CSK-IN-1
Based on 4 publication(s) in Google Scholar
CSK-IN-1 (compound 13) is a potent, orally active c-terminal Src kinase (CSK) with IC50 values below 3 nM and 4 nM in CSK HTRF and Caliper assay, respectively. CSK-IN-1 shows the ability to increase T cell proliferation induced by T cell receptor signaling.
For research use only. We do not sell to patients.
- Purity: 98.04%
- CAS No.: 2380027-49-4
- Formula: C34H32ClFN8O4
- Molecular Weight:671.12
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CSK-IN-1
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Biological Activity
IC50: <3 nM (c-terminal Src kinase CSK in HTRF assay), 4 nM (c-terminal Src kinase in Caliper assay)[1]
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Cell Line
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Type | Value | Description | References |
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| Jurkat | EC50 |
41 nM
Compound: 13
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Effect on ZAP70 phosphorylation in human Jurkat cells pre-incubated for 30 mins followed by anti-CD3/CD28 stimulation measured after 10 mins
Effect on ZAP70 phosphorylation in human Jurkat cells pre-incubated for 30 mins followed by anti-CD3/CD28 stimulation measured after 10 mins
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[PMID: 31620238] |
Chemical Information
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CAS No. 2380027-49-4
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Appearance Solid
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Molecular Weight 671.12
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Formula C34H32ClFN8O4
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Color Light yellow to yellow
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SMILES
O=C(C1=NN(C2CCN(C(CC(C)(C#N)C)=O)CC2)C3=C1C=C(F)C=C3)NC4=CC=C(NC(C5=C6C=CC(OC)=CN6N=C5)=O)C(Cl)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jan;649(8098):1032-1041. PMID: 41299171 -
Nat Immunol
Kupffer cell calibration of T cell responses via VSIG4-CD5 interaction promotes tumor evasion. [Abstract]2026 Jun;27(6):1253-1267. PMID: 42056254 -
Int J Mol Sci
2021 Aug 27;22(17):9289. PMID: 34502195 -
Cell Signal
Annexin A2 binds the 3'-UTR of H2AX mRNA and regulates histone-H2AX-derived hypoxia-inducible factor 1-alpha activation. [Abstract]2025 Aug:132:111781. PMID: 40164417
Solvent & Solubility
DMSO : 125 mg/mL (186.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4900 mL | 7.4502 mL | 14.9005 mL | 37.2512 mL |
| 5 mM | 0.2980 mL | 1.4900 mL | 2.9801 mL | 7.4502 mL | |
| 10 mM | 0.1490 mL | 0.7450 mL | 1.4900 mL | 3.7251 mL | |
| 15 mM | 0.0993 mL | 0.4967 mL | 0.9934 mL | 2.4834 mL | |
| 20 mM | 0.0745 mL | 0.3725 mL | 0.7450 mL | 1.8626 mL | |
| 25 mM | 0.0596 mL | 0.2980 mL | 0.5960 mL | 1.4900 mL | |
| 30 mM | 0.0497 mL | 0.2483 mL | 0.4967 mL | 1.2417 mL | |
| 40 mM | 0.0373 mL | 0.1863 mL | 0.3725 mL | 0.9313 mL | |
| 50 mM | 0.0298 mL | 0.1490 mL | 0.2980 mL | 0.7450 mL | |
| 60 mM | 0.0248 mL | 0.1242 mL | 0.2483 mL | 0.6209 mL | |
| 80 mM | 0.0186 mL | 0.0931 mL | 0.1863 mL | 0.4656 mL | |
| 100 mM | 0.0149 mL | 0.0745 mL | 0.1490 mL | 0.3725 mL |
CSK-IN-1 Related Classifications
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