RI-1
Based on 7 publication(s) in Google Scholar
RI-1 is a RAD51 inhibitor, with IC50s ranging from 5 to 30 μM. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319. RI-1 inactivates RAD51 by directly binding to a protein surface that serves as an interface between protein subunits in RAD51 filaments. RI-1 can disrupt homologous recombination in human cells.
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 415713-60-9
- Formula: C14H11Cl3N2O3
- Molecular Weight:361.61
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) RI-1
More- Adv Mater. 2024 Sep 9:e2410031. [Abstract]
- Mol Cell. 2025 Sep 10:S1097-2765(25)00702-6. [Abstract]
- Cancer Lett. 2021 Aug 1:512:1-14. [Abstract]
- Neoplasia. 2019 Apr 24;21(6):533-544. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
- Hum Mol Genet. 2019 Oct 15;28(20):3422-3430. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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IF
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Others
Biological Activity
IC50: 5-30 μM (RAD51)[1]
RI-1 (1-50 μM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells[1].
RI-1 (5-20 μM; 30 min) inhibits HsRAD51 in a concentration-dependent manner[1].
RI-1 (20 μM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts[1].
RI-1 (15-25 μM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c nude mice (6 weeks) bearing TNBC tumor[2]
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Dosage:50 mg/kg
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Administration:I.p. every 3 days for 30 days
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Result:Resulted in significant inhibition of tumor growth.
Did not cause body weight loss significantly.
Chemical Information
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CAS No. 415713-60-9
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Appearance Solid
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Molecular Weight 361.61
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Formula C14H11Cl3N2O3
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Color Yellow to orange
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SMILES
O=C(C(N1CCOCC1)=C2Cl)N(C3=CC=C(Cl)C(Cl)=C3)C2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (7)
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Journal Impact Factor
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Most Recent
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Adv Mater
A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. [Abstract]2024 Sep 9:e2410031. PMID: 39246208 -
Mol Cell
2025 Sep 10:S1097-2765(25)00702-6. PMID: 40934926 -
Cancer Lett
XRCC2 repairs mitochondrial DNA damage and fuels malignant behavior in hepatocellular carcinoma. [Abstract]2021 Aug 1:512:1-14. PMID: 33964350
RI-1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Aug 1:512:1-14. [Abstract]
The colony formation survival rate of the RPE1 cells was quantitatively analyzed, and the effect of RAD51 (RI-1) dose was examined.
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Neoplasia
DAXX, as a Tumor Suppressor, Impacts DNA Damage Repair and Sensitizes BRCA-Proficient TNBC Cells to PARP Inhibitors. [Abstract]2019 Apr 24;21(6):533-544. PMID: 31029033
RI-1 purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Apr 24;21(6):533-544. [Abstract]
MDA-MB-157 cells treated with different concentrations of ABT888 for 72 hours in combination with DMSO or a constant dose of 25-50 μM RI-1.
RI-1 purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Apr 24;21(6):533-544. [Abstract]
RI-1 (50 mg/kg). The expression of RAD51 in these four groups of mice was analyzed by immunofluorescence.
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471 -
Hum Mol Genet
The repair of endo/exogenous DNA double-strand breaks and its effects on meiotic chromosome segregation in oocytes. [Abstract]2019 Oct 15;28(20):3422-3430. PMID: 31384951
RI-1 purchased from MedChemExpress. Usage Cited in: Hum Mol Genet. 2019 Oct 15;28(20):3422-3430. [Abstract]
The PBE rate of oocytes in the control group and the RI-1 (60 μM) treatment group was measured 11 to 16 hours after in vitro maturation.
Solvent & Solubility
DMSO : 33.33 mg/mL (92.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Budke B, et, al. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res. 2012 Aug;40(15):7347-57. [Content Brief]
[2]. Shi Y, et, al. DAXX, as a Tumor Suppressor, Impacts DNA Damage Repair and Sensitizes BRCA-Proficient TNBC Cells to PARP Inhibitors. Neoplasia. 2019 Jun;21(6):533-544. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7654 mL | 13.8271 mL | 27.6541 mL | 69.1353 mL |
| 5 mM | 0.5531 mL | 2.7654 mL | 5.5308 mL | 13.8271 mL | |
| 10 mM | 0.2765 mL | 1.3827 mL | 2.7654 mL | 6.9135 mL | |
| 15 mM | 0.1844 mL | 0.9218 mL | 1.8436 mL | 4.6090 mL | |
| 20 mM | 0.1383 mL | 0.6914 mL | 1.3827 mL | 3.4568 mL | |
| 25 mM | 0.1106 mL | 0.5531 mL | 1.1062 mL | 2.7654 mL | |
| 30 mM | 0.0922 mL | 0.4609 mL | 0.9218 mL | 2.3045 mL | |
| 40 mM | 0.0691 mL | 0.3457 mL | 0.6914 mL | 1.7284 mL | |
| 50 mM | 0.0553 mL | 0.2765 mL | 0.5531 mL | 1.3827 mL | |
| 60 mM | 0.0461 mL | 0.2305 mL | 0.4609 mL | 1.1523 mL | |
| 80 mM | 0.0346 mL | 0.1728 mL | 0.3457 mL | 0.8642 mL |
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