country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-190838

Copy Link Print Information

AV22

AV22 is a PDIA3 inhibitor that binds to human PDIA3 with a K_d of 1.69 µM and exhibits an IC₅₀ of 7.0 µM against human PDIA3. AV22 has an IC₅₀ of 19.4 µM against human PDIA1. AV22 inhibits the replication of multiple enveloped human respiratory viruses. AV22 can be used for research on respiratory virus infections.

For research use only. We do not sell to patients.

Formula: C₂₂H₁₃Cl₂F₃N₂O₂
Molecular Weight:465.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (281 KB)

AV22 Chemical Structure

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

PDIA3

1.69 μM (Kd)

PDIA3

7 μM (IC₅₀)

PDIA1

19.4 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-8	CC₅₀	31.75 μM	Cytotoxicity against human HCT-8 cells assessed as viability reduction incubated 72 hrs by CellTiter-Glo assay. Cytotoxicity against human HCT-8 cells assessed as viability reduction incubated 72 hrs by CellTiter-Glo assay.	42911755
MRC5	CC₅₀	30.06 μM	Cytotoxicity against human MRC5 cells assessed as viability reduction incubated 48 hrs by CellTiter-Glo assay. Cytotoxicity against human MRC5 cells assessed as viability reduction incubated 48 hrs by CellTiter-Glo assay.	42911755
A549	CC₅₀	34.19 μM	Cytotoxicity against human A549 cells assessed as viability reduction incubated 72 hrs by CellTiter-Glo assay. Cytotoxicity against human A549 cells assessed as viability reduction incubated 72 hrs by CellTiter-Glo assay.	42911755
MDCK	CC₅₀	56.62 μM	Cytotoxicity against MDCK cells assessed as viability reduction incubated 48 hrs by CellTiter-Glo assay. Cytotoxicity against MDCK cells assessed as viability reduction incubated 48 hrs by CellTiter-Glo assay.	42911755
Vero	CC₅₀	5.22 μM	Cytotoxicity against VERO TMPRSS cells assessed as viability reduction incubated 24 hrs by WST-8 assay. Cytotoxicity against VERO TMPRSS cells assessed as viability reduction incubated 24 hrs by WST-8 assay.	42911755

In Vitro

Endoplasmic Reticulum StressViral InfectionOthers

Endoplasmic Reticulum Stress

AV22 (compound 1o) (25 μM) inhibits PDIA3 disulfide reductase activity with an IC₅₀ of 7.0 μM^[1].
AV22 also inhibits PDIA1 with an IC₅₀ of 19.4 μM, showing weaker potency than its inhibition of PDIA3^[1].
AV22 binds to PDIA3 with a K_d of 1.69 μM^[1].

Viral Infection

AV22 inhibits hCoV-OC43 in HCT-8 cells, with a VRA EC₅₀ of 0.058 μM and an FFRA EC₅₀ of 1.15 μM^[1].
AV22 inhibits hCoV-229E, RSV-A, IAV H1N1, IAV H3N2, IBV, and SARS-CoV-2 UK/Milan/Kraken in VRA, with EC₅₀ values of 0.046, 0.060, 0.749, 0.319, 0.343, 0.10, 0.54, and 0.31 μM^[1], respectively.

Others

AV22 (24-72 h) has CC₅₀ values of 31.75 μM, 30.06 μM, 34.19 μM, 56.62 μM, and 5.22 μM in HCT-8, MRC5, A549, MDCK, and VERO TMPRSS cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 465.25
Formula C₂₂H₁₃Cl₂F₃N₂O₂
SMILES

ClC1=CC(Cl)=CC=C1C2=CC(N(O)C(C(NC3=CC=CC=C3)=O)=C4)=C4C(C(F)(F)F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bononi G, et al. Development of N-Hydroxyindole-2-Carboxamides as New Host-Targeted Broad-Spectrum Antiviral Agents Against Human Respiratory Viruses. J Med Chem. 2026 Oct 22;69(20):24068-24095.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote