Art. -Nr.: HY-12336

NIBR189

Name: NIBR189
Brand: MedChemExpress
SKU: HY-12336
Price: 100.00 USD
Availability: InStock
Rating: 5 (3 reviews)

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

NIBR189 is an EBI2 (Epstein-Barr virus-induced gene 2) antagonist. NIBR189 inhibits human and mouse EBI2 with IC₅₀s of 11 and 16 nM, respectively. NIBR189 can be used for the research of autoimmune diseases.

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Reinheit : 99.71%
CAS. Nr.: 1599432-08-2
Formel: C₂₁H₂₁BrN₂O₃
Molecular Weight:429.31

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

NIBR189 Chemical Structure

CAS. Nr.: 1599432-08-2

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	11 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]
CHO	IC₅₀	15 nM Compound: 4m, NIBR189	Antagonist activity at recombinant mouse EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay Antagonist activity at recombinant mouse EBI2 receptor expressed in CHO cells assessed as inhibition of NIBR51-induced intracellular calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]
CHO	IC₅₀	7 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.1 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.1 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis	[PMID: 24678947]
CHO	IC₅₀	8.5 nM Compound: 4m, NIBR189	Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.33 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis Antagonist activity at recombinant human EBI2 receptor expressed in CHO cells assessed as inhibition of 0.33 nM oxysterol-induced [35S]GTPgammaS binding after 1 hr by scintillation counting analysis	[PMID: 24678947]
U-937	IC₅₀	0.3 nM Compound: 4m, NIBR189	Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of 7-alpha,25-OHC-induced cell migration after 3 hrs by flow cytometric analysis Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of 7-alpha,25-OHC-induced cell migration after 3 hrs by flow cytometric analysis	[PMID: 24678947]
U-937	IC₅₀	4.5 nM Compound: 4; NIBR189	Antimigratory activity against human U-937 cells assessed as inhibition of 7alpha,25-dihydroxycholesterol induced cell migration by CCK-8 method Antimigratory activity against human U-937 cells assessed as inhibition of 7alpha,25-dihydroxycholesterol induced cell migration by CCK-8 method	[PMID: 38417036]
U-937	IC₅₀	9 nM Compound: 4m, NIBR189	Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of oxysterol-induced calcium release measured for 3 mins by FLIPR assay Antagonist activity at EBI2 receptor in human U937 cells assessed as inhibition of oxysterol-induced calcium release measured for 3 mins by FLIPR assay	[PMID: 24678947]

In Vitro

NIBR189 (0-1 μM; 3 h) blocks migration of U937 cells^[1].
NIBR189 (0-10 μM) blocks oxysterol-dependent activation with an IC₅₀ value of 9 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay ^[1]

Cell Line:

U937 cell lines
Concentration:

0-1 μM
Incubation Time:

3 hours
Result:

Blocked the direct migration of U937 cells with an IC₅₀ value of 0.3 nM.

In Vivo

1.19 Pharmacokinetic Properties of NIBR189 in Mice^[1].

	Mice IV 1 mg/kg	Mice PO 3 mg/kg
CL (μL/min/mg)	16
t_1/2 (h)	1.1
V_ss (L/kg)	1.4
AUC (nmol·h/L)	2435	3608
C_max (nmol/L)		835
t_max (h)		1
F (%)		49

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 1599432-08-2
Appearance Solid
Molecular Weight 429.31
Formel C₂₁H₂₁BrN₂O₃
Color White to off-white
SMILES

O=C(N1CCN(C(C2=CC=C(OC)C=C2)=O)CC1)/C=C/C3=CC=C(Br)C=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

All Cell Proliferation/Viability AssayFlow CytometryMicrobiological Assay

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Journal Impact Factor

Nat Aging

Mitochondria-enriched hematopoietic stem cells exhibit elevated self-renewal capabilities, thriving within the context of aged bone marrow. [Abstract]

2025 May;5(5):831-847. PMID: 40050412

NIBR189 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 May;5(5):831-847. [Abstract]

Relative mito-Dendra2 expression following NIBR189 (25 μg/mL; 48 h) and/or 7α,25-DHC treatment, with normalized MFI values (n = 3).
Nat Commun

Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]

2026 Feb 12;17(1):1214. PMID: 41680175
Microb Pathog

Downregulation of GPR183 on infection restricts the early infection and intracellular replication of mycobacterium tuberculosis in macrophage. [Abstract]

2020 Aug:145:104234. PMID: 32353576

NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug:145:104234. [Abstract]

The cell viability under the treatment of NIBR189 (6.4-25 μM; 24-72 h) and 7α,25-OHC at different time points changes little, determined by MTS assay (n = 5).

NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug:145:104234. [Abstract]

Bacterial early infection (4 h poi) is compromised under the treatment of NIBR189 (25 nM) and 7α,25-OHC in RAW264.7. (CFU in left panel, TTD in right panel).

NIBR189 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2020 Aug:145:104234. [Abstract]

The effect of NIBR189 (25 nM) and 7α,25-OHC on intracellular Mtb replication is compared using cfu at different time points after infection in RAW264.7 (n = 5). NIBR189 reduced the intracellular Mtb at the stages after phagocytosis.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (116.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3293 mL

11.6466 mL

23.2932 mL

5 mM

0.4659 mL

2.3293 mL

4.6586 mL

10 mM

0.2329 mL

1.1647 mL

2.3293 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (5.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Gessier F, et al. Identification and characterization of small molecule modulators of the Epstein-Barr virus-induced gene 2 (EBI2) receptor. J Med Chem. 2014 Apr 24;57(8):3358-68. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3293 mL	11.6466 mL	23.2932 mL	58.2330 mL
	5 mM	0.4659 mL	2.3293 mL	4.6586 mL	11.6466 mL
	10 mM	0.2329 mL	1.1647 mL	2.3293 mL	5.8233 mL
	15 mM	0.1553 mL	0.7764 mL	1.5529 mL	3.8822 mL
	20 mM	0.1165 mL	0.5823 mL	1.1647 mL	2.9116 mL
	25 mM	0.0932 mL	0.4659 mL	0.9317 mL	2.3293 mL
	30 mM	0.0776 mL	0.3882 mL	0.7764 mL	1.9411 mL
	40 mM	0.0582 mL	0.2912 mL	0.5823 mL	1.4558 mL
	50 mM	0.0466 mL	0.2329 mL	0.4659 mL	1.1647 mL
	60 mM	0.0388 mL	0.1941 mL	0.3882 mL	0.9705 mL
	80 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7279 mL
	100 mM	0.0233 mL	0.1165 mL	0.2329 mL	0.5823 mL

Bioz

Keywords

NIBR189

3 Cited Publications

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NIBR189

Publications Citing Use of MedChemExpress (MCE) NIBR189