Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7)
Based on 1 Customer Validation
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) is an anti-mouse CD106/VCAM-1 IgG1 monoclonal antibody. Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) reduces inflammatory response and oxidative stress by lowering p-STAT3 and reactive oxygen species (ROS) levels. Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) can alleviate cardiac inflammation and fibrosis by reducing the expression of collagen I and collagen III. Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) can be used for research on cardiovascular conditions such as hypertensive heart condition and subretinal fibrosis.
For research use only. We do not sell to patients.
- Purity : 95.00%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
[3]|
IL-1β |
IL-6 |
p-STAT3 |
Collagen I |
Collagen III |
In Vitro
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) (25 μg/mL, 4 h) can significantly inhibit VCAM-1 mediated monocyte adhesion with an inhibition rate of 52 % in TNF-α activated murine cardiac endothelial cells (MCEC) cells[1].
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) (20 μg/mL, 10 h) can block bone marrow-derived macrophages (BMDMs) migration[2].
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) (5 μg/mL, 10 h) significantly inhibits Ang II induced BMDMs adhesion and migration[3].
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) (5 μg/mL, 24 h) inhibits myocardial cell hypertrophy and fibroblast activation by reducing macrophage derived inflammatory factors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BMDMs
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Concentration:20 μg/mL
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Incubation Time:10 h
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Result:Significantly reduced the number of macrophage migration caused by VCAM-1.
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Cell Line:BMDMs
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Concentration:5 μg/mL
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Incubation Time:10 h
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Result:Significantly reduced the number of cell migrations caused by Ang II.
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Cell Line:Rat cardiomyocytes and cardiac fibroblasts
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Concentration:5 μg/mL
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Incubation Time:24 h
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Result:Reduced the level of CaNA, p-STAT3, TGF-β1 and p-Smad2/3.
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Cell Line:Rat cardiomyocytes and cardiac fibroblasts
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Concentration:5 μg/mL
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Incubation Time:24 h
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Result:Reduced the level of ANF, BNP, collagen I and collagen III.
In Vivo
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) (0.1 mg or 0.2 mg, i.p., once every 2 days, for 14 days) dose dependently improves cardiac dysfunction and reduces cardiac inflammation and fibrosis in wild-type male C57BL/6J mice with a Ang II induced hypertensive cardiac remodeling model[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Laser induced C57BL/6J mice[2]
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Dosage:6.85 μg/eye
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Administration:Intravitreal injection, single dose immediately after the second laser
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Result:Significantly reduced the area of collagen-1 positive fibrosis.
Reduced F4/80+ macrophage infiltration.
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Animal Model:Ang II (1,000 ng/kg/min, s.c., for 14 days) implanted wild-type male C57BL/6J mice[3]
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Dosage:0.1 mg or 0.2 mg
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Administration:Intraperitoneal injection (i.p.), once every 2 days, for 14 days
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Result:Improved the heart function decline caused by Ang II.
Reduced heart weight/body weight ratio and ANF/BNP/MYH7 mRNA expression.
Inhibited collagen deposition, collagen III, and α-SMA expression.
Reduced CD68+/VLA-4+ macrophage infiltration, IL-1β/IL-6/TNF-α mRNA expression, and ROS generation.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo VCAM-1 neutralization; Immunofluorescence
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Kelly KA, et al. Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle. Circ Res. 2005 Feb 18;96(3):327-36. [Content Brief]
[2]. Deng W, et al. Vascular Cell Adhesion Molecule-1 (VCAM-1) contributes to macular fibrosis in neovascular age-related macular degeneration through modulating macrophage functions. Immun Ageing. 2023 Nov 20;20(1):65. [Content Brief]
[3]. Qiu ZY, et al. Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration. Front Pharmacol. 2022 Nov 17;13:1058268. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD106/VCAM-1 Antibody (M/K-2.7) Related Classifications
HY-P990294 Related Classifications
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