1. Anti-infection
  2. Bacterial
  3. BSH-IN-1

BSH-IN-1 

Cat. No.: HY-135659 Purity: ≥98.0%
Handling Instructions

BSH-IN-1 is a potent and covalent inhibitor of gut bacterial recombinant bile salt hydrolases (BSHs) with IC50s of 108 nM and 427 nM for B. longum BSH (Gram positive) and B. theta BSH (Gram negative), respectively.

For research use only. We do not sell to patients.

BSH-IN-1 Chemical Structure

BSH-IN-1 Chemical Structure

CAS No. : 2553217-91-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 528 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 528 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 480 In-stock
Estimated Time of Arrival: December 31
10 mg USD 800 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2200 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of BSH-IN-1:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BSH-IN-1 is a potent and covalent inhibitor of gut bacterial recombinant bile salt hydrolases (BSHs) with IC50s of 108 nM and 427 nM for B. longum BSH (Gram positive) and B. theta BSH (Gram negative), respectively[1].

In Vitro

BSH-IN-1 (Compound 7) also inhibits BSH activity in growing cultures of Gram-negative (B. theta VPI-5482, Bacteroides fragilis ATCC 25285, and Bacteroides vulgatus ATCC 8482) and Gram-positive (Lactobacillus plantarum WCFS1, C. perfringens ATCC 13124, and Bifidobacterium adolescentis L2-32) bacteria. BSH-IN-1 (Compound 7) also is a potent BSH inhibitor in growing bacterial cultures with IC50s of 237 nM and 1070 nM for B. adolescentis (Gram positive) and B. theta (Gram negative), respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

BSH-IN-1 (Compound 7; 10 mg/kg; a single dose; gavage) inhibits BSH activity in C57BL/6 mice and can be gut-restricted[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice[1]
Dosage: 10 mg/kg
Administration: Single gavage
Result: Inhibited gut bacterial BSH activity and modulated the bile acid pool in vivo while not significantly affected the gut bacterial community.
Molecular Weight

408.59

Formula

C25H41FO3

CAS No.
SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (61.19 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4474 mL 12.2372 mL 24.4744 mL
5 mM 0.4895 mL 2.4474 mL 4.8949 mL
10 mM 0.2447 mL 1.2237 mL 2.4474 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.12 mM); Clear solution

*All of the co-solvents are available by MCE.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
BSH-IN-1
Cat. No.:
HY-135659
Quantity:
MCE Japan Authorized Agent: