ML328
Based on 1 Customer Validation
ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection.
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- Reinheit: 98.94%
- CAS. Nr.: 634175-34-1
- Formel: C22H21F3N6O3S
- Molecular Weight:506.50
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
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Helicase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | IC50 |
0.9 μM
Compound: 11
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Inhibition of human autotaxin isolated from MDA-MB-231 cells assessed as blockade of FS3 substrate hydrolysis by FRET assay
Inhibition of human autotaxin isolated from MDA-MB-231 cells assessed as blockade of FS3 substrate hydrolysis by FRET assay
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[PMID: 20041668] |
| Sf9 | IC50 |
900 μM
Compound: Hoeglund 11
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Inhibition of C-terminal FLAG tagged human recombinant ATX expressed in insect sf9 cells using FS-3 as substrate measured every 2 mins for 60 mins by FRET assay
Inhibition of C-terminal FLAG tagged human recombinant ATX expressed in insect sf9 cells using FS-3 as substrate measured every 2 mins for 60 mins by FRET assay
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[PMID: 27544588] |
ML328 (0.1-1000 μM) shows AddAB and RecBCD nuclease inhibitory activities with IC50 values of 26 and 5.1 μM, respectively[1]. ML328 pathway-specifically inhibits of (high-frequency recombination) Hfr for RecBCD, RecF and RecE pathways[1]. ML328 shows RecBCD inhibitory effects with IC50 values of 3 μΜ for nuclease and Chi-cutting with purified enzyme, 0.3 μΜ for Hfr recombination and 5 μΜ for phage λ recombination promoted by RecBCD[2]. ML328 inhibits E. coli RecBCD, H. pylori AddAB, M. smeg AddAB and M. smeg RecBCD with IC50 values of 4.6, 16, 2.4 and 5.5 μM, respectively[2]. ML328 (25 μΜ; 2 h) reduces the frequency of H2O2-induced mutation in E. coli[2]. ML328 (25 μΜ; 1 h) reduces the requency of H2O2-induced mutation to valine-resistance (valineR) in E. coli[2]. ML328 (50 μΜ) slightly inhibits E. coli growth but strongly inhibits E. coli at the presence of phage[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 634175-34-1
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Appearance Solid
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Molecular Weight 506.50
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Formel C22H21F3N6O3S
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Color White to off-white
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SMILES
O=C(C1=CN(CC)C2=NC(N3CCN(C(NC4=CC=CC(C(F)(F)F)=C4)=S)CC3)=NC=C2C1=O)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 25 mg/mL (49.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9743 mL | 9.8717 mL | 19.7433 mL | 49.3583 mL |
| 5 mM | 0.3949 mL | 1.9743 mL | 3.9487 mL | 9.8717 mL | |
| 10 mM | 0.1974 mL | 0.9872 mL | 1.9743 mL | 4.9358 mL | |
| 15 mM | 0.1316 mL | 0.6581 mL | 1.3162 mL | 3.2906 mL | |
| 20 mM | 0.0987 mL | 0.4936 mL | 0.9872 mL | 2.4679 mL | |
| 25 mM | 0.0790 mL | 0.3949 mL | 0.7897 mL | 1.9743 mL | |
| 30 mM | 0.0658 mL | 0.3291 mL | 0.6581 mL | 1.6453 mL | |
| 40 mM | 0.0494 mL | 0.2468 mL | 0.4936 mL | 1.2340 mL |
ML328 Related Classifications
HY-136638 Related Classifications
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