Amsonic acid

CD Sprague-Dawley Rat (female, weanling)^[1]

0.1 mg/kg; 1 mg/kg; 10 mg/kg; 30 mg/kg; 100 mg/kg; 300 mg/kg; 1000 mg/kg; 3000 mg/kg (i.p.); 1000 mg/kg; 3000 mg/kg (p.o.)

i.p.; single dose; p.o.; single dose

Caused no change in relative uterine weight at 0.1 and 1 mg/kg i.p..
Produced equivocal effects at 10, 30, and 100 mg/kg i.p., with only the 30 mg/kg dose showing a statistically significant 16% increase in relative uterine weight.
Induced a statistically significant 66% increase in relative uterine weight at 300 mg/kg i.p..
Caused a statistically significant 50% increase in relative uterine weight at 1000 mg/kg i.p., with no overt toxicity observed.
Resulted in toxicity at 3000 mg/kg i.p., with 2 out of 6 animals dying within 24 hours.
Produced distinct uterotropic responses with no toxicity at 1000 and 3000 mg/kg p.o..
Caused a statistically significant increase in relative uterine weight for a workplace sample at 300 mg/kg i.p., consistent with the commercial sample.
Showed a time course of uterine weight gain after 300 mg/kg i.p. similar to that of equiactive 0.01 mg/kg i.p. diethylstilbestrol, with a multiphasic response including an early and late phase.