8-BuS-AMP
Based on 1 Customer Validation
8-BuS-AMP is a NTPDase1 inhibitor and a CD73/CD39 inhibitor, with an IC50 of 35 μM and a Ki value of 0.292 μM against human NTPDase1; its Ki values against human CD73 and CD39 are 1.19 μM and 0.847 μM, respectively. 8-BuS-AMP binds to the substrate-binding pockets of NTPDase1 and CD73 to effectively block the conversion of ATP and AMP to adenosine, thereby enhancing the activation and proliferation of human peripheral T lymphocytes. 8-BuS-AMP possesses excellent enzymatic hydrolysis resistance and metabolic stability, resists hydrolysis by multiple NTPDase subtypes, and shows no activity against P2Y1 and P2Y12 receptors. 8-BuS-AMP can be used in purinergic signaling pathway and cancer-related studies.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.90%
- CAS. Nr.: 344402-39-7
- Formel: C14H22N5O7PS
- Molecular Weight:435.39
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
8-BuS-AMP (100 μM; 10 min) impairs recombinant human NTPDase1's ability to inhibit ADP-induced platelet aggregation, but does not itself induce or inhibit platelet aggregation, indicating in vitro inactivity towards platelet P2Y1 and P2Y12 receptors[2].
8-BuS-AMP (1 μM; 0, 20, 40, 60 min) is highly metabolically stable in human and mouse liver microsomes, with half-lives of 462 min and 182 min, respectively[3].
8-BuS-AMP (1-100 μM; 1 h) concentration-dependently inhibits nucleotide hydrolysis in human synovial fluid, reducing adenosine formation from both ε-ATP and ε-AMP[3].
8-BuS-AMP (10-100 μM; 3 days, added daily) concentration-dependently enhances activation (via increased CD25 expression) and proliferation of human primary CD4+ and CD8+ T lymphocytes without reducing cell viability[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 344402-39-7
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Appearance Solid
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Molecular Weight 435.39
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Formel C14H22N5O7PS
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2SCCCC)O[C@@H]1COP(O)(O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
H2O : 50 mg/mL (114.84 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Baqi Y, et al. Development of Anthraquinone Derivatives as Ectonucleoside Triphosphate Diphosphohydrolase (NTPDase) Inhibitors With Selectivity for NTPDase2 and NTPDase3. Front Pharmacol. 2020;11:1282. Published 2020 Aug 27. [Content Brief]
[2]. Lecka J, et al. 8-BuS-ATP derivatives as specific NTPDase1 inhibitors. Br J Pharmacol. 2013;169(1):179-196. [Content Brief]
[3]. Bi C, et al. Synthesis, Characterization, Interactions, and Immunomodulatory Function of Ectonucleotidase CD39/CD73 Inhibitor 8-Butylthioadenosine 5'-Monophosphate. ACS Pharmacol Transl Sci. 2025;8(5):1401-1415. Published 2025 Apr 3. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.2968 mL | 11.4840 mL | 22.9679 mL | 57.4198 mL |
| 5 mM | 0.4594 mL | 2.2968 mL | 4.5936 mL | 11.4840 mL | |
| 10 mM | 0.2297 mL | 1.1484 mL | 2.2968 mL | 5.7420 mL | |
| 15 mM | 0.1531 mL | 0.7656 mL | 1.5312 mL | 3.8280 mL | |
| 20 mM | 0.1148 mL | 0.5742 mL | 1.1484 mL | 2.8710 mL | |
| 25 mM | 0.0919 mL | 0.4594 mL | 0.9187 mL | 2.2968 mL | |
| 30 mM | 0.0766 mL | 0.3828 mL | 0.7656 mL | 1.9140 mL | |
| 40 mM | 0.0574 mL | 0.2871 mL | 0.5742 mL | 1.4355 mL | |
| 50 mM | 0.0459 mL | 0.2297 mL | 0.4594 mL | 1.1484 mL | |
| 60 mM | 0.0383 mL | 0.1914 mL | 0.3828 mL | 0.9570 mL | |
| 80 mM | 0.0287 mL | 0.1435 mL | 0.2871 mL | 0.7177 mL | |
| 100 mM | 0.0230 mL | 0.1148 mL | 0.2297 mL | 0.5742 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
8-BuS-AMP Related Classifications
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