AGN 192403 hydrochloride (Synonyms: (+)-BRD4780)

Name: AGN 192403 hydrochloride
Brand: MedChemExpress
SKU: HY-101374A
Rating: 4.5 (1 reviews)

Cat. No.: HY-101374A Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

AGN 192403 (BRD4780) hydrochloride is a potent and selective imidazoline-1 receptor antagonist with a K_i value of 42 nM. AGN 192403 hydrochloride is also a TMED9 inhibitor. AGN 192403 hydrochloride shows protective effects on oxidative cytotoxicity and mitochondrial inhibitor-induced cytotoxicity in astrocytes. AGN 192403 hydrochloride mitigates the proliferation and migration of differentiated glioma tumor cells. AGN 192403 hydrochloride can be used for glioma tumor and neurological diseases research.

For research use only. We do not sell to patients.

AGN 192403 hydrochloride Chemical Structure

CAS No. : 1021868-90-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of AGN 192403 hydrochloride:

(-)-AGN 192403 hydrochloride Get quote
AGN 192403 Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

imidazoline-1 receptor

42 nM (Ki)

Caspase-9

In Vitro

AGN 192403 (10-100 μM, 24 h) hydrochloride inhibits the former lysosomal destabilization and the subsequent decrease in mitochondrial potential in astrocytes exposed to Naphthazarin (HY-N7526)^[1].
AGN 192403 (10-100 μM, 24 h) hydrochloride inhibits the Antimycin A- and Rotenone (HY-B1756)-induced decrease in mitochondrial potential, cytochrome c release, caspase-9 activation, and eventually LDH release in astrocytes^[1].
AGN 192403 (4-25 μM, 14 days) hydrochloride exerts a remarkable inhibitory effect on glioma stem cells (GSC)s self-renewal, demonstrating almost complete inhibition of self-renewal at the highest concentration applied^[2].
AGN 192403 (0-25 μM, 18-48 h) hydrochloride targets TMED9, reduces the stemness, reduces GSC migration, and inhibits GSC tumorigenic functions^[2].
AGN 192403 (4-25 μM, 0-8 days) hydrochloride induces cell death in a dose dependent manner in patient-derived GSCs^[2].
AGN 192403 (4-25 μM, 48 h-8 days) hydrochloride inhibits TMED9 and exerts anti-tumor effects in diffuse intrinsic pontine glioma (DIPG) cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	patient-derived GSCs
Concentration:	0, 4, 10, 25 μM
Incubation Time:	48 h
Result:	Decreased TMED9 expression in a dose-dependent manner in three different patient-derived GSCs.

RT-PCR^[2]

Cell Line:	GSCs
Concentration:	10, 25 μM
Incubation Time:	48 h
Result:	Showed a marked and dose dependent reduction in SOX2 expression. Markedly reduced YKL40 expression.

Cell Migration Assay ^[2]

Cell Line:	GSCs
Concentration:	10, 25 μM
Incubation Time:	18-20 h
Result:	Dose-dependently reduced GSC migration.

Cell Viability Assay^[2]

Cell Line:	GSCs
Concentration:	4, 10, 25 μM
Incubation Time:	0, 1, 2, 3, 4, 5, 6, 7, 8 days
Result:	Resulted in a dose-dependent induction of cell death, at multiple points over the course of one week.

In Vivo

AGN 192403 (3000 μg/kg, i.v., single dose pretreat) hydrochloride can bloack imidazoline-1 receptor without modifying the baseline values of heart rate and blood pressure in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

189.73

Formula

C₁₀H₂₀ClN

CAS No.

1021868-90-5

Appearance

Solid

Color

White to off-white

SMILES

CC(C)[C@@H]1[C@@H](N)[C@H]2CC[C@@H]1C2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (527.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2706 mL	26.3532 mL	52.7065 mL
5 mM	1.0541 mL	5.2706 mL	10.5413 mL
10 mM	0.5271 mL	2.6353 mL	5.2706 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (281 KB) SDS (251 KB)

COA (253 KB) HNMR (241 KB) RP-HPLC (242 KB) LCMS (326 KB) Elemental Analysis Report (111 KB)

Handling Instructions (2659 KB)

References

[1]. Choi DH, et al. Protective effects of rilmenidine and AGN 192403 on oxidative cytotoxicity and mitochondrial inhibitor-induced cytotoxicity in astrocytes. Free Radic Biol Med. 2002 Nov 15;33(10):1321-33. [Content Brief]

[2]. Daoud Sarsour A, et al. Targeting the Cargo Receptor TMED9 as a Therapeutic Strategy Against Brain Tumors. Cells. 2025 May 23;14(11):772. [Content Brief]

[3]. Cobos-Puc LE, et al. α2A-adrenoceptors, but not nitric oxide, mediate the peripheral cardiac sympatho-inhibition of moxonidine. Eur J Pharmacol. 2016 Jul 5;782:35-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2706 mL	26.3532 mL	52.7065 mL	131.7662 mL
	5 mM	1.0541 mL	5.2706 mL	10.5413 mL	26.3532 mL
	10 mM	0.5271 mL	2.6353 mL	5.2706 mL	13.1766 mL
	15 mM	0.3514 mL	1.7569 mL	3.5138 mL	8.7844 mL
	20 mM	0.2635 mL	1.3177 mL	2.6353 mL	6.5883 mL
	25 mM	0.2108 mL	1.0541 mL	2.1083 mL	5.2706 mL
	30 mM	0.1757 mL	0.8784 mL	1.7569 mL	4.3922 mL
	40 mM	0.1318 mL	0.6588 mL	1.3177 mL	3.2942 mL
	50 mM	0.1054 mL	0.5271 mL	1.0541 mL	2.6353 mL
	60 mM	0.0878 mL	0.4392 mL	0.8784 mL	2.1961 mL
	80 mM	0.0659 mL	0.3294 mL	0.6588 mL	1.6471 mL
	100 mM	0.0527 mL	0.2635 mL	0.5271 mL	1.3177 mL

AGN 192403 hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AGN 1924031021868-90-5(+)-BRD4780AGN192403AGN-192403Imidazoline ReceptorCaspaseMitochondrial MetabolismLactate DehydrogenaseLDHcardiovascularHeart rateneurologicalAGN-192403 hydrochlorideAGN192403 hydrochlorideInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

AGN 192403 hydrochloride (Synonyms: (+)-BRD4780)

Customer Review

Other Forms of AGN 192403 hydrochloride:

AGN 192403 hydrochloride Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Caspase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

AGN 192403 hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AGN 192403 hydrochloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report