AI-10-104
Based on 1 Customer Validation
AI-10-104 is an inhibitor for runt-related transcription factor (RUNX), which enhances the cytotoxicity in myeloma cells.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.92%
- No. CAS: 1881276-00-1
- Fòrmula: C14H10F3N3O2
- Peso molecular:309.24
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
AI-10-104 (0-10 μM) inhibits the RUNX through dissociation of RUNXs-IKZFs complex, enhances the anti-proliferative effect of lenalidomide and its cytotoxicity in myeloma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T
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Concentration:0-10 μM
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Incubation Time:
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Result:Decreased levels of IKZF3 and CBFβ.
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Cell Line:OPM-1 cells, KMS-11 and U266 cells
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Concentration:1 μM
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Incubation Time:
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Result:Maintained the cell viability, inhibited proliferation of OPM-1 cells in a moderate effect.
Chemical Information
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No. CAS 1881276-00-1
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Appearance Solid
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Peso molecular 309.24
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Fòrmula C14H10F3N3O2
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Color Off-white to light yellow
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SMILES
FC(F)(F)OC1=CC=C2N=C(C3=NC=CC=C3OC)NC2=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 50 mg/mL (161.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2337 mL | 16.1687 mL | 32.3373 mL | 80.8434 mL |
| 5 mM | 0.6467 mL | 3.2337 mL | 6.4675 mL | 16.1687 mL | |
| 10 mM | 0.3234 mL | 1.6169 mL | 3.2337 mL | 8.0843 mL | |
| 15 mM | 0.2156 mL | 1.0779 mL | 2.1558 mL | 5.3896 mL | |
| 20 mM | 0.1617 mL | 0.8084 mL | 1.6169 mL | 4.0422 mL | |
| 25 mM | 0.1293 mL | 0.6467 mL | 1.2935 mL | 3.2337 mL | |
| 30 mM | 0.1078 mL | 0.5390 mL | 1.0779 mL | 2.6948 mL | |
| 40 mM | 0.0808 mL | 0.4042 mL | 0.8084 mL | 2.0211 mL | |
| 50 mM | 0.0647 mL | 0.3234 mL | 0.6467 mL | 1.6169 mL | |
| 60 mM | 0.0539 mL | 0.2695 mL | 0.5390 mL | 1.3474 mL | |
| 80 mM | 0.0404 mL | 0.2021 mL | 0.4042 mL | 1.0105 mL | |
| 100 mM | 0.0323 mL | 0.1617 mL | 0.3234 mL | 0.8084 mL |
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