ABTL-0812
Based on 1 Customer Validation
ABTL-0812 (α-Hydroxylinoleic acid) induces endoplasmic reticulum (ER) stress-mediated autophagy. ABTL-0812 is a first-in-class small molecule with anti-cancer activity.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 57818-44-7
- Formula: C18H32O3
- Molecular Weight:296.44
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
ABTL-0812 (ABTL0812; 10-100 μM; 48 hours) inhibits cell viability of squamous NSCLC H157 cells[1].
Compared with squamous NSCLC H157 cells, human lung fibroblast cell line MRC-5 are resistant to ABTL0812 treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human lung fibroblast MRC5 and squamous non-small cell lung (NSCLC) H157 cells
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Concentration:10, 30, 100 μM
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Incubation Time:48 hours
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Result:Inhibited cell viability of squamous NSCLC H157 cells, not lung fibroblast cell line MRC-5.
ABTL-0812 induces hallmarks of ER stress in vivo. ABTL-0812 increases ATF4 and HSPA5 expression in mice bearing MiaPaca2 and A549 xenograft, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic female nude mice bearing MiaPaca2 and A549 xenograft models[1]
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Dosage:120 mg/kg
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Administration:Administered by oral gavage; 5 times per week for 33 days
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Result:Induced ER stress in human lung and pancreatic xenografts.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 57818-44-7
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Appearance Liquid (Density: 0.970±0.06 g/cm3)
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Molecular Weight 296.44
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Formula C18H32O3
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Color Colorless to off-white
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SMILES
CCCCC/C=C\C/C=C\CCCCCCC(O)C(O)=O
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Synonyms
α-Hydroxylinoleic acid
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 90 mg/mL (303.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3734 mL | 16.8668 mL | 33.7336 mL | 84.3341 mL |
| 5 mM | 0.6747 mL | 3.3734 mL | 6.7467 mL | 16.8668 mL | |
| 10 mM | 0.3373 mL | 1.6867 mL | 3.3734 mL | 8.4334 mL | |
| 15 mM | 0.2249 mL | 1.1245 mL | 2.2489 mL | 5.6223 mL | |
| 20 mM | 0.1687 mL | 0.8433 mL | 1.6867 mL | 4.2167 mL | |
| 25 mM | 0.1349 mL | 0.6747 mL | 1.3493 mL | 3.3734 mL | |
| 30 mM | 0.1124 mL | 0.5622 mL | 1.1245 mL | 2.8111 mL | |
| 40 mM | 0.0843 mL | 0.4217 mL | 0.8433 mL | 2.1084 mL | |
| 50 mM | 0.0675 mL | 0.3373 mL | 0.6747 mL | 1.6867 mL | |
| 60 mM | 0.0562 mL | 0.2811 mL | 0.5622 mL | 1.4056 mL | |
| 80 mM | 0.0422 mL | 0.2108 mL | 0.4217 mL | 1.0542 mL | |
| 100 mM | 0.0337 mL | 0.1687 mL | 0.3373 mL | 0.8433 mL |
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