Based on 1 Customer Validation
AM580 (CD336) (GMP) is AM580= (HY-10475) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. AM580 is a selective RARα agonist.
For research use only. We do not sell to patients.
- CAS No.: 102121-60-8
- Formula: C22H25NO3
- Molecular Weight:351.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-7 | EC50 |
0.3 nM
Compound: AM-580
|
Activity at human RARalpha ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
Activity at human RARalpha ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
|
[PMID: 19058965] |
| COS-7 | EC50 |
13 nM
Compound: AM-580
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Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
|
[PMID: 19058965] |
| COS-7 | EC50 |
8.6 nM
Compound: AM-580
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Activity at human RARbeta ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
Activity at human RARbeta ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay
|
[PMID: 19058965] |
| HL-60 | ED50 |
3.4 x 10-10M
Compound: 5
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Compound was tested for differentiation-inducing activity against human promyelocytic leukemia cell line HL-60
Compound was tested for differentiation-inducing activity against human promyelocytic leukemia cell line HL-60
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[PMID: 2795600] |
| Panel leukemia (Carcinoma cell lines) | ED50 |
3.4 x 10-10M
Compound: AM-580
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Effective dose for differentiation -inducing activity against human promyelocytic leukemia cells
Effective dose for differentiation -inducing activity against human promyelocytic leukemia cells
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[PMID: 3184125] |
AM580 (GMP) (0.05 μM) induces mouse embryonic fibroblasts (MEFs) convert into chemically induced pluripotent stem cells (CiPSCs) with the adding in stage I and stage II medium[1]. AM580 (GMP) (0-10 μM; 4 d) induces SH-SY5Y cells develop into neuron-like cells[2]. AM580 (GMP) shows powerful and selective cyto-differentiating effects on acute promyelocytic leukemia cells[3]. AM580 (GMP) (2.5 μM; 4 d) induces myeloid progenitors develop into neutrophils[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102121-60-8
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Appearance Solid
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Molecular Weight 351.44
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Formula C22H25NO3
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Color White to off-white
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SMILES
CC(CC1)(C)C2=C(C=CC(C(NC3=CC=C(C(O)=O)C=C3)=O)=C2)C1(C)C
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Synonyms
CD336 (GMP); NSC608001 (GMP); Ro 40-6055 (GMP)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhao T, et al. Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming. Cell Stem Cell. 2018 Jul 5;23(1):31-45.e7. [Content Brief]
[2]. Cai A, et al. Neuroblastoma SH-SY5Y Cell Differentiation to Mature Neuron by AM580 Treatment. Neurochem Res. 2022 [Content Brief]
[3]. Gianní M, et al. AM580, a stable benzoic derivative of retinoic acid, has powerful and selective cyto-differentiating effects on acute promyelocytic leukemia cells. Blood. 1996 Feb 15;87(4):1520-31. [Content Brief]
[4]. Brok-Volchanskaya VS, et al. Effective and Rapid Generation of Functional Neutrophils from Induced Pluripotent Stem Cells Using ETV2-Modified mRNA. Stem Cell Reports. 2019 Dec 10;13(6):1099-1110. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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