Angstrom6 (Synonyms: A6 Peptide)

Name: Angstrom6
Brand: MedChemExpress
SKU: HY-P2230
Rating: 4.5 (1 reviews)

Cat. No.: HY-P2230 Purity: 99.01%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Angstrom6 (A6 Peptide) is an 8 amino-acid peptide derived from single-chain urokinase plasminogen activator (scuPA) and interferes with the uPA/uPAR cascade and abrogates downstream effects. Angstrom6 binds to CD44 resulting in the inhibition of migration, invasion, and metastasis of tumor cells, and the modulation of CD44-mediated cell signaling.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Angstrom6 Chemical Structure

CAS No. : 220334-14-5

Size	Price	Stock	Quantity
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

Angstrom6 is effective at blocking the migration of the OVCAR8, OVCAR3, ES2, IGROV-1, MDA-MB-468, and MDA-MB361 cells with IC₅₀s in the range of 10 to 100 nM^[3].
Angstrom6 potentiates the CD44-dependent adhesion of cancer cells to hyaluronic acid and activated CD44-mediated signaling, as evidenced by focal adhesion kinase and MAP/ERK kinase phosphorylation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Angstrom6 (100 mg/kg; s.c. twice daily) reduces the number of lung foci generated by the i.v. injection of B16-F10 melanoma cells by 50%^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6 mice (bearing B16-F10 cells)^[3]
Dosage:	100 mg/kg
Administration:	S.c.; twice a day for 11 days
Result:	Reduced the number of lung nodules and reduced the number of lung metastases to 50% of control.

Molecular Weight

910.97

Formula

C₃₉H₆₂N₁₀O₁₅

CAS No.

220334-14-5

Appearance

Solid

Color

White to off-white

Sequence

Ac-Lys-Pro-Ser-Ser-Pro-Pro-Glu-Glu-NH2

Sequence Shortening

Ac-KPSSPPEE-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (109.77 mM)

DMSO : 100 mg/mL (109.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0977 mL	5.4887 mL	10.9773 mL
5 mM	0.2195 mL	1.0977 mL	2.1955 mL
10 mM	0.1098 mL	0.5489 mL	1.0977 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.01%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB) RP-HPLC (203 KB) Elemental Analysis Report (110 KB)

Handling Instructions (2659 KB)

References

[1]. Ghamande SA, et al. A phase 2, randomized, double-blind, placebo-controlled trial of clinical activity and safety of subcutaneous A6 in women with asymptomatic CA125 progression after first-line chemotherapy of epithelial ovarian cancer. Gynecol Oncol. 2008;111(1):89-94. [Content Brief]

[2]. Finlayson M. Modulation of CD44 Activity by A6-Peptide. Front Immunol. 2015;6:135. Published 2015 Mar 30. [Content Brief]

[3]. Piotrowicz RS, et al. A6 peptide activates CD44 adhesive activity, induces FAK and MEK phosphorylation, and inhibits the migration and metastasis of CD44-expressing cells. Mol Cancer Ther. 2011;10(11):2072-2082. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.0977 mL	5.4887 mL	10.9773 mL	27.4433 mL
	5 mM	0.2195 mL	1.0977 mL	2.1955 mL	5.4887 mL
	10 mM	0.1098 mL	0.5489 mL	1.0977 mL	2.7443 mL
	15 mM	0.0732 mL	0.3659 mL	0.7318 mL	1.8296 mL
	20 mM	0.0549 mL	0.2744 mL	0.5489 mL	1.3722 mL
	25 mM	0.0439 mL	0.2195 mL	0.4391 mL	1.0977 mL
	30 mM	0.0366 mL	0.1830 mL	0.3659 mL	0.9148 mL
	40 mM	0.0274 mL	0.1372 mL	0.2744 mL	0.6861 mL
	50 mM	0.0220 mL	0.1098 mL	0.2195 mL	0.5489 mL
	60 mM	0.0183 mL	0.0915 mL	0.1830 mL	0.4574 mL
	80 mM	0.0137 mL	0.0686 mL	0.1372 mL	0.3430 mL
	100 mM	0.0110 mL	0.0549 mL	0.1098 mL	0.2744 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Angstrom6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Angstrom6220334-14-5A6 PeptideAngstrom 6Angstrom-6PAI-1Plasminogen activator inhibitor-1single-chainurokinaseplasminogenactivatorCD44migrationinvasionmetastasiscancerInhibitorinhibitorinhibit

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Angstrom6 (Synonyms: A6 Peptide)

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Angstrom6 Related Antibodies

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Angstrom6 Related Antibodies

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Complete Stock Solution Preparation Table

Angstrom6 Related Classifications

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