Cat. No.: HY-N7028

Withanolide A

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Withanolide A is an orally active extract from the Indian herb Ashwagandha. Withanolide A can induce apoptosis. Withanolide A has anti-inflammatory and antitumor activity. Withanolide A can be used in the study of neurodegenerative diseases.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 32911-62-9
Formula: C₂₈H₃₈O₆
Molecular Weight:470.60

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Withanolide A Chemical Structure

CAS No.: 32911-62-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H929	IC₅₀	>10 μM Compound: 8	Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay Cytotoxicity against human NCI-H929 cells after 3 days by Alamar blue assay	[PMID: 24625088]

In Vitro

Withanolide A (1 μM, 4 days) can induce presynaptic and postsynaptic reconstruction of rat neurons and induce significant regeneration of axons and dendrites^[1].
Withanolide A (0.5-2 μM, 24-48 h) inhibits growth of CaOV3 and SKOV3 ovarian cancer cells by targeting Notch1 and Notch3^[2].
Withanolide A (0.5-50 μg/mL, 3 h) has the potential of vascular relaxation by promoting the production of NO in endothelial cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

CaOV3、SKOV3、OVCAR3、TOV112D and TOV21G
Concentration:

0, 0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10 μM
Incubation Time:

72 h
Result:

Decreased the cell viability and with an IC₅₀ concentrations at 520 nM, 627 nM, 452 nM, 262 nM, 243 nM, respectively.

Apoptosis Analysis^[2]

Cell Line:

CaOV3, SKOV3
Concentration:

0.5, 1, 2 μM
Incubation Time:

24, 48 h
Result:

Induced apoptosis in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:

CaOV3, SKOV3
Concentration:

0.5, 1, 2 μM
Incubation Time:

24, 48 h
Result:

Induced G2/M phase cell cycle arrest.

Western Blot Analysis^[2]

Cell Line:

CaOV3, SKOV3
Concentration:

0.5, 1, 2 μM
Incubation Time:

24, 48 h
Result:

Decreased cdc25C protein.
Cyclin B1 stayed unchanged at 24 h but decreased by 48 h in a dose-dependent manner in CaOV3 cells, transiently increased in SKOV3 cells.
Reduced the levels of phospho-Akt (ser 473) and Bcl-2.
Reduced Notch 3 protein levels in CaOV3 cells and Notch1 protein levels in SKOV3 cells.

In Vivo

Withanolide A (10 μmol/kg, dissolved in 0.5% acacia solution for 21 days orally) prevents neurodegeneration in rats by modulating hippocampal glutathione biosynthesis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male Sprague Dawley rats weighing 240–250 g^[4]
Dosage:

10 μmol/kg
Administration:

p.o. (dissolved in 0.5% gum arabic solution)
Result:

Decreased the level of reactive oxygen species generation and lipid peroxidation.
Increased the level of GSH and the activity of glutathione reductase, glutathione s transferase and superoxide dismutase.
Increased the ATP, NADPH and GCLC activity.
Decreased corticosterone level and glucocorticoid receptor.

Chemical Information

CAS No. 32911-62-9
Appearance Solid
Molecular Weight 470.60
Formula C₂₈H₃₈O₆
Color White to off-white
SMILES

O=C1O[C@@]([H])([C@@](C)([C@H]2CC[C@]3([C@@]4([C@H]5[C@@H]([C@@](O)(CC=CC6=O)[C@]6([C@]4(CC[C@@]32C)[H])C)O5)[H])[H])O)CC(C)=C1C
Structure Classification
- Steroids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (21.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1249 mL

10.6247 mL

21.2495 mL

5 mM

0.4250 mL

2.1249 mL

4.2499 mL

10 mM

0.2125 mL

1.0625 mL

2.1249 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (2.12 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kuboyama T, et al. Neuritic regeneration and synaptic reconstruction induced by withanolide A. Br J Pharmacol. 2005 Apr;144(7):961-71.. [Content Brief]

[2]. Zhang X, et al. Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A. Gynecol Oncol. 2012 Mar;124(3):606-12. [Content Brief]

[3]. Pathak P, et al. Standardized root extract of Withania somnifera and Withanolide A exert moderate vasorelaxant effect in the rat aortic rings by enhancing nitric oxide generation. J Ethnopharmacol. 2021 Oct 5;278:114296. [Content Brief]

[4]. Baitharu I, et al. Withanolide A prevents neurodegeneration by modulating hippocampal glutathione biosynthesis during hypoxia. PLoS One. 2014 Oct 13;9(10):e105311. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1249 mL	10.6247 mL	21.2495 mL	53.1237 mL
	5 mM	0.4250 mL	2.1249 mL	4.2499 mL	10.6247 mL
	10 mM	0.2125 mL	1.0625 mL	2.1249 mL	5.3124 mL
	15 mM	0.1417 mL	0.7083 mL	1.4166 mL	3.5416 mL
	20 mM	0.1062 mL	0.5312 mL	1.0625 mL	2.6562 mL

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Withanolide A

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Neuronal Signaling Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Differentiation Inducing Compound Library

Lipid Compound Library

Medicine Food Homology Compound Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Aging Natural Product Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Cysteine Targeted Covalent Library

Cell Death Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Anti-Rheumatic Arthritis Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Withanolide A Related Classifications

HY-N7028 Related Classifications