Cat. No.: HY-N1382

Asperuloside

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Asperuloside is an iridoid isolated from Hedyotis diffusa, with anti-inflammatory activity. Asperuloside inhibits inducible nitric oxide synthase (iNOS), suppresses NF-κB and MAPK signaling pathways.

For research use only. We do not sell to patients.

Purity: 99.56%
CAS No.: 14259-45-1
Formula: C₁₈H₂₂O₁₁
Molecular Weight:414.36

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Asperuloside Chemical Structure

CAS No.: 14259-45-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

iNOS

All NO Synthase Isoforms :

In Vitro

Asperuloside (0-160 μg/mL, 1 h) inhibits productions of NO, PGE2, TNF-α and IL-6 in LPS-Induced RAW 264.7 cells^[1].
Asperuloside (0-5 mM, 24 h) inhibits cell viability and induces ER stress dependent apoptosis in leukemia cell lines (U937, HL-60, AML)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

U937 cells
Concentration:

0-5 mM
Incubation Time:

24 h
Result:

Increased the cleaved Caspase-3, Caspase-9, and PARP, and Cyto-c release from mitochondria.
Induced expression of GRP78, p-PERK, p-eIF2α, CHOP, p-IRE1, XBP1, ATF6 and cleaved Caspase-12

In Vivo

Asperuloside (p.o., 3 mg/day, 0.3% in diet, daily, 12 weeks) reduces food intake, body weight, and adipose masses in rats consuming a high fat diet (HFD)^[2].
Asperuloside (30 and 60 mg/kg, i.p., 30 days) inhibits tumor growth of U937 xenografts mice model^[3].
Asperuloside (20-80 mg/kg, i.p.) relieves LPS-induced acute lung injury via inhibiting MAPK and NF-κB signaling in BALB/c mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Rats consuming a high fat diet (HFD)^[2]
Dosage:

3 mg/day
Administration:

p.o., 0.3% in diet, daily, 12 weeks
Result:

Reduced body weight, energy intake, adiposity, blood glucose, and plasma insulin.

Chemical Information

CAS No. 14259-45-1
Appearance Solid
Molecular Weight 414.36
Formula C₁₈H₂₂O₁₁
Color Off-white to light yellow
SMILES

O=C1O[C@]2([H])[C@@]3([H])[C@](C(COC(C)=O)=C2)([H])[C@H](O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)OC=C13
Structure Classification
- Terpenoids
- Iridoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

Acta Pharm Sin B

2026 May 21.
J Ethnopharmacol

Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. [Abstract]

2022 Jan 30:283:114739. PMID: 34648903
Molecules

Phytochemical Screening and Antioxidant Activity of Selected Estonian Galium Species. [Abstract]

2023 Mar 22;28(6):2867. PMID: 36985838

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (241.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (60.33 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4134 mL

12.0668 mL

24.1336 mL

5 mM

0.4827 mL

2.4134 mL

4.8267 mL

10 mM

0.2413 mL

1.2067 mL

2.4134 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. He J, et al. Asperuloside and Asperulosidic Acid Exert an Anti-Inflammatory Effect via Suppression of the NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages. Int J Mol Sci. 2018 Jul 12;19(7). [Content Brief]

[2]. Ishaq M, et al. Asperuloside Enhances Taste Perception and Prevents Weight Gain in High-Fat Fed Mice. Front Endocrinol (Lausanne). 2021 Apr 13;12:615446. [Content Brief]

[3]. Rong C, et al. Asperuloside exhibits a novel anti-leukemic activity by triggering ER stress-regulated apoptosis via targeting GRP78. Biomed Pharmacother. 2020 May;125:109819. [Content Brief]

[4]. Qiu J, et al. Pretreatment with the compound asperuloside decreases acute lung injury via inhibiting MAPK and NF-κB signaling in a murine model. Int Immunopharmacol. 2016 Feb;31:109-15. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.4134 mL	12.0668 mL	24.1336 mL	60.3340 mL
	5 mM	0.4827 mL	2.4134 mL	4.8267 mL	12.0668 mL
	10 mM	0.2413 mL	1.2067 mL	2.4134 mL	6.0334 mL
	15 mM	0.1609 mL	0.8045 mL	1.6089 mL	4.0223 mL
	20 mM	0.1207 mL	0.6033 mL	1.2067 mL	3.0167 mL
	25 mM	0.0965 mL	0.4827 mL	0.9653 mL	2.4134 mL
	30 mM	0.0804 mL	0.4022 mL	0.8045 mL	2.0111 mL
	40 mM	0.0603 mL	0.3017 mL	0.6033 mL	1.5084 mL
	50 mM	0.0483 mL	0.2413 mL	0.4827 mL	1.2067 mL
	60 mM	0.0402 mL	0.2011 mL	0.4022 mL	1.0056 mL
DMSO	80 mM	0.0302 mL	0.1508 mL	0.3017 mL	0.7542 mL
DMSO	100 mM	0.0241 mL	0.1207 mL	0.2413 mL	0.6033 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Asperuloside

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Publications Citing Use of MedChemExpress (MCE) Asperuloside

All NO Synthase Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Asperuloside Related Classifications

HY-N1382 Related Classifications