Ozanezumab
Based on 1 Customer Validation
Ozanezumab (GSK1223249) is a humanized, Fc-inactivated monoclonal antibody that targets the nervous system protein Nogo-A. Ozanezumab promotes neurite outgrowth and axonal regeneration by neutralizing Nogo-A signaling. Ozanezumab is used for research on neurodegenerative diseases such as amyotrophic lateral sclerosis and multiple sclerosis[1].
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99%
- CAS. Nr.: 1310680-64-8
- Molecular Weight:145.227 kDa
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Human IgG1 kappa
Human
RTN4/NOGO
Ozanezumab reduces or reverses the inhibitory effect on axonal growth in a dose-dependent manner in cultured cerebellar granule neurons from postnatal rats[2].
Ozanezumab (0.7-100 μg/mL (4.67 to 667 nM); 1 h pre-incubation, 24 h total) dose-dependently neutralizes the inhibitory effect of GST-Nogo-A on axonal growth of cultured postnatal rat cerebellar granule neurons[3].
Ozanezumab exhibits specific immunohistochemical staining in conserved neural tissues, muscle tissues, and some other tissues in Rattus norvegicus (rat), Macaca (monkey), and Oryctolagus cuniculus (rabbit, central nervous system), while additional species-specific staining is present in neuronal tissues, pituitary tissues, and cardiac tissues of rodents and primates[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 kappa
ELISA, FACS, Functional assay
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Flow Cytometry analysis of Hela cells labelling RTN4/NOGO (red) with Ozanezumab (anti-RTN4/NOGO) (HY-P99213). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS. Nr. 1310680-64-8
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Appearance Liquid
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Molecular Weight 145.227 kDa
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Color Colorless to light yellow
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SMILES
[Ozanezumab]
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Synonyms
GSK1223249
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Versand
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
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Data Sheet (258 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Verweise
[1]. Gharagozloo M, et al. Breaking the barriers to remyelination in multiple sclerosis. Curr Opin Pharmacol. 2022;63:102194. [Content Brief]
[2]. Meininger V, et al. Safety and efficacy of ozanezumab in patients with amyotrophic lateral sclerosis: a randomised, double-blind, placebo-controlled, phase 2 trial. Lancet Neurol. 2017;16(3):208-216. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)