Fura-2 AM
Based on 9 publication(s) in Google Scholar
Fura-2 AM is a membrane permeable, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator. Fura-2 AM crosses cell membranes and is converted to Fura-2 (HY-D0110A) via cellular esterases. Fura-2 AM can be used to detect calcium in cells.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.53%
- CAS No.: 108964-32-5
- Formule: C44H47N3O24
- Masse moléculaire:1001.85
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Stockage:
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-15924
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HY-D0815
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HY-D0814
DAPI dihydrochloride
Publications Citing Use of MedChemExpress (MCE) Fura-2 AM
More- Neuron. 2026 Mar 30:S0896-6273(26)00138-8. [Abstract]
- EMBO J. 2026 Apr;45(7):2182-2209. [Abstract]
- Sci Rep. 2025 May 8;15(1):16053. [Abstract]
- Purinergic Signal. 2024 Nov 16. [Abstract]
- Purinergic Signal. 2024 Jun;20(3):273-284. [Abstract]
- Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Abstract]
- bioRxiv. 2026 Jan 13.
- Elife. 2021 Mar 24:10:e64830. [Abstract]
- bioRxiv. 2021 Jan 13.
Customer Validation & Images
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Others
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Others
Activité biologique
Description
In Vitro
Guideline (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific requirements).
Preparation of Stock Solution
Add anhydrous DMSO to Fura-2 AM, pipette gently or vortex until completely dissolved to prepare a 1 mM stock solution.
Cell Loading
2.1 Preparation of Working Solution (prepare freshly before use):
Preheat serum-free or low-serum medium to 37℃, add the above 1 mM stock solution, and mix by gentle inversion to prepare a 1 μM loading working solution.
(Optional) To facilitate uniform intracellular dye entry, 0.02% Pluronic F-127 or 0.1% BSA can be added.
2.2 Loading Incubation:
Transfer the coverslips with neuronal cells to a 35 mm culture dish containing the working solution.
Incubate at 37℃ for 30 minutes in the dark.
2.3 Post-incubation (De-esterification):
Preheat 2 mL of fresh dye-free culture medium.
Take out the coverslips from the loading solution and transfer them into the fresh medium.
Continue incubation at 37℃ for 20–30 minutes in the dark. This step is critical to remove extracellular residual dye and ensure sufficient intracellular hydrolysis of the dye into free Fura-2 acid.
3. Pre-imaging Preparation
3.1 Washing: Gently rinse the coverslips 1–2 times with prewarmed HBSS or imaging buffer (containing Ca2?/Mg2?) to eliminate background dye.
3.2 Mounting: Mount the coverslip onto the imaging chamber and add 1–2 mL of imaging buffer.
3.3 Stabilization: Place the chamber on a thermostatted microscope stage (37℃), equilibrate for 5–10 minutes before baseline recording.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
505
Excitation (Ex)
336
Chemical Information
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CAS No. 108964-32-5
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Appearance Liquid (Density: 1.400±0.06 g/cm3)
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Masse moléculaire 1001.85
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Formule C44H47N3O24
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Color Light yellow to green yellow
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SMILES
O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O
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Synonyms
Fura-2 Acetoxymethyl ester
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (9)
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Journal Impact Factor
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Most Recent
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Neuron
Piezo1-mediated mechanotransduction in choroid plexus epithelial cells governs ciliogenesis and cerebrospinal fluid homeostasis. [Abstract]2026 Mar 30:S0896-6273(26)00138-8. PMID: 41916280 -
EMBO J
2026 Apr;45(7):2182-2209. PMID: 41714729 -
Sci Rep
Estrogen regulates duodenal calcium absorption and improves postmenopausal osteoporosis by the effect of ERβ on PMCA1b. [Abstract]2025 May 8;15(1):16053. PMID: 40341576 -
Purinergic Signal
Antagonism of the ATP-gated P2X7 receptor inhibits the proliferation of hepatocellular carcinoma cells. [Abstract]2024 Nov 16. PMID: 39549156 -
Purinergic Signal
Potential role of the P2X7 receptor in the proliferation of human diffused large B-cell lymphoma. [Abstract]2024 Jun;20(3):273-284. PMID: 37222921
Fura-2 AM purchased from MedChemExpress. Usage Cited in: Purinergic Signal. 2024 Jun;20(3):273-284. [Abstract]
The Ca2+ influx function of P2X7 in OCI-ly3 and SU-DHL-4 was validated by measurement of intracellular free Ca2+ concentration upon activation. Cells were pre-treated with 20 µΜ A740003 or control lock’s solution for 5 min respectively before administration with Bz-ATP. Triangle symbol indicates the addition of 50 µΜ Bz-ATP. The Fura-2 fluorescence at emission wavelength of 510 nm excited separately by wavelength of 340 or 380 nm were recorded, and the excitation ratio (340/380) was quantified at the indicated time point.
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Biochem Biophys Res Commun
2020 Dec 17;533(4):1276-1282. PMID: 33097186 -
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Elife
Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers. [Abstract]2021 Mar 24:10:e64830. PMID: 33760729
Fura-2 AM purchased from MedChemExpress. Usage Cited in: Elife. 2021 Mar 24:10:e64830. [Abstract]
Intracellular Ca2 levels of HEK293 cells expressing TRPR rose sharply in response to TRP2 and TRP3, based on fluorescence measures of the Ca2+ indicator Fura-2 AM. Hepes-buffered medium (HBM) was used as a control.
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Solvant et solubilité
In Vitro:
DMSO : 10 mg/mL (9.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9982 mL | 4.9908 mL | 9.9815 mL | 24.9538 mL |
| 5 mM | 0.1996 mL | 0.9982 mL | 1.9963 mL | 4.9908 mL |
Fura-2 AM Related Classifications
HY-101897 Related Classifications
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