5,6-DiHETE
Based on 1 Customer Validation
5,6-DiHETE is an anti-inflammatory lipid mediator derived from eicosapentaenoic acid (EPA). 5,6-DiHETE has the ability to suppress vascular hyperpermeability during inflammation and is used in the research of inflammatory diseases.
For research use only. We do not sell to patients.
- Purity: 97.00%
- CAS No.: 845673-97-4
- Formula: C20H32O4
- Molecular Weight:336.47
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Chemical Information
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CAS No. 845673-97-4
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Appearance Oil
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Molecular Weight 336.47
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Formula C20H32O4
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Color Light yellow to yellow
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SMILES
CC/C=C\C/C=C\C/C=C\C/C=C\CC(O)C(O)CCCC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (297.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Misato Kida, et al. A novel eicosapentaenoic acid-derived anti-inflammatory lipid mediator 5,6-DiHETE is abundant in blue back fish intestines. J Food Sci. 2020, 85, 7. [Content Brief]
[2]. Kida M, et al. A novel eicosapentaenoic acid-derived anti-inflammatory lipid mediator 5,6-DiHETE is abundant in blue back fish intestines. J Food Sci. 2020 Jul;85(7):1983-1987. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9720 mL | 14.8602 mL | 29.7203 mL | 74.3008 mL |
| 5 mM | 0.5944 mL | 2.9720 mL | 5.9441 mL | 14.8602 mL | |
| 10 mM | 0.2972 mL | 1.4860 mL | 2.9720 mL | 7.4301 mL | |
| 15 mM | 0.1981 mL | 0.9907 mL | 1.9814 mL | 4.9534 mL | |
| 20 mM | 0.1486 mL | 0.7430 mL | 1.4860 mL | 3.7150 mL | |
| 25 mM | 0.1189 mL | 0.5944 mL | 1.1888 mL | 2.9720 mL | |
| 30 mM | 0.0991 mL | 0.4953 mL | 0.9907 mL | 2.4767 mL | |
| 40 mM | 0.0743 mL | 0.3715 mL | 0.7430 mL | 1.8575 mL | |
| 50 mM | 0.0594 mL | 0.2972 mL | 0.5944 mL | 1.4860 mL | |
| 60 mM | 0.0495 mL | 0.2477 mL | 0.4953 mL | 1.2383 mL | |
| 80 mM | 0.0372 mL | 0.1858 mL | 0.3715 mL | 0.9288 mL | |
| 100 mM | 0.0297 mL | 0.1486 mL | 0.2972 mL | 0.7430 mL |
5,6-DiHETE Related Classifications
HY-130138C Related Classifications
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