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  4. BTD probe-1

BTD probe-1 is a benzothiazine-based chemoproteomic probe and selective protein S-sulfenic acid (Cys-SOH) labeling agent. BTD probe-1 labels protein S-sulfenic acids in vitro in cell and tissue samples, and in situ in intact cells, enabling detection or enrichment of modified proteins/peptides. BTD probe-1 exhibits no cytotoxicity in cells at concentrations ≤1 mM. BTD probe-1 enables global, site-specific mapping and quantification of cysteine S-sulfenylation in complex proteomes with lower input material.

For research use only. We do not sell to patients.

BTD probe-1

BTD probe-1 Chemical Structure

CAS No. : 2095485-22-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

BTD probe-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BTD probe-1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BTD probe-1 is a benzothiazine-based chemoproteomic probe and selective protein S-sulfenic acid (Cys-SOH) labeling agent. BTD probe-1 labels protein S-sulfenic acids in vitro in cell and tissue samples, and in situ in intact cells, enabling detection or enrichment of modified proteins/peptides. BTD probe-1 exhibits no cytotoxicity in cells at concentrations ≤1 mM. BTD probe-1 enables global, site-specific mapping and quantification of cysteine S-sulfenylation in complex proteomes with lower input material[1].

In Vitro

Under conditions such as oxidative stress, the thiol group (-SH) of cysteine residues in proteins may be oxidized to S-sulfinic acid group (-SOH). BTD probe-1 can selectively react with this specific oxidized form to label it.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BTD probe-1 Related Antibodies
Molecular Weight

263.31

Formula

C13H13NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1CS(N(CCCC#C)C2=CC=CC=C12)(=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (379.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7978 mL 18.9890 mL 37.9781 mL
5 mM 0.7596 mL 3.7978 mL 7.5956 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.06%

SDS (251 KB)

COA (251 KB) HNMR (245 KB) RP-HPLC (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7978 mL 18.9890 mL 37.9780 mL 94.9451 mL
5 mM 0.7596 mL 3.7978 mL 7.5956 mL 18.9890 mL
10 mM 0.3798 mL 1.8989 mL 3.7978 mL 9.4945 mL
15 mM 0.2532 mL 1.2659 mL 2.5319 mL 6.3297 mL
20 mM 0.1899 mL 0.9495 mL 1.8989 mL 4.7473 mL
25 mM 0.1519 mL 0.7596 mL 1.5191 mL 3.7978 mL
30 mM 0.1266 mL 0.6330 mL 1.2659 mL 3.1648 mL
40 mM 0.0949 mL 0.4747 mL 0.9495 mL 2.3736 mL
50 mM 0.0760 mL 0.3798 mL 0.7596 mL 1.8989 mL
60 mM 0.0633 mL 0.3165 mL 0.6330 mL 1.5824 mL
80 mM 0.0475 mL 0.2374 mL 0.4747 mL 1.1868 mL
100 mM 0.0380 mL 0.1899 mL 0.3798 mL 0.9495 mL
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BTD probe-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BTD probe-12095485-22-4BTD probe1BTD probe 1Biochemical Assay Reagentscomplex proteomescell samplesintact cellscysteine S-sulfenylationCys-SOHprotein S-sulfenic acidtissue samplesInhibitorinhibitorinhibit

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