BI-1910
Based on 1 Customer Validation
BI-1910 is an agonistic human IgG2 monoclonal antibody targeting TNFR2, which selectively binds to human TNFR2 without inhibiting TNF-α binding. As an endogenous agonist, BI-1910 activates TNFR2 and mediates the activation of the NF-κB signaling pathway. Its agonistic activity is independent of FcγR or cross-linking; it can directly costimulate T cells and NK cells, induce T cell proliferation, and activate tumor-specific CD8+ T cells. BI-1910 exhibits antitumor activity in vivo, and shows an additive antitumor effect when combined with anti-PD-1 (HY-P9902). It can be used in research related to advanced/metastatic non-small cell lung cancer and hepatocellular carcinoma. Recommended isotype control: Human IgG2 lambda, Isotype Control (HY-P991206).
For research use only. We do not sell to patients.
- Purity: 98.24%
- Molecular Weight:143.27 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG2 lambda
Human
TNFRSF1B
BI-1910 (15 min) acts as an agonist of NF-κB signaling in TNFR1-deficient NK-925 cells, with enhanced activity in the presence of TNF-α[1].
BI-1910 (10 μg/mL; 24 h) acts as an agonist to increase IFN-γ production from primary human NK cells[1].
BI-1910 (10 μg/mL; 72 h) acts as an intrinsic agonist to promote proliferation of activated primary human CD4+ T cells, with activity independent of cross-linking[1].
BI-1910 (10 μg/mL; 30 min pre-incubation, followed by 72 h co-culture) delivers TNFR2-mediated co-stimulatory activity to CD3/CD28-stimulated primary human T cells co-cultured with TAMs[1].
BI-1910 (6 h) activates NF-κB signaling in TNFR2-expressing Jurkat reporter cells, as measured by GFP expression[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:primary human CD4+ T cells
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Concentration:10 μg/mL
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Incubation Time:72 h
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Result:Acted as a strong agonist and promoted the proliferation of primary human CD4+ T cells stimulated with plate-bound anti-CD3 and soluble anti-CD28. The agonistic activity was independent of antibody cross-linking.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J B-hTNFR2 (human TNFR2 knock-in) (female)
Female B-hTNFR2 knock-in mice (C57BL/6J background), 6-8 weeks old, were subcutaneously inoculated with 0.5 × 106 or 1 × 106 MC38 colon cancer cells suspended in 100 μL PBS into the flank; when tumors reached 40-200 mm3, the mice were randomized into treatment groups based on tumor size and treated via intraperitoneal injection at a dose of 40 mg/kg in a volume of 200 μL.
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Dosage:40 mg/kg
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Administration:i.p.
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Result:Increased tumor-infiltrating CD8+ T cells on day 8 post-treatment.
Reduced CD8+ T cell exhaustion (measured by fewer TOX+ cells) on day 8 post-treatment.
Increased proliferation of tumor-specific CD8+ T cells (measured by antigen-specific dextramers) on day 8 post-treatment.
Induced CD8+ T cell proliferation in tumor-draining lymph nodes on day 8 post-treatment.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay
Chemical Information
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Appearance Liquid
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Molecular Weight 143.27 kDa
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Color Colorless to light yellow
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SMILES
[BI-1910]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BI-1910 Related Classifications
HY-P991402 Related Classifications
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