. We do not sell to patients.

ZM223 Chemical Structure

ZM223 Chemical Structure

CAS No. : 2031177-48-5

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Description

ZM223 is a potent non-covalent NEDD8 activating enzyme (NAE) inhibitor, orally active[1].

IC50 & Target

NEDD8 activating enzyme (NAE)[1]

In Vitro

ZM223 (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC50s of 100 and 122 nM, respectively[1].
ZM223 (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex[1].

Cell Viability Assay[1]

Cell Line: HCT116 colon cancer cells and U-2OS osteosarcoma cells
Concentration: 0.1 μM, 1 μM
Incubation Time: 4 hours
Result: Inhibited both HCT-116 and U-2OS cancer cells.

Western Blot Analysis[1]

Cell Line: HCT116 colon cancer cells
Concentration: 0.1 μM, 1 μM
Incubation Time: 4 hours
Result: Caused a decrease in the level of NEDD8 and an increase in the downstream UBC12 protein.
Molecular Weight

502.53

Formula

C₂₃H₁₇F₃N₄O₂S₂

CAS No.

2031177-48-5

SMILES

O=C(C1=CC=C(C(F)(F)F)C=C1)NC2=NC3=CC=C(NC(CSC4=CC=C(N)C=C4)=O)C=C3S2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (165.82 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9899 mL 9.9497 mL 19.8993 mL
5 mM 0.3980 mL 1.9899 mL 3.9799 mL
10 mM 0.1990 mL 0.9950 mL 1.9899 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.14 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

ZM223ZM 223ZM-223NEDD8-activating EnzymeNAEInhibitorinhibitorinhibit

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ZM223
Cat. No.:
HY-101790
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