Bathocuproine 

Bathocuproine is a phenanthroline chelator that binds specifically to Cu⁺. Bathocuproine preferentially binds Cu⁺ over Cu²⁺ to form a chromogenic complex, which is used to detect the oxidation state of copper in biomolecules. Bathocuproine undergoes concentration-dependent self-association to form dimers, and it can engage in aromatic stacking interactions with aromatic residues of Aβ (K_d ≈ 1 mM). Bathocuproine is applicable to research related to Alzheimer's disease^[1][2].

Bathocuproine (0.32-3.16 mM) undergoes reversible monomer-dimer self-association in aqueous solution at 25 °C, with an association constant of 0.64 mM^-1.
Bathocuproine (0.32-3.16 mM) binds to the Aβ40 peptide only in its dimeric form in aqueous solution at 25 °C, with a dissociation constant of approximately 1 mM under conditions where the dimer accounts for ≥ 40% of the total^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

360.46

C₂₆H₂₀N₂

4733-39-5

Solid

Light yellow to brown

N=1C(=CC(C=2C=CC=CC2)=C3C=CC4=C(N=C(C=C4C=5C=CC=CC5)C)C13)C

Room temperature in continental US; may vary elsewhere.

Store at room temperature 3 years

• [1]. Chen D, et al. An improved Bathocuproine assay for accurate valence identification and quantification of copper bound by biomolecules. Anal Biochem. 2016;497:27-35.  [Content Brief]

  [2]. Yao S, et al. Characterizing bathocuproine self-association and subsequent binding to Alzheimer's disease amyloid beta-peptide by NMR. J Pept Sci. 2004;10(4):210-217.  [Content Brief]