Art. -Nr.: HY-107433

U18666A

29 Cited Publications

Kundenbewertung

Based on 29 publication(s) in Google Scholar

U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.92%
CAS. Nr.: 3039-71-2
Formel: C₂₅H₄₂ClNO₂
Molecular Weight:424.06

Speicherung:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

U18666A Chemical Structure

CAS. Nr.: 3039-71-2

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (29)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus^[1].

IC₅₀ & Target

Cholesterol^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	>50 μM Compound: U18666A	Cytotoxicity in human HL60 cells by MTT assay Cytotoxicity in human HL60 cells by MTT assay	[PMID: 28964935]
Huh-7	CC₅₀	34.4 μM Compound: 67	Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay	[PMID: 26771861]

In Vitro

U18666A, the antiviral effect is found to result from two events: retarded viral trafficking in the cholesterol-loaded late endosomes/lysosomes and suppresse de novo sterol biosynthesis in treated infected cells. It is also observed an additive antiviral effect of U18666A with C75, a fatty acid synthase inhibitor, suggesting dengue virus relies on both the host cholesterol and fatty acid biosynthesis for successful replication^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 3039-71-2
Appearance Solid
Molecular Weight 424.06
Formel C₂₅H₄₂ClNO₂
Color White to off-white
SMILES

C[C@@]12[C@]3([H])[C@](CC=C1C[C@@H](OCCN(CC)CC)CC2)([H])[C@@]4([H])[C@@](C)(C(CC4)=O)CC3.Cl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (29)

All ELISABio/Physico-chemical AssayCell Imaging/StainingWBIF

Sort by:

Journal Impact Factor

Cell

Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic. [Abstract]

2026 Feb 5;189(3):818-831.e19. PMID: 41576953
Nat Microbiol

Lipid accumulation in tuberculosis granulomas inhibits macrophage-CD4+ T cell interactions and infection control. [Abstract]

2026 Jun;11(6):1677-1695. PMID: 41933199
Nat Commun

Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]

2026 Feb 12;17(1):1214. PMID: 41680175
Nat Commun

Cholesterol-rich lysosomes induced by respiratory syncytial virus promote viral replication by blocking autophagy flux. [Abstract]

2024 Jul 26;15(1):6311. PMID: 39060258

U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]

Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated HEp-2 cells.

U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]

Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated 16HBE cells.
Mol Cell

mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport. [Abstract]

2025 Sep 18;85(18):3486-3504.e7. PMID: 40972529
J Control Release

Reversing immunotherapy resistance in cold tumors by weaponizing pyroptosis with a dual-payload nanotuner. [Abstract]

2026 Jun 18:396:115116. PMID: 42314991
Oncogene

The NPC1/USP7/p53 axis regulates cholesterol and promotes the proliferation of hepatocellular carcinoma. [Abstract]

2026 Apr;45(15):1386-1397. PMID: 41882104
Cell Chem Biol

FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase. [Abstract]

2024 Mar 8:S2451-9456(24)00081-3. PMID: 38513646
Transl Psychiatry

Fluoxetine disrupts cholesterol metabolism in endothelial cells via SREBP2 activation. [Abstract]

2026 Jun 23;16(1):318. PMID: 42337263
Br J Pharmacol

Curcumin controls lysosomal acidification and exocytosis to ameliorate lysosomal cholesterol accumulation in Niemann-Pick type C disease. [Abstract]

2026 Apr 26. PMID: 42036241
Emerg Microbes Infect

Hepatitis B virus promotes its own replication by enhancing RAB5A-mediated dual activation of endosomal and autophagic vesicle pathways. [Abstract]

2023 Dec;12(2):2261556. PMID: 37725090

U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]

HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours. The levels of secreted HBsAg in the culture supernatant and intracellular HBsAg in the cell lysate were detected by ELISA.

U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]

HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours, and HBcAg was detected by WB.
Cell Mol Life Sci

Human cytomegalovirus infection impairs neural differentiation via repressing sterol regulatory element binding protein 2-mediated cholesterol biosynthesis. [Abstract]

2024 Jul 6;81(1):289. PMID: 38970696

U18666A purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Jul 6;81(1):289. [Abstract]

Intracellular cholesterol contents measured in SHEDs with different treatment. Cells added U18666A (2 µg/mL), an inhibitor specific to cholesterol synthesis, were measured as positive reference.
Neurobiol Dis

LRRK2 and RAB8A regulate cell death after lysosomal damage in macrophages through cholesterol-related pathways. [Abstract]

2024 Nov:202:106728. PMID: 39521098
Int J Mol Sci

Hepatocyte-Derived Prostaglandin E2-Modulated Macrophage M1-Type Polarization via mTOR-NPC1 Axis-Regulated Cholesterol Transport from Lysosomes to the Endoplasmic Reticulum in Hepatitis B Virus x Protein-Related Nonalcoholic Steatohepatitis. [Abstract]

2022 Oct 1;23(19):11660. PMID: 36232960
J Dairy Sci

Regulation of cholesterol metabolism during high fatty acid-induced lipid deposition in calf hepatocytes. [Abstract]

2023 Aug;106(8):5835-5852. PMID: 37419743
Antiviral Res

Targeting 7-dehydrocholesterol reductase against EV-A71 replication by upregulating interferon response. [Abstract]

2023 Jan:209:105497. PMID: 36528172

U18666A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]

Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.
J Virol

The cholesterol transporter Niemann-Pick C1 facilitates the entry of porcine epidemic diarrhea coronavirus. [Abstract]

2026 Jun 9:e0030126. PMID: 42262129
BMC Cancer

APOL4-mediated intracellular cholesterol trafficking is essential for glioblastoma cell growth. [Abstract]

2025 May 21;25(1):906. PMID: 40399861
J Virol

Bombyx mori Nucleopolyhedrovirus Hijacks Multivesicular Body as an Alternative Envelopment Platform for Budded Virus Egress. [Abstract]

2023 Mar 30;97(3):e0004123. PMID: 36916914
J Virol

Interferon-Induced Transmembrane Protein 3 Is a Virus-Associated Protein Which Suppresses Porcine Reproductive and Respiratory Syndrome Virus Replication by Blocking Viral Membrane Fusion. [Abstract]

2020 Nov 23;94(24):e01350-20. PMID: 32999030
J Virol

The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance of Stress Granules by Promoting Amphisome Formation. [Abstract]

2020 Mar 17;94(7):e01719-19. PMID: 31969431
Vet Res

The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation. [Abstract]

2025 Jan 31;56(1):23. PMID: 39891192
PLoS Negl Trop Dis

Identification of anti-flaviviral drugs with mosquitocidal and anti-Zika virus activity in Aedes aegypti. [Abstract]

2019 Aug 20;13(8):e0007681. PMID: 31430351
Vet Microbiol

Cholesterol-dependent Nsp5-endosomes co-trafficking to lysosomes facilitates porcine reproductive and respiratory syndrome virus replication by activating autophagy. [Abstract]

2025 Jun:305:110507. PMID: 40215803
Vet Microbiol

25-hydroxycholesterol inhibits classical swine fever virus entry into porcine alveolar macrophages by depleting plasma membrane cholesterol. [Abstract]

2023 Mar:278:109668. PMID: 36709687
bioRxiv

2026 Jun 18.
bioRxiv

Drug-induced phospholipidosis as an artifact in antiviral drug repurposing. [Abstract]

2025 Nov 21:2025.11.20.689520. PMID: 41332603
bioRxiv

2024 October 17.
Research Square Preprint

2023 Jun 22.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 20.83 mg/mL (49.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5.56 mg/mL (13.11 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3582 mL

11.7908 mL

23.5816 mL

5 mM

0.4716 mL

2.3582 mL

4.7163 mL

10 mM

0.2358 mL

1.1791 mL

2.3582 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 5 mg/mL (11.79 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Poh MK, et al. U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication. Antiviral Res. 2012 Jan;93(1):191-8. [Content Brief]

[2]. Cenedella RJ, et al. Cholesterol synthesis inhibitor U18666A and the role of sterol metabolism and trafficking in numerous pathophysiological processes. Lipids. 2009 Jun;44(6):477-87. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.3582 mL	11.7908 mL	23.5816 mL	58.9539 mL
	5 mM	0.4716 mL	2.3582 mL	4.7163 mL	11.7908 mL
	10 mM	0.2358 mL	1.1791 mL	2.3582 mL	5.8954 mL
DMSO	15 mM	0.1572 mL	0.7861 mL	1.5721 mL	3.9303 mL
	20 mM	0.1179 mL	0.5895 mL	1.1791 mL	2.9477 mL
	25 mM	0.0943 mL	0.4716 mL	0.9433 mL	2.3582 mL
	30 mM	0.0786 mL	0.3930 mL	0.7861 mL	1.9651 mL
	40 mM	0.0590 mL	0.2948 mL	0.5895 mL	1.4738 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

U18666A

29 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

U18666A

Anti-Infection Compound Library

Antiviral Compound Library

Lipid Compound Library

Chemical Probe Library

Anti-Hepatitis C Virus Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) U18666A

Biologische Aktivität

Chemical Information

Publications (29)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

U18666A

Anti-Infection Compound Library

Antiviral Compound Library

Lipid Compound Library

Chemical Probe Library

Anti-Hepatitis C Virus Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) U18666A

Biologische Aktivität

Chemical Information

Publications (29)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

U18666A Related Classifications

HY-107433 Related Classifications