BAM 15
Based on 12 publication(s) in Google Scholar
BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler.
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- Pureté: 99.82%
- CAS No.: 210302-17-3
- Formule: C16H10F2N6O
- Masse moléculaire:340.29
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Publications Citing Use of MedChemExpress (MCE) BAM 15
More- J Nanobiotechnology. 2026 Jan 24;24(1):169. [Abstract]
- Cell Rep Med. 2025 Jan 21;6(1):101890. [Abstract]
- Pharmacol Res. 2025 Nov:221:107973. [Abstract]
- Int J Biol Macromol. 2022 Aug 1:214:697-707. [Abstract]
- Cancer Immunol Res. 2025 Apr 22. [Abstract]
- Biochem Pharmacol. 2022 Apr:198:114948. [Abstract]
- Commun Biol. 2026 Jan 8;9(1):191. [Abstract]
- Sci Rep. 2025 Apr 17;15(1):13347. [Abstract]
- bioRxiv. 2025 March 11.
- bioRxiv. 2024 September 23.
- bioRxiv. 2024 November 05.
- bioRxiv. 2024 September 09.
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Cell Proliferation/Viability Assay
Activité biologique
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Cell Line
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Type | Value | Description | References |
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| L6 | EC50 |
0.27 μM
Compound: BAM15
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Induction of mitochondrial uncoupling in rat L6 cells assessed as stimulation of cellular oxygen consumption
Induction of mitochondrial uncoupling in rat L6 cells assessed as stimulation of cellular oxygen consumption
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[PMID: 26119501] |
| L6 | EC50 |
0.31 μM
Compound: 8; BAM15
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Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
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[PMID: 32392051] |
| L6 | EC50 |
0.31 μM
Compound: BAM15
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Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
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[PMID: 35907607] |
| L6 | EC50 |
0.49 μM
Compound: 5; BAM15
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Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
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[PMID: 32017849] |
| MCF7 | EC50 |
0.31 μM
Compound: BAM15
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Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
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[PMID: 33569941] |
| MDA-MB-231 | IC50 |
1.4 μM
Compound: 179
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Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay
Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay
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[PMID: 37300915] |
BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 210302-17-3
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Appearance Solid
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Masse moléculaire 340.29
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Formule C16H10F2N6O
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Color Light yellow to yellow
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SMILES
FC(C=CC=C1)=C1NC2=NC3=NON=C3N=C2NC4=C(C=CC=C4)F
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (12)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Renal-tubular-mitochondrial sequentially targeted nanoagent breaks the vicious cycle of oxidative stress and mtDNA-driven inflammation in acute kidney injury therapy. [Abstract]2026 Jan 24;24(1):169. PMID: 41580683 -
Cell Rep Med
2025 Jan 21;6(1):101890. PMID: 39793570
BAM 15 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Jan 21;6(1):101890. [Abstract]
BAM 15 (16 h) effectively decreased SKP2 expression in prostate cancer cells.
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Pharmacol Res
Imaging of mitochondrial matrix pH dynamics reveals a functional interaction between the ADP/ATP carrier and ATP synthase to regulate H+ distribution. [Abstract]2025 Nov:221:107973. PMID: 41015326
BAM 15 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Nov:221:107973. [Abstract]
H+ current densities at −160 mV in heart mitoplasts with different BAM 15 (0.25-5 µM) concentrations.
BAM 15 purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2025 Nov:221:107973. [Abstract]
Representative time course of the changes in fluorescence evoked by BAM 15 (500 nM) in WT C2C12 cells expressing SE-pHluorin targeted to the mitochondrial matrix. Mitochondrial matrix acidification due to BAM15 induced alkalinization.
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Int J Biol Macromol
Self-assembly drug-albumin nanocomposites for nonalcoholic fatty liver disease treatment. [Abstract]2022 Aug 1:214:697-707. PMID: 35777511 -
Cancer Immunol Res
2025 Apr 22. PMID: 40261130
BAM 15 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Res. 2025 Apr 22. [Abstract]
OCR measurement in control and MLN4924-treated activated human CD8+ T cells use BAM 15 (2.5 μmol/L) to induce maximal respiration.
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Biochem Pharmacol
The new mitochondrial uncoupler BAM15 induces ROS production for treatment of acute myeloid leukemia. [Abstract]2022 Apr:198:114948. PMID: 35192847
BAM 15 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2022 Apr:198:114948. [Abstract]
Four leukemia cell lines, U937, Kasumi1, THP1, and C1498, were incubated with BAM 15 (0-10 μM; 72 h) on a 96-well plate and the activity changes of the cell lines were measured by the CCK-8 method.
BAM 15 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2022 Apr:198:114948. [Abstract]
The infiltration potential of leukemia cells in the drug-added and vehicle groups was evaluated by H&E staining. Tumor cell invasion in the BAM 15 (5 mg/kg; i.p.; Once every two days; three weeks) group was significantly reduced.
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Commun Biol
Multimodal epigenetic and enhancer network remodeling shape the transcriptional landscape of human beige adipocytes. [Abstract]2026 Jan 8;9(1):191. PMID: 41501500 -
Sci Rep
Combining RNA-seq, molecular docking and experimental verification to explore the mechanism of BAM15 as a potential drug for atherosclerosis. [Abstract]2025 Apr 17;15(1):13347. PMID: 40247008 -
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Solvant et solubilité
DMSO : 50 mg/mL (146.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10 mM pyruvate, 2 mM malate, and 5 μM BAM 15 or FCCP. Rotenone (2 μM), succinate (10 mM), antimycin A (4 μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100 μM) plus ascorbate (10 mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5 mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (279 KB)
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SDS (760 KB)
- English - EN (760 KB)
- Français - FR (760 KB)
- Deutsch - DE (760 KB)
- Norwegian - NO (760 KB)
- Español - ES (760 KB)
- Swedish - SV (760 KB)
- Italian - IT (760 KB)
- Korean - KR (760 KB)
- Portuguese - PT (760 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9387 mL | 14.6933 mL | 29.3867 mL | 73.4667 mL |
| 5 mM | 0.5877 mL | 2.9387 mL | 5.8773 mL | 14.6933 mL | |
| 10 mM | 0.2939 mL | 1.4693 mL | 2.9387 mL | 7.3467 mL | |
| 15 mM | 0.1959 mL | 0.9796 mL | 1.9591 mL | 4.8978 mL | |
| 20 mM | 0.1469 mL | 0.7347 mL | 1.4693 mL | 3.6733 mL | |
| 25 mM | 0.1175 mL | 0.5877 mL | 1.1755 mL | 2.9387 mL | |
| 30 mM | 0.0980 mL | 0.4898 mL | 0.9796 mL | 2.4489 mL | |
| 40 mM | 0.0735 mL | 0.3673 mL | 0.7347 mL | 1.8367 mL | |
| 50 mM | 0.0588 mL | 0.2939 mL | 0.5877 mL | 1.4693 mL | |
| 60 mM | 0.0490 mL | 0.2449 mL | 0.4898 mL | 1.2244 mL | |
| 80 mM | 0.0367 mL | 0.1837 mL | 0.3673 mL | 0.9183 mL | |
| 100 mM | 0.0294 mL | 0.1469 mL | 0.2939 mL | 0.7347 mL |
BAM 15 Related Classifications
HY-110284 Related Classifications
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