Biotin-aniline
Based on 1 Customer Validation
Biotin-aniline is a probe with substantially high reactivity towards RNA and DNA. Biotin-aniline is also a novel APEX2 substrate. Biotin-aniline can label proteins via miniSOG. Biotin-aniline emerges as more efficient probe for capturing subcellular transcriptome in living cells with high spatial specificity.
For research use only. We do not sell to patients.
- Purity: 99.63%
- CAS No.: 769933-15-5
- Formula: C18H26N4O2S
- Molecular Weight:362.49
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Biotin-aniline is more reactive than Btn-Ph when labeling purified APEX2 protein[1].
Biotin-aniline yields strong labeling signal in live HEK293T cells stably expressing cytoplasmic targeted miniSOG[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 769933-15-5
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Appearance Solid
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Molecular Weight 362.49
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Formula C18H26N4O2S
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Color White to off-white
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SMILES
NC(C=C1)=CC=C1CCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 250 mg/mL (689.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhou Y, et al. Expanding APEX2 Substrates for Proximity-Dependent Labeling of Nucleic Acids and Proteins in Living Cells. Angew Chem Int Ed Engl. 2019;58(34):11763-11767. [Content Brief]
[2]. Zheng F, et al. Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome. Nat Commun. 2023 May 23;14(1):2978. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7587 mL | 13.7935 mL | 27.5870 mL | 68.9674 mL |
| 5 mM | 0.5517 mL | 2.7587 mL | 5.5174 mL | 13.7935 mL | |
| 10 mM | 0.2759 mL | 1.3793 mL | 2.7587 mL | 6.8967 mL | |
| 15 mM | 0.1839 mL | 0.9196 mL | 1.8391 mL | 4.5978 mL | |
| 20 mM | 0.1379 mL | 0.6897 mL | 1.3793 mL | 3.4484 mL | |
| 25 mM | 0.1103 mL | 0.5517 mL | 1.1035 mL | 2.7587 mL | |
| 30 mM | 0.0920 mL | 0.4598 mL | 0.9196 mL | 2.2989 mL | |
| 40 mM | 0.0690 mL | 0.3448 mL | 0.6897 mL | 1.7242 mL | |
| 50 mM | 0.0552 mL | 0.2759 mL | 0.5517 mL | 1.3793 mL | |
| 60 mM | 0.0460 mL | 0.2299 mL | 0.4598 mL | 1.1495 mL | |
| 80 mM | 0.0345 mL | 0.1724 mL | 0.3448 mL | 0.8621 mL | |
| 100 mM | 0.0276 mL | 0.1379 mL | 0.2759 mL | 0.6897 mL |
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