RN-18
Based on 1 Customer Validation
RN-18 is a HIV-1 viral infectivity factor (HIV-1 Vif) inhibitor with an IC50 of 6 μM in nonpermissive H9 cells.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.75%
- CAS. Nr.: 431980-38-0
- Formel: C20H16N2O4S
- Molecular Weight:380.42
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
IC50: 6 μM (nonpermissive H9 cell)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
4.5 μM
Compound: 1, RN-18
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Inhibition of X4-tropic HIV-1 LAI Vif infected in CEM cells assessed as inhibition of viral replication incubated for 14 to 21 days measured on every other day by reverse transcriptase assay
Inhibition of X4-tropic HIV-1 LAI Vif infected in CEM cells assessed as inhibition of viral replication incubated for 14 to 21 days measured on every other day by reverse transcriptase assay
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[PMID: 24533175] |
| H9 | IC50 |
6 μM
Compound: 1, RN-18
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Inhibition of X4 tropic HIV-1 LAI Vif infected in H9 cells assessed as inhibition of viral replication incubated for 10 to 15 days measured on every second day by reverse transcriptase assay
Inhibition of X4 tropic HIV-1 LAI Vif infected in H9 cells assessed as inhibition of viral replication incubated for 10 to 15 days measured on every second day by reverse transcriptase assay
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[PMID: 24533175] |
| H9 | IC50 |
6 μM
Compound: 1a; RN-18
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Antagonist activity against HIV-1 Vif in human H9 cells expressing A3G assessed as inhibition of viral infection measured after 16 hrs by cell-based assay
Antagonist activity against HIV-1 Vif in human H9 cells expressing A3G assessed as inhibition of viral infection measured after 16 hrs by cell-based assay
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[PMID: 27509004] |
| HEK293 | EC50 |
5.8 μM
Compound: RN-18
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Inhibition of HIV Vif induced human cytidine-deaminase degradation in double transgenic HEK293 cells constitutively expressing enhanced yellow fluorescent protein expressor (EYFP-A3G) fusion and Tet-Off controllable Vif protein after 24 hrs by fluorimetri
Inhibition of HIV Vif induced human cytidine-deaminase degradation in double transgenic HEK293 cells constitutively expressing enhanced yellow fluorescent protein expressor (EYFP-A3G) fusion and Tet-Off controllable Vif protein after 24 hrs by fluorimetri
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[PMID: 19841153] |
| MT4 | IC50 |
>100 μM
Compound: 1, RN-18
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Inhibition of X4-tropic HIV-1 LAI Vif infected in MT4 cells assessed as inhibition of viral replication incubated for 14 to 21 days measured on every other day by reverse transcriptase assay
Inhibition of X4-tropic HIV-1 LAI Vif infected in MT4 cells assessed as inhibition of viral replication incubated for 14 to 21 days measured on every other day by reverse transcriptase assay
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[PMID: 24533175] |
RN-18 and RN-19 exhibits potent antiviral activity in the nonpermissive H9 and CEM cells but not in MT4 or CEM-SS cells, confirming that the antiviral activity was Vif specific. RN-18 shows the greater potency (IC50=4.5 μM in CEM cells) and specificity (IC50>100 μM in MT4 cells) among the two compounds[1]. In the presence of the inhibitor, RN-18, reverse transcriptase activity in the nonpermissive H9 and CEM cells decreases substantially and in a dose-dependent manner. RN-18 also exhibits antiviral activity in CEM-SS modified to stably express A3G but does not exhibit antiviral activity in the parental CEM-SS cell line. RN-18 antagonizes Vif function and inhibits HIV-1 replication only in the presence of A3G. RN-18 increases cellular A3G levels in a Vif-dependent manner and increases A3G incorporation into virions without inhibiting general proteasome-mediated protein degradation. RN-18 enhances Vif degradation only in the presence of A3G, reduces viral infectivity by increasing A3G incorporation into virions and enhances cytidine deamination of the viral genome[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 431980-38-0
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Appearance Solid
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Molecular Weight 380.42
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Formel C20H16N2O4S
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Color Light yellow to yellow
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SMILES
O=C(NC1=CC=CC=C1OC)C2=CC=CC=C2SC3=CC=C([N+]([O-])=O)C=C3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (262.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
H9 or MT4 cells are treated overnight with 0, 1, 5, 10, 25 or 50 μM RN-18 (all at 0.1% DMSO) and infected with HIV-1. All cells are maintained in the presence of DMSO or RN-18 for 14 d, and viral replication is monitored every 2 d by measuring reverse transcriptase activity in culture supernatants. The average % relative infectivity at day 7 is determined from 3 separate reverse transcriptase assays. Grafit software is used to fit curves and to determine IC50[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6287 mL | 13.1434 mL | 26.2867 mL | 65.7168 mL |
| 5 mM | 0.5257 mL | 2.6287 mL | 5.2573 mL | 13.1434 mL | |
| 10 mM | 0.2629 mL | 1.3143 mL | 2.6287 mL | 6.5717 mL | |
| 15 mM | 0.1752 mL | 0.8762 mL | 1.7524 mL | 4.3811 mL | |
| 20 mM | 0.1314 mL | 0.6572 mL | 1.3143 mL | 3.2858 mL | |
| 25 mM | 0.1051 mL | 0.5257 mL | 1.0515 mL | 2.6287 mL | |
| 30 mM | 0.0876 mL | 0.4381 mL | 0.8762 mL | 2.1906 mL | |
| 40 mM | 0.0657 mL | 0.3286 mL | 0.6572 mL | 1.6429 mL | |
| 50 mM | 0.0526 mL | 0.2629 mL | 0.5257 mL | 1.3143 mL | |
| 60 mM | 0.0438 mL | 0.2191 mL | 0.4381 mL | 1.0953 mL | |
| 80 mM | 0.0329 mL | 0.1643 mL | 0.3286 mL | 0.8215 mL | |
| 100 mM | 0.0263 mL | 0.1314 mL | 0.2629 mL | 0.6572 mL |
RN-18 Related Classifications
HY-102014 Related Classifications
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