ABCA1 inducer 2
Based on 1 Customer Validation
ABCA1 inducer 2 is a non-lipotropic ABCA1 inducer. ABCA1 inducer 2 upregulates the expression of ABCA1 by targeting the LXR pathway. ABCA1 inducer 2 can reduce ox-LDL-induced lipid accumulation and thus inhibit foam cell formation. ABCA1 inducer 2 has anti-atherosclerotic potential.
For research use only. We do not sell to patients.
- Purity : 98.22%
- CAS No.: 2451589-46-9
- Formula: C16H11BrN2O
- Molecular Weight:327.18
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
ABCA1 inducer 2 (Compound 3) (0.01-10 μM, 24 h) has a strong ability to activate the ABCA1 promoter in Hela cells[1].
ABCA1 inducer 2 (0.01-10 μM, 18 h) effectively induces the expression of ABCA1 mRNA in RAW 264.7 cells in a dose-dependent manner, with the maximum increase at 10 μM being 3.94-fold of that in the control group[1].
ABCA1 inducer 2 (0.01-10 μM, 12-48 h) upregulates ABCA1 protein expression in RAW 264.7 cells and Hela cells by acting on the LXR pathway[1].
ABCA1 inducer 2 (10 μM, 24 h) can effectively reduce ox-LDL-induced intracellular lipid accumulation and inhibit the formation of foam cells in RAW 264.7 cells[1].
ABCA1 inducer 2 (10 μM, 48 h) induces limited lipid and triglyceride accumulation in HepG2 cells, reduces adverse effects associated with hypertriglyceridemia and hepatic lipogenesis[1].
ABCA1 inducer 2 (0.01-10 μM, 24 h) has no significant toxicity to Hela, RAW264.7 and HepG2 cells at a concentration of 10 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 cells
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Concentration:0.01-10 μM
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Incubation Time:12-48 h
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Result:At a concentration of 10 μM, ABCA1 protein expression in RAW 264.7 cells increased, with a maximum response time of 24 to 48 hours.
Significantly upregulated ABCA1 expression in a dose-dependent manner within the concentration range of 0.01 to 10 μM.
Completely depended on the LXR pathway for the activation of ABCA1, as GSK2033 completely blocked this effect.
Chemical Information
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CAS No. 2451589-46-9
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Appearance Solid
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Molecular Weight 327.18
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Formula C16H11BrN2O
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Color Off-white to light yellow
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SMILES
O=C1N(CCC2=C3C=CC=C2)C3=NC4=C1C(Br)=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
DMSO : 5.88 mg/mL (17.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.59 mg/mL (1.80 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 0.59 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0564 mL | 15.2821 mL | 30.5642 mL | 76.4105 mL |
| 5 mM | 0.6113 mL | 3.0564 mL | 6.1128 mL | 15.2821 mL | |
| 10 mM | 0.3056 mL | 1.5282 mL | 3.0564 mL | 7.6411 mL | |
| 15 mM | 0.2038 mL | 1.0188 mL | 2.0376 mL | 5.0940 mL |
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