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Cat. No.: HY-W319584

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AMP-404

AMP-404 is an Imexon (HY-15385)-derived antitumor agent with dual functions as a cytotoxin and growth inhibitor. AMP-404 exerts significant cytotoxic effects against various sensitive, drug-resistant and primary human tumor cells, including myeloma, breast cancer, melanoma, ovarian cancer and sarcoma cells. AMP-404 effectively inhibits the growth of rat PIE 2-3 sarcoma in vivo and exhibits remarkable in vivo efficacy.

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CAS No.: 55275-64-4
Formule: C₁₀H₉N₃O
Masse moléculaire:187.20

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

AMP-404 Chemical Structure

CAS No.: 55275-64-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Protocole (2)
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	11 μM Compound: 13	Inhibition of cell growth in (A375) melan cell line Inhibition of cell growth in (A375) melan cell line	[PMID: 9986721]
A549	IC₅₀	9 μM Compound: 13	Inhibition of cell growth in (A-549) lung cell line Inhibition of cell growth in (A-549) lung cell line	[PMID: 9986721]
L1210	IC₅₀	1.2 μM Compound: 13	Inhibition of cell growth in drug sensitive L1210 leukemia cell line determined by MTT assay Inhibition of cell growth in drug sensitive L1210 leukemia cell line determined by MTT assay	[PMID: 9986721]
L1210	IC₅₀	11 μM Compound: 13	Inhibition of cell growth in (L1210 leukemia) MDR cells determined by MTT assay Inhibition of cell growth in (L1210 leukemia) MDR cells determined by MTT assay	[PMID: 9986721]
MCF7	IC₅₀	2.6 μM Compound: 13	Inhibition of cell growth in (MCF7 breast) dox cell line Inhibition of cell growth in (MCF7 breast) dox cell line	[PMID: 9986721]
MCF7	IC₅₀	2.9 μM Compound: 13	Inhibition of cell growth in drug sensitive MCF-7 breast cell line Inhibition of cell growth in drug sensitive MCF-7 breast cell line	[PMID: 9986721]
MCF7	IC₅₀	4.3 μM Compound: 13	Inhibition of cell growth in (MCF7 breast) mitox cell line Inhibition of cell growth in (MCF7 breast) mitox cell line	[PMID: 9986721]
OVCAR-3	IC₅₀	6.8 μM Compound: 13	Inhibition of cell growth in (OVCAR-3) ovarian cell line Inhibition of cell growth in (OVCAR-3) ovarian cell line	[PMID: 9986721]
RPMI-8226	IC₅₀	5.3 μM Compound: 6	Cytotoxicity against multidrug resistant (MDR) human myeloma 8226 cells by MTT assay Cytotoxicity against multidrug resistant (MDR) human myeloma 8226 cells by MTT assay	[PMID: 14695835]
RPMI-8226	IC₅₀	5.3 μM Compound: 13	Inhibition of cell growth in drug sensitive 8226 myeloma cell line Inhibition of cell growth in drug sensitive 8226 myeloma cell line	[PMID: 9986721]
RPMI-8226	IC₅₀	8 μM Compound: 13	Inhibition of cell growth in drug resistant 8226 myeloma cell line Inhibition of cell growth in drug resistant 8226 myeloma cell line	[PMID: 9986721]
WiDr	IC₅₀	12 μM Compound: 13	Inhibition of cell growth in drug resistant WiDr colon cell line Inhibition of cell growth in drug resistant WiDr colon cell line	[PMID: 9986721]
WiDr	IC₅₀	5 μM Compound: 13	Inhibition of cell growth in drug sensitive WiDr colon cell line Inhibition of cell growth in drug sensitive WiDr colon cell line	[PMID: 9986721]

In Vitro

AMP-404 (compound 13) potently inhibits the growth of a variety of established human and murine tumor cell lines, including drug-resistant mutant strains, with an IC₅₀ value range of 1.2 μM (murine sensitive L1210 leukemia) to 12 μM (human multidrug-resistant WiDr colon carcinoma)^[1].
AMP-404 (0.2-2.0 μM; 24 h) inhibits colony growth of various freshly isolated human tumor cells, with the strongest activity against myeloma, gastric cancer and sarcoma^[1].
The log (1/C) value of the anti-myeloma efficacy of AMP-404 is 5.27, which is consistent with the positive correlation between lipophilicity and cytotoxicity in human-sensitive multiple myeloma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

AMP-404 (compound 13) (10-fold dose range) exhibits antitumor activity against PIE 2-3 sarcoma in Wistar rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 55275-64-4
Masse moléculaire 187.20
Formule C₁₀H₉N₃O
SMILES

N#CC1N(C(=O)NC=2C=CC=CC2)C1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Animals and Diseases Cell Biology

Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Dvorakova K. Mechanisms of imexon action in human myeloma cells[M]. The University of Arizona, 2000.

[2]. Iyengar BS, et al. Novel antitumor 2-cyanoaziridine-1-carboxamides. J Med Chem. 1999;42(3):510-514. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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