Azoxymethane
Based on 17 publication(s) in Google Scholar
Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.
For research use only. We do not sell to patients.
- Purity: 99.45%
- CAS No.: 25843-45-2
- Formula: C2H6N2O
- Molecular Weight:74.08
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Storage:
Solution, -20°C, 2 years
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Azoxymethane
More- Cancer Commun (Lond). 2025 Oct 16. [Abstract]
- Nat Chem Biol. 2025 Jan;21(1):59-70. [Abstract]
- Adv Sci (Weinh). 2025 May 8:e2500271. [Abstract]
- Cell Commun Signal. 2026 Mar 23;24(1):259. [Abstract]
- Phytomedicine. 2024 Jan:122:155095. [Abstract]
- EBioMedicine. 2020 Nov;61:103068. [Abstract]
- J Transl Med. 2024 Nov 15;22(1):1028. [Abstract]
- Free Radic Biol Med. 2021 Aug 20:172:312-329. [Abstract]
- Clin Transl Med. 2024 Jan;14(1):e1535. [Abstract]
- Int Immunopharmacol. 2024 May 27:135:112262. [Abstract]
- Int Immunopharmacol. 2024 Jan 5:126:111188. [Abstract]
- Probiotics Antimicrob Proteins. 2025 Nov 26. [Abstract]
- mSystems. 2025 Jan 22:e0156724. [Abstract]
- Sci Rep. 2024 Aug 5;14(1):18094. [Abstract]
- J Radiat Res Appl Sci. December 2022, 100470.
- Biochem Biophys Res Commun. 2025 Sep 8:778:152396. [Abstract]
- Asia Pac J Clin Oncol. 2023 Oct;19(5):e248-e257. [Abstract]
Customer Validation & Images
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Histological Imaging/Staining
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Histological Imaging/Staining
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IHC
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IHC
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Bio/Physico-chemical Assay
Biological Activity
Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts. On an equal protein basis, hepatic microsomes are much more active than SI and colon microsomes in NADPH-dependent Azoxymethane bioactivation and N7-mG adduct formation. Hepatic microsomes show the highest activity in the hydroxylation of Azoxymethane, followed by SI and colon microsomes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
Azoxymethane can be used to induce colorectal cancer models.
Administration: 5 mg/kg• i.p.• weekly for 6 weeks
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 25843-45-2
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Appearance Liquid
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Molecular Weight 74.08
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Formula C2H6N2O
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Color Colorless to light yellow
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SMILES
C/[N+]([O-])=N/C
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Synonyms
AOM
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Publications (17)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic target. [Abstract]2025 Oct 16. PMID: 41103012 -
Nat Chem Biol
2025 Jan;21(1):59-70. PMID: 39039255 -
Adv Sci (Weinh)
PRMT5 Inhibitor Synergizes with Chemotherapy to Induce Resembling Mismatch Repair Deficiency and Enhance Anti-TIGIT Therapy in Microsatellite-Stable Colorectal Cancer. [Abstract]2025 May 8:e2500271. PMID: 40344511
Azoxymethane purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 8:e2500271. [Abstract]
H&E staining in AOM (10 mg/kg; sc; Seven days later, 2.5% dextran sulfate sodium, was administered in the drinking water for 7 days, followed by 2 weeks of regular drinking water, This DSS treatment cycle was repeated twice for a total of three cycles)/DSS‐induced tumor‐bearing mice treated with DMSO, PRMT5i (40 mg kg−1), CPT‐11 (40 mg kg−1), or PRMT5i + CPT‐11.
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Cell Commun Signal
Atorvastatin promotes lipid catabolism in colorectal cancer via FDFT1‒mediated inhibition of the PI3K/AKT pathway. [Abstract]2026 Mar 23;24(1):259. PMID: 41866501 -
Phytomedicine
Ochratoxin A promotes chronic enteritis and early colorectal cancer progression by targeting Rinck signaling. [Abstract]2024 Jan:122:155095. PMID: 37844381 -
EBioMedicine
2020 Nov;61:103068. PMID: 33099085
Azoxymethane purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2020 Nov;61:103068. [Abstract]
HE staining of normal epithelium and AOM-DSS (a single dose of AOM (12 mg/kg); ip; fter 3 days, 2% DSS dissolved in the drinking water was administered for 6 days, followed by 15 days of regular drinking water) induced colorectal carcinoma in WT and Ndrg2DIEC mice. Representative images from WT (n=24) and Ndrg2DIEC (n=26) mice.
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J Transl Med
Gut microbial and metabolomics profiles reveal the potential mechanism of fecal microbiota transplantation in modulating the progression of colitis-associated colorectal cancer in mice. [Abstract]2024 Nov 15;22(1):1028. PMID: 39548468 -
Free Radic Biol Med
Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis. [Abstract]2021 Aug 20:172:312-329. PMID: 34144192
Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329. [Abstract]
Immunohistochemical staining showing the histological changes in colon tissues upon OTSSP167 treatment in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.
Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329. [Abstract]
Immunohistochemistry revealed the expression of ferroptosis related indicators, including 4HNE and MPO treated in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice
Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329. [Abstract]
Differences in the expression of COX2 and SLC39A14 in colon tissues treated with or without OTSSP167 in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.
Azoxymethane purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2021 Aug 20:172:312-329. [Abstract]
Changes in the secretion of proinflammatory factors, including IL-1β, IL-6 and TNF-α , induced by the OTSSP167 treatment in AOM/DSS (injected intraperitoneally with 10 mg/kg AOM on day 0, and then, on the 7th day after the administration of AOM, the mice were provided water containing 2.5% DSS for three cycles. Each cycle lasted for 5 days, followed by the administration of normal water for 14 days. )-induced CRC mice.
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Clin Transl Med
Single-cell landscape of the cellular microenvironment in three different colonic polyp subtypes in children. [Abstract]2024 Jan;14(1):e1535. PMID: 38264936 -
Int Immunopharmacol
The Glabridin from Huangqin Decoction Prevents the Development of Ulcerative Colitis into Colitis-Associated Colorectal Cancer by Modulating MMP1/MMP3 Activity. [Abstract]2024 May 27:135:112262. PMID: 38805906 -
Int Immunopharmacol
Arabinose confers protection against intestinal injury by improving integrity of intestinal mucosal barrier. [Abstract]2024 Jan 5:126:111188. PMID: 37995573 -
Probiotics Antimicrob Proteins
Potential Benefits of Metformin Via Roseburia Intestinalis in High-fat Diet-fed Mice With Colorectal Adenomas. [Abstract]2025 Nov 26. PMID: 41296177 -
mSystems
The combination of Clostridium butyricum and Akkermansia muciniphila mitigates DSS-induced colitis and attenuates colitis-associated tumorigenesis by modulating gut microbiota and reducing CD8+ T cells in mice. [Abstract]2025 Jan 22:e0156724. PMID: 39840995 -
Sci Rep
Identifying polyamine related biomarkers in diagnosis and treatment of ulcerative colitis by integrating bulk and single-cell sequencing data. [Abstract]2024 Aug 5;14(1):18094. PMID: 39103474 -
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Biochem Biophys Res Commun
2025 Sep 8:778:152396. PMID: 40706248 -
Asia Pac J Clin Oncol
Polysaccharide of Lactobacillus casei SB27 reduced colon cancer cell prognosis through mitochondrial damage by upregulation of HINT2. [Abstract]2023 Oct;19(5):e248-e257. PMID: 36271660
Solvent & Solubility
H2O : 138.89 mg/mL (1874.87 mM; Need ultrasonic)
Protocol
The assay for Azoxymethane-induced in vitro DNA adduct formation is performed. Briefly, microsomes (0.5 to 2.0 mg/mL) are incubated with calf thymus DNA (1 mg/mL) and Azoxymethane (200 μM) in a total volume of 1.0 mL. The assay buffer consists of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations are carried out at 37°C for 60 min in a shaking water bath. An additional 30 nM of NADPH is added after the first 30 min. The reaction is stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA is then extracted for tissue homogenates. Control incubations are performed without NADPH[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male, 8 to 10 week old, WT-A/J, IECN-A/J, and LCN-A/J mice (8 per group) are treated with either saline or Azoxymethane (7.5 mg/kg BW, s.c.), once weekly for 3 weeks. Mice are sacrificed 6 weeks post-treatment for aberrant crypt foci (ACF) detection. The entire colon is excised. A longitudinal incision is made along the entire length of the colon, which is further cut into two equal-length segments, representing proximal and distal portions of the colon. The segments are dipped in PBS to remove fecal pellets and then kept flat between filter papers in 10% buffered formalin for at least 24 h. Subsequently, the colons are immersed in freshly prepared 0.1% methylene blue for 10 min and rinsed briefly in deionized H2O to remove excess dye. The colon is mounted carefully on a microscope slide with the mucosal surface side up and viewed under a light microscope. The ACF in the entire mucosal surface of the colon are counted blindly and independently by two investigators and recorded[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (795 KB)
- English - EN (795 KB)
- Français - FR (795 KB)
- Deutsch - DE (795 KB)
- Norwegian - NO (795 KB)
- Español - ES (795 KB)
- Swedish - SV (795 KB)
- Italian - IT (795 KB)
- Korean - KR (795 KB)
- Portuguese - PT (795 KB)
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Handling Instructions (2659 KB)
References
[1]. Megaraj V, et al. Role of hepatic and intestinal p450 enzymes in the metabolic activation of the colon carcinogen azoxymethane in mice. Chem Res Toxicol. 2014 Apr 21;27(4):656-62. [Content Brief]
[2]. Whetstone RD, et al. Colon carcinogenesis in wild type and immune compromised mice after treatment with azoxymethane, and azoxymethane with dextran sodium sulfate. Mol Carcinog. 2016 Jul;55(7):1187-95. [Content Brief]
[3]. Fichera A, et al. Epidermal growth factor receptor signaling is required for microadenoma formation in the mouse azoxymethane model of colonic carcinogenesis. Cancer Res. 2007 Jan 15;67(2):827-35. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 13.4989 mL | 67.4946 mL | 134.9892 mL | 337.4730 mL |
| 5 mM | 2.6998 mL | 13.4989 mL | 26.9978 mL | 67.4946 mL | |
| 10 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL | 33.7473 mL | |
| 15 mM | 0.8999 mL | 4.4996 mL | 8.9993 mL | 22.4982 mL | |
| 20 mM | 0.6749 mL | 3.3747 mL | 6.7495 mL | 16.8736 mL | |
| 25 mM | 0.5400 mL | 2.6998 mL | 5.3996 mL | 13.4989 mL | |
| 30 mM | 0.4500 mL | 2.2498 mL | 4.4996 mL | 11.2491 mL | |
| 40 mM | 0.3375 mL | 1.6874 mL | 3.3747 mL | 8.4368 mL | |
| 50 mM | 0.2700 mL | 1.3499 mL | 2.6998 mL | 6.7495 mL | |
| 60 mM | 0.2250 mL | 1.1249 mL | 2.2498 mL | 5.6245 mL | |
| 80 mM | 0.1687 mL | 0.8437 mL | 1.6874 mL | 4.2184 mL | |
| 100 mM | 0.1350 mL | 0.6749 mL | 1.3499 mL | 3.3747 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Azoxymethane Related Classifications
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