Cat. No.: HY-N0731

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Genkwanin

(Synonyms: Puddumetin)

9 Cited Publications

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Based on 9 publication(s) in Google Scholar

Genkwanin is a major non-glycosylated flavonoid with anti-flammatory activities.

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Pureté: 99.52%
CAS No.: 437-64-9
Formule: C₁₆H₁₂O₅
Masse moléculaire:284.26

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Genkwanin Chemical Structure

CAS No.: 437-64-9

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (9)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Genkwanin is a major non-glycosylated flavonoid with anti-flammatory activities.

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	>20 μg/mL Compound: 4',5-dihydroxy-7-methoxyflavone	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 15217279]
A549	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 31784199]
Col2	ED₅₀	>20 μg/mL Compound: genkwanin	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 17279798]
HCT-15	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 31784199]
HeLa	IC₅₀	8.1 μM Compound: Genkwanin	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784199]
HUVEC	ED₅₀	>20 μg/mL Compound: genkwanin	Cytotoxicity against human HUVEC cells Cytotoxicity against human HUVEC cells	[PMID: 17279798]
LNCaP	ED₅₀	>20 μg/mL Compound: genkwanin	Cytotoxicity against human LNCaP cells Cytotoxicity against human LNCaP cells	[PMID: 17279798]
Lu1	ED₅₀	>20 μg/mL Compound: genkwanin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 17279798]
MCF-10A	IC₅₀	75 μM Compound: Genkwanin	Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MCF7	IC₅₀	10.3 μM Compound: Genkwanin	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784199]
MDA-MB-468	IC₅₀	1.6 μM Compound: Genkwanin	Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MOLM-13	IC₅₀	>10 μM Compound: 16	Cytotoxicity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MOLM-13 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026809]
MV4-11	IC₅₀	>10 μM Compound: 16	Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026809]
P388	IC₅₀	10 μg/mL Compound: 6, genkwanin	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 8759170]
RAW264.7	IC₅₀	5.64 μM Compound: 14	Anti-inflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NO production incubated for 24 hrs by Griess reagent based assay Anti-inflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NO production incubated for 24 hrs by Griess reagent based assay	[PMID: 37683361]
SK-MEL-2	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 31784199]
SK-OV-3	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 31784199]
TERT-RPE1	ED₅₀	3.9 μg/mL Compound: genkwanin	Cytotoxicity against human TERT-RPE1 cells Cytotoxicity against human TERT-RPE1 cells	[PMID: 17279798]
XF498	IC₅₀	0.4 μg/mL Compound: Genkwanin	Cytotoxicity against human XF498 cells by SRB assay Cytotoxicity against human XF498 cells by SRB assay	[PMID: 31784199]

In Vitro

Cell viability analysis shows that Genkwanin does not affect the cell viability up to a concentration of 50 μM. Genkwanin inhibits the LPS-induced production of NO in a concentration-dependent manner. iNOS only expresses in the present of external stimulus. Genkwanin could not significantly affect the activity of iNOS. The effect of Genkwanin on the production of proinflammatory cytokines is examined. Genkwanin suppresses the productions of TNF-a, IL-1b and IL-6 in LPS stimulated RAW264.7 macrophages in a concentration-dependent manner. Genkwanin significantly suppresses the AP-1 signaling pathway but has little effect on the NF-kB signaling pathway. It is indicated that Genkwanin suppresses the phosphorylation of p38 and JNK in a concentration-dependent manner, but little affects ERK1/2 phosphorylation. Originally identified as an immediate early gene, MKP-1 is then found to be a dual specificity phosphatase acting as a negative regulator of ERK1/2, JNK and p38 MAPK activities, with predominant effects on the latter two. Pretreatment with Genkwanin markedly up-regulates the expression of MKP-1 without affecting MKP-1 mRNA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 437-64-9
Appearance Solid
Masse moléculaire 284.26
Formule C₁₆H₁₂O₅
Color Light yellow to yellow
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=CC(OC)=CC(O)=C13
Synonyms

Puddumetin
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (9)

All Histological Imaging/StainingWBFlow CytometryIFCell Proliferation/Viability Assay

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Journal Impact Factor

Phytomedicine

Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice. [Abstract]

2025 Sep 24:148:157306. PMID: 41046685

Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]

HE staining analysis of pathological damage in the heart, liver, lung, and kidney tissues of mice treated with H-GKA (20 mg/kg, i.p.).

Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]

DYRK1A expression in lung tissues of asthmatic mice and mice after GKA (5, 10, 20 mg/kg, i.p.) treatment was examined using immunoblotting.

Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]

Detection of ROS in lung tissues of mice by DCFH-DA with GKA (5, 10, 20 mg/kg, i.p.) treatment.

Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]

DYRK1A expression in lung tissues of asthmatic mice and mice after GKA (5, 10, 20 mg/kg, i.p.) treatment was examined using g dual-labeling immunofluorescence.

Genkwanin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 24:148:157306. [Abstract]

The cell viability of BEAS-2B cells was examined using CCK-8 assays after GKA (30 μM) treatment.
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Eur J Pharmacol

Genkwanin blocks the interaction between phosphorylated JNK and NFATc1 to promote osteogenic differentiation and collagen Ⅰ α1 production. [Abstract]

2025 Jul 15:999:177687. PMID: 40311832
Int Immunopharmacol

Genkwanin alleviates intervertebral disc degeneration via regulating ITGA2/PI3K/AKT pathway and inhibiting apoptosis and senescence. [Abstract]

2024 May 30:133:112101. PMID: 38640717
Naunyn Schmiedebergs Arch Pharmacol

Genkwanin alleviates paraquat-induced acute lung injury by activating SIRT1-mediated Nrf2/HO-1 signaling pathway. [Abstract]

2025 Oct 17. PMID: 41105248
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biol Pharm Bull

Regioselective Glucuronidation of Flavones at C5, C7, and C4' Positions in Human Liver and Intestinal Microsomes: Comparison among Apigenin, Acacetin, and Genkwanin. [Abstract]

2022;45(8):1116-1123. PMID: 35908893
Aging (Albany NY)

A network pharmacology approach to uncover the key ingredients in Ginkgo Folium and their anti-Alzheimer's disease mechanisms. [Abstract]

2021 Jul 27;13(14):18993-19012. PMID: 34315132

Solvant et solubilité

In Vitro:

DMSO : 31.25 mg/mL (109.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5179 mL

17.5895 mL

35.1791 mL

5 mM

0.7036 mL

3.5179 mL

7.0358 mL

10 mM

0.3518 mL

1.7590 mL

3.5179 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.5 mg/mL (1.76 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 0.5 mg/mL (1.76 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.76 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (273 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Gao Y et al. Genkwanin inhibits proinflammatory mediators mainly through the regulation of miR-101/MKP-1/MAPK pathway in LPS-activated macrophages. PLoS One. 2014 May 6;9(5):e96741. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5179 mL	17.5895 mL	35.1791 mL	87.9477 mL
	5 mM	0.7036 mL	3.5179 mL	7.0358 mL	17.5895 mL
	10 mM	0.3518 mL	1.7590 mL	3.5179 mL	8.7948 mL
	15 mM	0.2345 mL	1.1726 mL	2.3453 mL	5.8632 mL
	20 mM	0.1759 mL	0.8795 mL	1.7590 mL	4.3974 mL
	25 mM	0.1407 mL	0.7036 mL	1.4072 mL	3.5179 mL
	30 mM	0.1173 mL	0.5863 mL	1.1726 mL	2.9316 mL
	40 mM	0.0879 mL	0.4397 mL	0.8795 mL	2.1987 mL
	50 mM	0.0704 mL	0.3518 mL	0.7036 mL	1.7590 mL
	60 mM	0.0586 mL	0.2932 mL	0.5863 mL	1.4658 mL
	80 mM	0.0440 mL	0.2199 mL	0.4397 mL	1.0993 mL
	100 mM	0.0352 mL	0.1759 mL	0.3518 mL	0.8795 mL

Keywords

Genkwanin

9 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Genkwanin

Activité biologique

Chemical Information

Publications (9)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Genkwanin Related Classifications

HY-N0731 Related Classifications