BAP9THP
Based on 1 Customer Validation
BAP9THP is a synthetic cytokinin derivative and a growth regulator. BAP9THP promotes chlorophyll retention (and senescence delay) in plant tissues exceptionally strongly, and growth of tobacco callus almost as strongly as 6-Benzylaminopurine (BAP). BAP9THP induces adventitious shoot formation ignificantly more strongly than N6-isopentenyladenine or Kinetin.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.92%
- No. CAS: 2312-73-4
- Fòrmula: C17H19N5O
- Peso molecular:309.37
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BJ | IC50 |
>100 μM
Compound: 1, PBA
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Cytotoxicity against human BJ cells after 72 hrs by MTT assay
Cytotoxicity against human BJ cells after 72 hrs by MTT assay
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[PMID: 19232496] |
| K562 | IC50 |
>100 μM
Compound: 1, PBA
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Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay
Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay
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[PMID: 19232496] |
| MCF7 | IC50 |
>100 μM
Compound: 1, PBA
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Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay
Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay
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[PMID: 19232496] |
In Vitro
The cytokinin derivative BAP9THP is an important component of these protocols: culture of isolated apical meristem or growing shoot apices on media containing this compound resulted in signifcant shoot multiplication[1].
both BAP9THP and BAP9THF are found to delay senescence and induce several growth responses more strongly than BAP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2312-73-4
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Appearance Solid
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Peso molecular 309.37
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Fòrmula C17H19N5O
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Color White to off-white
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SMILES
N1(C=NC2=C(N=CN=C12)NCC3=CC=CC=C3)C4CCCCO4
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Synonyms
SD 8339; N-Benzyl-9-(tetrahydro-2h-pyran-2-yl)adenine
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (323.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2324 mL | 16.1619 mL | 32.3238 mL | 80.8094 mL |
| 5 mM | 0.6465 mL | 3.2324 mL | 6.4648 mL | 16.1619 mL | |
| 10 mM | 0.3232 mL | 1.6162 mL | 3.2324 mL | 8.0809 mL | |
| 15 mM | 0.2155 mL | 1.0775 mL | 2.1549 mL | 5.3873 mL | |
| 20 mM | 0.1616 mL | 0.8081 mL | 1.6162 mL | 4.0405 mL | |
| 25 mM | 0.1293 mL | 0.6465 mL | 1.2930 mL | 3.2324 mL | |
| 30 mM | 0.1077 mL | 0.5387 mL | 1.0775 mL | 2.6936 mL | |
| 40 mM | 0.0808 mL | 0.4040 mL | 0.8081 mL | 2.0202 mL | |
| 50 mM | 0.0646 mL | 0.3232 mL | 0.6465 mL | 1.6162 mL | |
| 60 mM | 0.0539 mL | 0.2694 mL | 0.5387 mL | 1.3468 mL | |
| 80 mM | 0.0404 mL | 0.2020 mL | 0.4040 mL | 1.0101 mL | |
| 100 mM | 0.0323 mL | 0.1616 mL | 0.3232 mL | 0.8081 mL |
BAP9THP Related Classifications
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