Art. -Nr.: HY-118487

OB-24

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

OB-24 is a selective small-molecule HO-1 inhibitor (IC₅₀ = 1.9 μM for HO-1 and IC₅₀ for HO-2 >100 μM). OB-24 possesses anti-tumor and anti-metastatic properties. OB-24 can be studies in research such as prostate cancer, melanoma, ovarian carcinoma and lung metastasis.

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Reinheit: 98.21%
CAS. Nr.: 939825-12-4
Formel: C₁₅H₁₈BrClN₂O₂
Molecular Weight:373.67

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

OB-24 Chemical Structure

CAS. Nr.: 939825-12-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target_[1]

HO-1

1.9 μM (IC₅₀)

HO-2

>100 μM (IC₅₀)

Alle Heme Oxygenase (HO) Isoform-spezifische Produkte anzeigen :

HO-1
HO-2

In Vitro

OB-24 (10 μM, 24-48 h) results in around 20% growth inhibition and 62% HO-1 inhibition in PC3M cells^[1].
OB-24 (6.5 μM) reduces cell proliferation by approximately 10%, HO-1 activity by 59%, protein carbonylation by 40%, and intracellular ROS by 43% in HO-1 overexpressing rat glioma C6 cells^[1].
OB-24 (10 μM, 96 h) induces around 86% protein carbonylation inhibition, and 25% intracellular ROS inhibition in PC3M cells^[1].
OB-24 (5-10 μM, 10 min) reduces activation of the MAPK ERK and p38 kinases but no clear inhibition is seen on JNK or AKT phosphorylation in PC3M cells and results in similar inhibition in LNCaP and DU145 cells^[1]. OB-24 has selectivity to HO-1 (IC50 = 1.9 μM against HO-1 in rat spleen) whereas the IC50 against HO-2 in rat brain is >100 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

OB-24 (10-60 mg/kg, i.p., on days 1, 3, and 5 per cycle for 4 cycle) results in a dose-dependent inhibition of tumor growth and no body weight loss in PC3M Scid male mice^[1].
OB-24 (10-30 mg/kg, i.v., on days 1, 3, and 5 per cycle for 4 cycle) results in approximately 50% tumor growth reduction and lymph node and lung metastases with 30 mg/kg in PC3M Scid male mice bearing palpable advanced prostate cancer^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

PC3M Scid male mice bearing palpable PCA^[1]

Dosage:

10-30 mg/kg, on days 1, 3, and 5 per cycle for 4 cycle

Administration:

Intravenous injection (i.v.)

Result:

Resulted in approximately 50% tumor growth reduction and lymph node and lung metastases with 30 mg/kg dosage.
Had less effect with 10 mg/kg dosage.
Exceeded 90% inhibitory effects when combined with Taxol (10 mg/kg).

Chemical Information

CAS. Nr. 939825-12-4

Appearance Solid

Molecular Weight 373.67

Formel C₁₅H₁₈BrClN₂O₂

Color Off-white to light yellow

SMILES

BrC1=CC=C(CCC2(CN3C=CN=C3)OCCO2)C=C1.Cl

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (2)

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Journal Impact Factor

Most Recent

Chem Biol Interact

Oridonin exposure induces testicular impairment through regulating ferroptosis via HIF-1α/HO-1 activation and STUB1-driven GPX4 ubiquitination-dependent degradation. [Abstract]

2026 Jan 5:423:111838. PMID: 41265811

bioRxiv

Myeloid progenitor dysregulation fuels immunosuppressive macrophages in tumors. [Abstract]

2024 Jun 28:2024.06.24.600383. PMID: 38979166

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (267.62 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6762 mL

13.3808 mL

26.7616 mL

5 mM

0.5352 mL

2.6762 mL

5.3523 mL

10 mM

0.2676 mL

1.3381 mL

2.6762 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

=
Konzentration Konzentration

×
Volumen Volumen

×
Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

×

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

=

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

×

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO    40% PEG300    5% Tween-80    45% Saline
Solubility: 2.5 mg/mL (6.69 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Protocol 2

Add each solvent one by one: 10% DMSO    90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.69 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

Protocol 3

Add each solvent one by one: 10% DMSO    90% Corn Oil
Solubility: 2.5 mg/mL (6.69 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)

SDS (252 KB)

English - EN (252 KB)

Français - FR (252 KB)

Deutsch - DE (252 KB)

Norwegian - NO (252 KB)

Español - ES (252 KB)

Swedish - SV (252 KB)

Italian - IT (252 KB)

Korean - KR (252 KB)

Portuguese - PT (252 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Alaoui-Jamali MA, et al. A novel experimental heme oxygenase-1-targeted therapy for hormone-refractory prostate cancer. Cancer Res. 2009;69(20):8017-8024. [Content Brief]

[2]. Moulay. Alaoui-Jamali, et al., OB-24, a novel selective and potent HO-1 inhibitor, induces a wide spectrum anti-tumor activity in vitro and in vivo and synergizes with chemotherapy drugs. Mol Cancer Ther 1 November 2007; 6 (11_Supplement): C82.

[3]. Roman, G., et al., (2007). Heme oxygenase inhibition by 2-oxy-substituted 1-(1H-imidazol-1-yl)-4-phenylbutanes: effect of halogen substitution in the phenyl ring. Bioorganic & medicinal chemistry, 15(9), 3225–3234. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 2.6762 mL 13.3808 mL 26.7616 mL 66.9040 mL

5 mM 0.5352 mL 2.6762 mL 5.3523 mL 13.3808 mL

10 mM 0.2676 mL 1.3381 mL 2.6762 mL 6.6904 mL

15 mM 0.1784 mL 0.8921 mL 1.7841 mL 4.4603 mL

20 mM 0.1338 mL 0.6690 mL 1.3381 mL 3.3452 mL

25 mM 0.1070 mL 0.5352 mL 1.0705 mL 2.6762 mL

30 mM 0.0892 mL 0.4460 mL 0.8921 mL 2.2301 mL

40 mM 0.0669 mL 0.3345 mL 0.6690 mL 1.6726 mL

50 mM 0.0535 mL 0.2676 mL 0.5352 mL 1.3381 mL

60 mM 0.0446 mL 0.2230 mL 0.4460 mL 1.1151 mL

80 mM 0.0335 mL 0.1673 mL 0.3345 mL 0.8363 mL

100 mM 0.0268 mL 0.1338 mL 0.2676 mL 0.6690 mL

OB-24 Related Classifications

HY-118487 Related Classifications

Metabolic Enzyme/Protease NF-κB Immunology/Inflammation

Heme Oxygenase (HO) Reactive Oxygen Species (ROS)

Disease Areas

Cancer Urogenital Disease

Prostate Cancer Urogenital Cancer Ovarian Cancer

Cancer

Cancer Targeted Therapy Cancer Immunotherapy Cancer Metabolism and Metastasis

Powered by Bioz See more details on Bioz

Keywords

OB-24
939825-12-4
OB24
OB 24
Heme Oxygenase (HO)
Reactive Oxygen Species (ROS)
HO-1
heme oxygenase-1
heat shock protein
anti-tumor
anti-metastasis
prostate cancer
ROS
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6762 mL	13.3808 mL	26.7616 mL	66.9040 mL
5 mM	0.5352 mL	2.6762 mL	5.3523 mL	13.3808 mL
10 mM	0.2676 mL	1.3381 mL	2.6762 mL	6.6904 mL
15 mM	0.1784 mL	0.8921 mL	1.7841 mL	4.4603 mL
20 mM	0.1338 mL	0.6690 mL	1.3381 mL	3.3452 mL
25 mM	0.1070 mL	0.5352 mL	1.0705 mL	2.6762 mL
30 mM	0.0892 mL	0.4460 mL	0.8921 mL	2.2301 mL
40 mM	0.0669 mL	0.3345 mL	0.6690 mL	1.6726 mL
50 mM	0.0535 mL	0.2676 mL	0.5352 mL	1.3381 mL
60 mM	0.0446 mL	0.2230 mL	0.4460 mL	1.1151 mL
80 mM	0.0335 mL	0.1673 mL	0.3345 mL	0.8363 mL
100 mM	0.0268 mL	0.1338 mL	0.2676 mL	0.6690 mL

OB-24

2 Cited Publications

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OB-24

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Prostate Cancer Compound Library

Highly Selective Inhibitors Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) OB-24

Alle Heme Oxygenase (HO) Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table