Art. -Nr.: HY-171579

RS47

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RS47 is a selective RelB inhibitor with a Kd value of 1.1 μM. RS47 also acts as an inhibitor of HCV replication. RS47 can block the non-canonical NF-κB signaling pathway without affecting the canonical pathway. RS47 exerts anti-tumor effects of inhibiting proliferation and promoting apoptosis on colorectal cancer, B-cell lymphoma and other related tumors both in vitro and in vivo by disrupting the binding of RelB to target DNA. RS47 can be used for the research of tumors and infectious diseases.

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Reinheit: 98.0%
CAS. Nr.: 301655-16-3
Formel: C₁₈H₁₈N₄O₂S
Molecular Weight:354.43

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

RS47 Chemical Structure

CAS. Nr.: 301655-16-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Alle Pim Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-5-2	CC₅₀	>141 μM Compound: 1a-SPECS	Cytotoxicity against human HuH5.2 cells assessed as reduction in metabolic activity after 72 hrs by MTS assay Cytotoxicity against human HuH5.2 cells assessed as reduction in metabolic activity after 72 hrs by MTS assay	[PMID: 27474921]
Huh-5-2	CC₅₀	>282 μM Compound: 1a-synth	Cytotoxicity against human HuH5.2 cells assessed as reduction in metabolic activity after 72 hrs by MTS assay Cytotoxicity against human HuH5.2 cells assessed as reduction in metabolic activity after 72 hrs by MTS assay	[PMID: 27474921]

In Vitro

RS47 (0-10 μM) inhibits HCT116, BJAB and U2932 cells viability with IC₅₀ values of 1.28 , 0.255 and 0.159 μM^[1].
RS47 (0-3 μM; 0-7 days) can inhibit the proliferation, colony formation, and invasion of HCT116 cells^[1].
RS47 (0-2 μM; 72 h) induces apoptosis and cell cycle arrest in HCT116/SW620 colorectal cancer cells^[1].
RS47 (0-2 μM; 72 h) promotes apoptosis in BJAB and U2932 cells^[1].
RS47 (1 μM; 24 h) inhibits the expression of BAFF-inducible target genes (e.g., Pim2) in the non-canonical NF-κB pathway in BJAB cells and primary B cells^[1].
RS47 inhibits HCV replication in Huh5-2 cells (EC₅₀ < 2.2 μM)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

HCT116, SW620
Concentration:

0.5 μM, 1 μM, 2 μM
Incubation Time:

72 h
Result:

Caused a significant decrease in S phase population and an increase in G0/G1 and G2/M phase populations in a dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

BJAB, U2932
Concentration:

0 μM, 0.5 μM, 1 μM, 2 μM
Incubation Time:

72 h
Result:

Significantly promoted apoptosis in a dose-dependent manner.

Real Time qPCR^[1]

Cell Line:

BJAB, primary B cells
Concentration:

1 μM
Incubation Time:

24 h
Result:

Inhibited BAFF-induced Pim2 mRNA expression in BJAB cells and primary B cells.

In Vivo

RS47 (50 mg/kg; i.p.; 21 days) inhibits tumor growth of human B lymphoma PDX xenografts in BALB/c athymic nude mice^[1].
RS47 (50 mg/kg; i.p.; 21 days) significantly inhibits tumor growth and downregulates c-Myc expression in BALB/c nude mice with colorectal cancer xenografts^[1]。

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c athymic nude mice (male, 6-week-old, induced by subcutaneous injection of human B lymphoma tissue from PDX models)^[1]
Dosage:

50 mg/kg
Administration:

i.p.; daily
Result:

Remarkably reduced the tumor volume and weight of B lymphoma PDX xenografts. Immunohistochemical staining showed a decreased percentage of Ki-67-positive proliferating cells, and TUNEL assay revealed an increased number of apoptotic cells in the tumor tissues.
Downregulated the mRNA and protein expression of Bcl-2 and MYC, which are associated with cell survival and proliferation.
Had no/little toxicity to mice.

Chemical Information

CAS. Nr. 301655-16-3
Appearance Solid
Molecular Weight 354.43
Formel C₁₈H₁₈N₄O₂S
Color Yellow to brown
SMILES

OC1=CC=C(C(/C(C)=N/NC2=C3C(CCCC4)=C4SC3=NC=N2)=C1)O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (70.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8214 mL

14.1072 mL

28.2143 mL

5 mM

0.5643 mL

2.8214 mL

5.6429 mL

10 mM

0.2821 mL

1.4107 mL

2.8214 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Li C, et al. Development of RelB-targeting small-molecule inhibitors of non-canonical NF-κB signaling with antitumor efficacy. Mol Ther. 2025;33(4):1519-1534. [Content Brief]

[2]. Bassetto M, et al. Computer-aided identification, synthesis and evaluation of substituted thienopyrimidines as novel inhibitors of HCV replication. Eur J Med Chem. 2016;123:31-47. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8214 mL	14.1072 mL	28.2143 mL	70.5358 mL
	5 mM	0.5643 mL	2.8214 mL	5.6429 mL	14.1072 mL
	10 mM	0.2821 mL	1.4107 mL	2.8214 mL	7.0536 mL
	15 mM	0.1881 mL	0.9405 mL	1.8810 mL	4.7024 mL
	20 mM	0.1411 mL	0.7054 mL	1.4107 mL	3.5268 mL
	25 mM	0.1129 mL	0.5643 mL	1.1286 mL	2.8214 mL
	30 mM	0.0940 mL	0.4702 mL	0.9405 mL	2.3512 mL
	40 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7634 mL
	50 mM	0.0564 mL	0.2821 mL	0.5643 mL	1.4107 mL
	60 mM	0.0470 mL	0.2351 mL	0.4702 mL	1.1756 mL

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RS47

Anti-Infection Compound Library

Apoptosis Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Antiviral Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Glutamine Metabolism Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle NF-κB Isoform-spezifische Produkte anzeigen

Alle Pim Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RS47 Related Classifications

HY-171579 Related Classifications